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Bacteriology > Lab 1 > Flashcards

Lab 1 Flashcards

1
Q

Aims of bacterial culture

A
  • Isolation of causative agents
  • AB susceptibility test
  • Vacc. prod.
  • Industry
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2
Q

Types of detection of bacteria in a sample

A
  1. Direct: smear, impression smear or PCR

2. Indirect: animal trial or inoculation of sample into a medium

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3
Q

Composition of media

A
  • Water
  • C-sources
  • N-sources
  • Vitamins and other additives
  • Osmotic pressure: 0,9% NaCl sol.
  • pH: 7.2-7.4
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4
Q

Classification of media

A

Accordring to:

  1. Origin: natural/artificial/synthetic
  2. State: liquid/semi-solid/solid
  3. Aim of culture: common (basic nutrient)/selective/differential (indicator)
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5
Q

Natural media examples

A

Potato slide, blood, serum, milk, bile, urine

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6
Q

Common media is

A

Capable of sustaining growth of bacteria

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7
Q

Selective media is

A

Contain inhibitory substances that prevent growth of unwanted bacteria sp.

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8
Q

Differential media is

A
  • Give a presumptive ID of bacteria

- Often contain sugar + pH-indicator (colour change)

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9
Q

Semi-solid medium examples

A
  • Motility tests

- Oxidation/fermentation

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10
Q

Solid medium examples

A
  • Blood agar
  • Nutrient agar
  • Mac Conkey´s agar
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11
Q

Liquid medium examples

A

-Carbohydrate broths

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12
Q

Blood agar is

A

Solid, artificial, differential medium

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13
Q

Nutrient agar is

A

Solid, artificial, common medium

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14
Q

Carbohydrate broths is

A
  • Mannitol/Dextrose/Lactose/Sucrose
  • Liquid, artificial, differential medium
  • pH-indicator: phenol-red
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15
Q

Mac Conkey´s agar is

A
  • Solid, artificial, selective, differential medium
  • Lactose + (e-coli) or - (salmonella)
  • Inhib. materials: bile salts, crystal violet
  • pH-indic: neutral red
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16
Q

Salt-Mannitol agar is

A
  • Selective
  • 10% NaCl, Mannitol, Phenol red
  • Isolation of staphylococcyl
17
Q

Chocolate agar is

A

-Heat treated (80´C, 20min) blood agar

18
Q

Chocolate agar bacterias

A
  • Avibacterium paragalinarum
  • Haemophilus parasuis
  • Taylorella equigenitalis
  • Histophilus somni
19
Q

Pure culture is

A
  • Descendants of the same bacterium

- Same geno- + phenotype

20
Q

Obligate aerobe bacteria

A
  • Grow in presence of O2 only
  • Gram+: bacillus, dermatophilus, micrococcus, nocardia, phrodococcus
  • Gram-: brucella, bordetella bronchiseptica, francicella, moraxella, pseudomonas
  • Ziehl-Neehlsen +: myobacterium
21
Q

Obligate anaerobe bacteria

A
  • Can´t tolerate O2 at all
  • Gram+: conynebacterium, erysipelothrix rhusiopathiae, listeria, staphylococcus, streptococcus
  • Gram-: actinobacillus, aeromonas, enterobacterium, haemophilus, pasteurella, vibrio
22
Q

Microaerophile bacteria

A
  • 4-6% O2 pressure, 10% CO2 pressure needed
  • Gram+: several actinomyces sp., lactobacillus
  • Gram-: campylobactes
23
Q

Capnophile bacteria

A
  • Need 5-10% CO2
  • Gram+: Actinomyces viscous, dermatophiles congolensis
  • Gram-: brucella abortus B.ovis, campylobacter jejuni C.fetus, avibacterium paragallinarum, histophilus somni, taylorella equigenitalis
24
Q

Anaerobic culture methods

A
  • Candle jar method
  • Biological O2-binding
  • Chemical: pirogallic acid+KOH/H2+palladium catalisator/Na-borohidrid+NaHCO3+ascorbic acid+H2O/other
  • Evaluation of air w. vacuum pump
  • Anaerobic broth
  • Deep agar
  • Pre-reduced media
25
Q

Light microscope

  1. Visible light
  2. Resolving power
  3. Magnification
A
  1. 400-700nm
  2. 0,2-0,4macrometer
  3. 1200-1500x
26
Q

Dark field microscope exam

A

Bacterial motility

Bacteriology (14 decks)

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