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Bacteriology > Lab 7 - Salmonella > Flashcards

Lab 7 - Salmonella Flashcards

1
Q

Salmonella serotypes can be found in diseased animals only.

A

False

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2
Q

S. enteritidis is a fastidious bacterium species

A

False, (non-fastidious)

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3
Q

S. Enteritidis is a bacterial species name.

A

False, (salmonella enterica)

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4
Q

Yersinia pseudotuberculosis can infect swine.

A

True

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5
Q

O9 serogroup of Yersinia pseudotuberculosis can cause cross-reactions with Brucella species in
serological tests.

A

False (O9 serogroups of Y. Enterocolitica)

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6
Q

Human typhus is caused by S. Typhimurium

A

False, (Rickettsia bacteria)

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7
Q

S. Enteritidis is a serotype name.

A

True

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8
Q

Salmonella are fermentative in OF (oxidative fermentation)-test.

A

True

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9
Q

Salmonella are widespread in our environment.

A

True

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10
Q

Foot rot of sheep is caused by Dichelobacter nodosus and F. necrophorum funduliforme.

A

False, (D. Nodus and F. Necrophorum subsp. Necroforum)

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11
Q

Fusobacteria are obligate aerobic bacteria.

A

False, (obligate anaerobic)

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12
Q

Shigella species are flagellated.

A

False, (no flagella)

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13
Q

Yersinia pseudotuberculosis cannot produce flagella at room temperature.

A

False, (flagella at room temperature, 22°C)

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14
Q

Salmonella enterica is a lactose-positive species.

A

False, (lactose negative)

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15
Q

S. Choleraesuis is the causative agent of swine typhus.

A

False, (S. typhisuis, causes necrosis in spleen and lungs)

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16
Q

Rodentiosis is caused by Yersinia enterocolitica.

A

False, (Yersinia pseudotuberculosis)

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17
Q

Serattia marcescens can produce pigments in low temperature

A

True

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18
Q

Fusobacteria need CO2 during culturing

A

True

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19
Q

S. Typhisuis, S. Gallinarum, (S. Pullorum) serotypes can only affect animals.

A

True

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20
Q

S. Typhi, S. Paratyphi A, B, C, S. Sendai serotypes can only affect humans.

A

True

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21
Q

Proteus is phenylalanine deaminase positive.

A

True

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22
Q

There is only one salmonella species

A

False. Two: Salmonella Enterica (further divided into six subspecies) and Salmonella Bongori)

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23
Q

Y. pseudotuberculosis can affect turkeys.

A

True

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24
Q

Optimal temperature for Yersinia is 42°C.

A

False

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25
Optimal temperature for Yersinia pseudotuberculosis is 30°C.
True
26
The necrobacillosis in the rabbit is caused by Fusobacterium necrophorum subsp. funduliforme.
False, (F. necrophorum subsp. necrophorum)
27
Necrobacillosis is caused by Salmonella Typhi
False, (fusobacterium necrophorum ssp. Necrophorum)
28
S. Pullorum is flagellated.
False, (no flagella)
29
Swine typhus is caused by Salmonella Typhi.
False, salmonella Typhi is only human. (Salmonella typhisuis – animals)
30
S. Gallinarum is flagellated.
False, (no flagella)
31
Salmonella are fastidious bacteria, therefore selective enrichment is needed for the isolation
False (not fastidious)
32
S. Cholerasuis can infect humans
True
33
The genus Salmonella and Citrobacter can be differentiated by catalase test
False?
34
Shigella cause bacillary dysentery to swine
False (only humans)
35
Proteus bacteria are able to propogate post mortem in animals bodies and other rotting material.
True?
36
S. tyhphi are causes typhus to humans, and paratyphus to animals
False (only humans)
37
O9 serogroup of Yersinia enterocolitica can cross react with Brucella species
True
38
The causative agent of Rodentiosis can infect birds, tyrkeys, pheasant and quail.
False (Y.pseudotuberculosis)
39
Edwarsiella tarda of Fusobacterium necrophorum is good.
False?
40
The resistance of Fusobacterium necrophorum is good.
False (Fusobacterium has weak resistance)
41
Dichelobacter nodosus is obligate aerobic bacteria
False (obligate anaerobic)
42
SALMONELLA species
- S. enterica | - S. bongori
43
Subspecies within S. enterica
* 6 subspecies * S. enterica subsp. enterica * S. enterica subsp. arizonae
44
SALMONELLA 1. Habitat 2. Morphology 3. Staining
``` 1. Habitat: • gut, • sewage, water, • feed!, • food 2. Morphology: • 1-5 µm rod, flagella • except: S. Gallinarum / S. Pullorum 3. Staining: • Gram negative ```
45
SALMONELLA | 4. Culture
* not fastidious * selective and differential media * low selective capacity (MacConkey, Drigalski) * medium selective capacity (brillant green-lactose- phenol red; XLD: xylose-lysine decarboxilase, Rambach agar) * high selective capacity * selective enrichment * Na-selenite, cystine * Na-tetrathionate, bile salts * Rappaport-Vassiliadis (ph 5.2; higher osmotic pressure)
46
SALMONELLA | 5. Biochemistry
``` • lactose -, • H2S + • differentiation of antigenically uniform types: sugar fermentation - Arabinose / Dulcitol / Mannitol: •S. Typhisuis: + / + / – •S. Choleraesuis: - / - / + ```
47
SALMONELLA | 6. Antigens
* Kauffmann-White O-Vi-H * O antigens: 51 O-groups, composite antigens * K (Vi): S. Typhi, S. Dublin, etc. * H antigens: * specific (phase 1) * non specific (phase 2) * monophasic types: only specific H antigens * diphasic types: not only specific H antigens
48
SALMONELLA | 7. Resistance
medium
49
SALMONELLA 8. Pathogenicity: a) General pathogenecity b) Which subsp. only affect humans? c) b) Which subsp. only affect animals? d) Which subsp. affect both humans and animals?
a) pathogenic - facultative pathogenic b) only human: S. Typhi, S. Paratyphi A, B, C, S. Sendai c) only animals: S. Typhisuis, S. Gallinarum, (S. Pullorum) d) human-animal: •S. Enteritidis •S. Typhimurium •S. Thompson •S. Choleraesuis •abortion: S. Abortusequi, S. Abortusovis, S. Abortusbovis
50
CITROBACTER 1. Habitat 2. Biochemistry 3. PAthogenecity
1. Habitat: mammals, birds, gut, water, environment 2. Biochemistry: • lactose – (late +) • KCN+, • lysine decarboxylase - 3. Pathogenicity: saprophytic, (secondary pathogenic)
51
SHIGELLA 1. Habitat 2. Morphology 3. Culture 4. Biochemistry 5. Pathogenicity
1. Habitat: intestine (primates), sewage 2. Morphology: no flagella 3. Culture: DC 4. Biochemistry: resembles E. coli, but lactose negative or late fermentation 5. Pathogenicity: S. dysenteriae, S. flexneri, S. sonnei, S. boydii: human bacillary, dysentery
52
PROTEUS, PROVIDENCIA 1. Habitat 2. Staining 3. Culture
1. Habitat: intestine, urine, water, rotting materials 2. Staining: Gram-negative 3. Culture: swarming!
53
PROTEUS, PROVIDENCIA | 4. Biochemistry
* lactose – * phenylalanine-desaminase +, * indole +/-, * urease +
54
PROTEUS, PROVIDENCIA | 5. Pathogenicity
* saprophytic, (urinary tract infection) * P. vulgaris, * P. mirabilis, * P. morganii, * Prov. stuartii
55
YERSINIA | Species
* Y. pestis * Y. pseudotuberculosis * Y. enterocolitica * Y. ruckeri
56
Y. pseudotuberculosis 1. Habitat 2. Morphology 3. Staining
1. Habitat: intestine, water 2. Morphology: 1-2 µm coccoid rods, flagella 22oC 3. Staining: Gram -, bipolar staining
57
Y. pseudotuberculosis 4. Culture 5. Biochemistry
``` • not fastidious • optimal temperature 30 oC, • replication: 4-42 oC, • small colonies • selective culture: • Enterobacteriaceae low selective capacity media 5. Biochemistry: lactose - ```
58
``` Which of these species are Indole postive? Salmonella Citrobacter Shigella Proteus Yersinia ```
Proteus and Yersinia are +/-
59
``` Which of these species are Urease postive? Salmonella Citrobacter Shigella Proteus Yersinia ```
Proteus and Yersinia
60
``` Which of these species are Lactose postive? Salmonella Citrobacter Shigella Proteus Yersinia ```
All are lactose negative
61
``` Which of these species are Mannitol postive? Salmonella Citrobacter Shigella Proteus Yersinia ```
``` Salmonella: + Citrobacter: + Shigella: negative Proteus: -/+ Yersinia: + ```
62
``` Which of these species are Phenylalanine-desaminase postive? Salmonella Citrobacter Shigella Proteus Yersinia ```
Proteus
63
``` Which of these species are Metilvörös postive? Salmonella Citrobacter Shigella Proteus Yersinia ```
All
64
``` Which of these species are Voges Proskauer postive? Salmonella Citrobacter Shigella Proteus Yersinia ```
All are neg.
65
``` Which of these species are H2S postive? Salmonella Citrobacter Shigella Proteus Yersinia ```
Salmonella, Citrobacter and Proteus (+/-)
66
Y. pseudotuberculosis | 6. Antigens
* O antigen: 6 serogroups, * 5 H antigens * composite antigens
67
Y. pseudotuberculosis | 7. Pathogenicity
* rodents (rodentiosis), * turkey, pheasant, quail (septicaemia, abscesses), * cattle, swine, sheep (abortion), human
68
Y. enterocolitica 1. Habitat 2. Morphology 3. Staining
1. Habitat: intestine, water 2. Morphology: middle sized rods 3. Staining: Gram negative
69
Y. enterocolitica | 4. Culture
* optimal temperature: 22 oC * selective culture: * Na-oxalate + bile salts
70
Y. enterocolitica | 4. Antigens
* O antigens: * 34 O-groups, * O9: cross-reaction with Brucella-species * H antigens * 20 H antigens
71
Y. enterocolitica | 5. Pathogenicity
* human: * gastro-intestinal infection, abscesses * swine, rabbit: * intestine, mesentherial lymph nodes, * resembles tuberculosis
72
Y. ruckeri 1. Biochemistry 2. Pathogenicity
1. Biochemistry: inactive | 2. Pathogenicity: trout septicaemia, kidney (red mouth disease)
73
SERRATIA 1. Habitat 2. Culture 3. Pathogenecity
1. Habitat: soil, water 2. Culture: lower optimal temperature, pigment 3. Pathogenicity: saprophytes • S. marcescens: food contamination
74
EDWARDSIELLA 1. Habitat 2. Culture 3. Pathogenecity
1. Habitat: intestine (fish, amphibians, birds, mammals), water 2. Culture: fastidious (vitamins, AA. needed) 3. Pathogenicity • E. tarda: eel • E. ictaluri: septicaemia, ascites (channel catfish)
75
GRAM NEGATIVE ANAEROBIC RODS 1. Habitat 2. Pathogenicity
``` 1. Habitat: • mucous membranes • alimentary tract, respiratory ways, genital tract, • anaerobic gut flora 2. Pathogenicity: • facultative pathogens, • mucous membranes, wounds, abscesses • mainly mixed infections ```
76
GRAM NEGATIVE ANAEROBIC RODS | 3. Genera
* Fusobacterium * Bacteroides * Dichelobacter * Provotella
77
FUSOBACTERIUM 1. Morphology 2. Staining 3. Culture
1. Morphology: polymorph, generally no flagella 2. Staining: Gram negative, irregular 3. Culture: • fastidious (protein, yeast extract), • obligate anaerobic, • CO2 • selective culture: • crystal violet, brillant green, vankomycin
78
FUSOBACTERIUM 4. Biochemistry 5. Resistance 6. Pathogenicity
``` 4. Biochemistry: • inactive, • gelatine, casein 5. Resistance: weak 6. Pathogenicity: • facultative pathogenic, • anaerobic wounds, abscesses, gangrene ```
79
FUSOBACTERIUM | 7. Species
* F. necrophorum: * F. necrophorum subsp. necrophorum * F. necrophorum subsp. funduliforme * F. nucleatum
80
F. necrophorum 1. Morphology 2. Culture 3. Biochemistry
1. Morphology: long chains, filaments 2. Culture: • blood agar, • obligate anaerobic, • haemolysis 3. Biochemistry: • carbohydrates are not utilised, • lipase, • DN-ase, • hyaluronidase
81
F. necrophorum subsp. necrophorum 1. Biochemistry 2. Pathogenicity
``` 1. Biochemistry • haemolysis, • haemagglutination, • cytotoxin, • synergism 2. Pathogenicity • calf, piglet, rabbit: necrobacillosis, • sheep: foot rot ```
82
F. necrophorum subsp. funduliforme 1. Biochemistry 2. Pathogenicity
* no haemagglutination * no cytotoxin, * no synergism * not pathogenic
83
F. nucleatum: Pathogenicity
* mainly human, dog, cat * abscess, * mastitis, metritis, * peritonitis, * wounds
84
BACTEROIDES, DICHELOBACTER, PROVOTELLA 1. Habitat 2. Morphology 3. Staining 4. Culture 5. Biochemistry
1. Habitat: alimentary tract, respiratory way, genitals, mucous membranes, water 2. Morphology: 3-5 µm rod, fimbria 3. Staining: Gram negative, irregular 4. Culture: fastidious, obligate anaerobic 5. Biochemistry: more active, proteases, keratinase (D. nodosus)
85
BACTEROIDES, DICHELOBACTER, PROVOTELLA 6. Pathogenicity 7. Species
6. Pathogenicity: facultative pathogens, necrosis, abscesses 7. Species: -B. fragilis: mainly human, gut flora, appendicitis, wounds -Dichelobacter (Bacterioides) nodosus: • fimbria, proteolysis, keratinase, • sheep foot rot -Provotella (B.) melaninogenicus: dog, cat: putrid inflammations

Bacteriology (14 decks)

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