Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Bacteriology > Lab 5 - Myobacterium, Nocardia, Actinomyces, Trueperella, Actinobaculum, Dermatophilus, Streptomyces > Flashcards

Lab 5 - Myobacterium, Nocardia, Actinomyces, Trueperella, Actinobaculum, Dermatophilus, Streptomyces Flashcards

1
Q

MYCOBACTERIUM

  1. Habitat
  2. Morphology
A
  1. Habitat:
    -infected host: obligate pathogens
    -environment: facultative pathogens, saprophytes
    • soil, water, plants
  2. Morphology:
    • rods, coccoid, branching filaments
    • cell structure: mycolic acids, lipids, waxes, surface proteins (virulence factors)
    • species differences
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

MYCOBACTERIUM

3. Staining

A

-Gram positive (but poor staining)
-Ziehl-Neelsen positive
• fuchsin – lipids
• acid and alcohol fast
-Fluorescence dyes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q 
Ziehl-Neelsen staining
A) Ziehl-Neelsen + B) Ziehl-Neelsen -
1. Fixed smear
2. Carbol-fuchsin
3. Acid and alcohol
4. Methylene blue
A
  1. Fixed smear A) colourless B) colourless
  2. Carbol-fuchsin A) red B) red
  3. Acid and alcohol A) red B) colourless
  4. Methylene blue A) red B) blue
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

MYCOBACTERIUM

4. Culture

A
  • obligate aerobic
  • broth: Henley, Sauton (cord)
  • Solid: egg, serum agar, glycerine potato
  • Selective: Petragnani (malachite green), acid and alkaline treatment
  • M.avium ssp. paratuberculosis: needs mycobactin
  • animal trial (guinea pig, rabbit, pigeon)
  • incubation time, growth characteristics (species differences)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q 
MYCOBACTERIUM
Cultural differences: M. tuberculosis
1. Incubation time
2. Shape
3. Solid medium
4. Broth
A
  1. Incubation time: 3-6 weeks
  2. Shape: slender rods
  3. Solid medium: dry colonies
  4. Broth: thin film
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q 
MYCOBACTERIUM
Cultural differences: M. bovis 
1. Incubation time
2. Shape
3. Solid medium
4. Broth
A
  1. Incubation time: 4-8 weeks
  2. Shape: short rods
  3. Solid medium: wet colonies
  4. Broth: islands
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q 
MYCOBACTERIUM
Cultural differences: M. avium subsp. avium
1. Incubation time
2. Shape
3. Solid medium
4. Broth
A
  1. Incubation time: 2-3 weeks
  2. Shape: polymorph
  3. Solid medium: cream-like colonies
  4. Broth: thick film, sediment
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q 
MYCOBACTERIUM
Cultural differences: M. avium subsp. paratuberculosis
1. Incubation time
2. Shape
3. Solid medium
4. Broth
A
  1. Incubation time: 6-12 weeks
  2. Shape: short rods
  3. Solid medium: wet colonies*
  4. Broth: thin film*
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

MYCOBACTERIUM

  1. Biochemistry
  2. Antigens
A
  1. Biochemistry: catalase -
  2. Antigens:
    -glycolipids, lipoproteins,
    • close relationship of antigens,
    • cross reactions
    • immuno-electrophoresis
    -tuberculin
    • old tuberculin: broth culture, boiled, filtered, concentrated
    • PPD (purified protein derivate): trichloracetic acid precipitation
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

MYCOBACTERIUM

  1. Resistance
  2. Pathogenicity
A
  1. Resistance: excellent
  2. Pathogenicity:
    • obligate pathogens: tuberculosis, paratuberculosis
    • facultative pathogens, saprophytes (atypical): mycobacteriosis
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Mycobacterium avium ssp. avium has cream like colonies on solid medium

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Mycobacterium are acid- and alcohol-fast bacteria

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

As a result of Ziehl-Neelsen staining, pathogenic mycobacterium are red

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Human leprosy is caused by M. lepraemurium?

A

False, by M. laprae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Mainly of mycobacteria are flagellated?

A

False, no flagella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Dermatophilus can be found on the skin of infected animals?

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Trueperella pyogenes causes exsudative dermatitis in sheep?

A

False

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Cattle lumpy jaw is caused by Actinomyces viscosus?

A

False, by Actionomyces bovis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Nocardia can be found in the infected host body only

A

False

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Mycobacteria are obligate anaerobic bacteria?

A

False, obligate aerobic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Cattle can be infected by Mycobacterium bovis only?

A

False, also other mycobacteria ssp.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

As a result of Ziehl-Neelsen staining pathogenic mycobacterium are blue?

A

False, red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Trueperella pyogenes can cause arthritis in cattle?

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Nocardia asteroides produces carotenoid pigments?

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Cattle lumpy jaw is caused by Actinomyces bovis?

A

True

26
Q

Actinobaculum suis can cause pyelonephritis in pigs?

A

True

27
Q

Trueperalle pyogenes can cause mastitis in cattle?

A

True

28
Q

Obligate pathogenic mycobacteria can multiply in the host body and the environment?

A

False, only in infected host

29
Q

Arthritis of swine can be caused by Trueperella pyogenes?

A

True

30
Q

Nocardia asteroides is an obligate anaerobic bacterium?

A

False, obligate aerobic

31
Q

Nocardia is a soil microorganism?

A

True

32
Q

Obligate pathogenic mycobacteria can multiply in the host body only?

A

True

33
Q

Mycobacterium bovis is a Gram-positive and Ziehl-Neelsen-negative?

A

False, both are positive

34
Q

Mycobacterium tuberculosis forms islands on the surface of broth?

A

False, M. bovis forms islands, M. tuberculosis forms thin film

35
Q

Animals can be infected by mycobacterium spore?

A

False, non-spore forming bacterium

36
Q

Nocardia asteroides is an obligate aerobic bacterium?

A

True

37
Q

Mycobacterium can form spore?

A

False, non-spore forming bacteria

38
Q

Pneumonia can be caused by Trueperella pyogenes?

A

True

39
Q

Nocardia asteroides is a soil bacterium?

A

True

40
Q

Incubation time of mycobacterium bovis is 6-12 weeks?

A

False, 4-8 weeks

41
Q

Dermatophilus congolensis produces carotenoid pigments?

A

False, Nocardia is producing carotenoid pigments

42
Q

Trueperella pyogenes can cause metritis?

A

True

43
Q

Pericarditis of dogs can be caused by Actinomyces hordeovulneris?

A

True

44
Q

Infectious form of Dermatophilus is the zoospore?

A

True

45
Q

Trueperella pyogenes causes mastitis or pneumonia?

A

True

46
Q

Mycobacterium is catalase negative?

A

True

47
Q

Nocardia produces carotenoid pigments?

A

True

48
Q

Dermatophilus can produce carotenoid pigments?

A

False, Norcardia produce carotenoid pigments

49
Q 
MYCOBACTERIUM
Pathogenic mycobacteria
1. M. tuberculosis 
2. M. africanum
3. M. bovis
4. M. caprae
5. M. microti
6. M. avium subsp. avium (M. avium) 
7. M. avium subsp. paratuberculosis (M. paratuberculosis)
8. M. avium subsp. silvaticum 
9. M. leprae 
10. M. lepraemurium
11. M.Pinnepedii
A
  1. M. tuberculosis: human, swine, dog, cat, parrot, cattle
  2. M. africanum: human
  3. M. bovis: cattle, ruminants, swine, dog, cat, human, BCG
  4. M. caprae: cattle, ruminants, swine, human
  5. M. microti: rodents
  6. M. avium subsp. avium: birds
  7. M. avium subsp. paratuberculosis: cattle, ruminants (paratuberculosis)
  8. M. avium subsp. silvaticum: birds (tuberculosis), ruminants (paratuberculosis)
  9. M. leprae: human (leprosy)
  10. M. lepraemurium: mouse, cat (leprosy)
  11. M.Pinnepedii: Seal
50
Q

MYCOBACTERIUM

Facultative pathogens, saprophytes (atypical)

A

(atypical)
• swine, cattle: local lesions, tuberculin reaction
• tuberculosis in fishes, reptiles: M. fortuitum, M. marinum
• groups:
1. photochromogens (need light): M. kansasii, M. marinum
2. scotochromogens (no light needed): M.scrofulaceum, M.gordonae
3. non-chromogens: M. intracellulare, M. terrae
4. rapid growers: M. phlei, M. fortuitum

51
Q

NOCARDIA

  1. Habitat
  2. Morphology
  3. Staining
A
  1. Habitat: soil
  2. Morphology: long, branched filaments
  3. Staining:
    • Gram positive
    • irregular staining,
    • slightly acid fast
52
Q

NOCARDIA

4. Culture

A
  • obligate aerobic,
  • carotenoid pigment prod. (yellow, red, pink)
  • aerial filaments
53
Q

NOCARDIA

5. Pathogenicity

A

-facultative pathogens,
-local lesions,
-N. asteroides:
•cattle: chronic suppuration, skin, subcutaneous, mastitis
•generalised nocardiosi

54
Q

Actinomycetaceae genera

A

ACTINOMYCES
TRUEPERELLA (ARCANOBACTERIUM),
ACTINOBACULUM

55
Q

Actinomycetaceae genera

  1. Habitat
  2. Morphology
  3. Staining
  4. Culture
  5. Pathogenicity
A
  1. Habitat: mucous membranes
  2. Morphology: heterogenous (rods, curved rods, filaments)
  3. Staining: Gram positive
  4. Culture:
    -Fastidious: blood agar
    -Oxygen:
    • anaerobic,
    • microaerophilic,
    • facultative anaerobic,
    -haemolysis (some species)
  5. Pathogenicity: facultative pathogens
56
Q

ACTINOMYCES

  1. Habitat
  2. Morphology
  3. Culture
  4. Species
A
  1. Habitet: mucous membr. (oral cavity, GI-tract, genitals)
  2. Morphology: rods, filaments, sulphur granules
  3. Culture: anaerobic (aerotolerant), blood agar
  4. Species:
    • A. bovis: cattle lumpy jaw
    • A.hyovaginalis: abortion, local purulent lesions
    • A.suimastitidis: pig masitis
    • A. viscosus: caryes human, dog
    • A. israeli: human actinomycosis
    • A. hordeovulneris: dog actinomycosis
57
Q

TRUEPERELLA (ARCANOBACTERIUM)

  1. Habitat
  2. Morphology
  3. Culture
  4. Species
A
  1. Habitat: mucous membr. (oropharyngeal cavity)
  2. Morphology: curved rods, coryne form
  3. Culture: blood agar, serum agar, protein demand
  4. Species:
    • T. (Arcanobacterium, Corynebacterium) pyogenes:
    •toxin (haemolysis),
    •local suppuration: mastitis, metritis, arthritis, pneumonia, navel infection
58
Q

ACTINOBACULUM

  1. Habitat
  2. Culture
  3. Species
A
  1. Habitat: preputial mucosa of boars
  2. Culture: anaerobic, blood agar
  3. Species:
    A.(Corynebacterium, Eubacterium, Actinomyces) suis:
    •anaerobic
    •pigs: genitals
    •purulent cystitis,
    •pyelonephritis
59
Q

DERMATOPHILUS

  1. Habitat
  2. Morphology
  3. Staining
  4. Culture
  5. Pathogenicity
A 
D. congolensis
1. Habitat: soil, skin
2. Morphology: coccoid/filaments
3. Staining: Gram positive
4. Culture:
-aerobic, 
-fastidious, blood agar
-propagation
•flagellated coccus (zoospora)
•filaments - branching - growth
•transverse and longitudinal divisions, - segments
•zoospora
60
Q

DERMATOPHILUS

  1. Biochemistry
  2. Pathogenicity
A 
5. Biochemistry:
• proteolytic,
• poorly saccharolytic
6. Pathogenicity:
• exudative dermatitis
• sheep, cattle, human

Bacteriology (14 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions