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Bacteriology > Lab 4 - Erysipelothrix, Listeria, Conyebacterium, Rhenibacterium, Rhodococcus > Flashcards

Lab 4 - Erysipelothrix, Listeria, Conyebacterium, Rhenibacterium, Rhodococcus Flashcards

1
Q

Erysipelothrix rhusiopathiae can be found in swine only

A

False (swine, sheep, birds and humans)

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2
Q

Listeria monocytogenes is catalase-neg bacterium

A

False, catalase pos.

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3
Q

Erysipelothrix rhusiopathiaeis a short thich rod

A

False, thin rods

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4
Q

Listeria monocytogenes is an alpha-haemolytic bacterium

A

False, Beta

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5
Q

Nitrate positive Carynebacterium pseudotuberculosis strains can infect sheep and goat

A

False, nitrate negative

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6
Q

Erysipelothrix rhusiopathiaeis have flagella only at room temperature

A

False, no flagella

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7
Q

Listeria monocytogenes can cause abortion in cattle

A

True

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8
Q

Erysipelothrix rhusiopathiaeis show weak beta-haemolysis on blood agar

A

False, alpha

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9
Q

Rhodococcus equi is a soil microorganism

A

True

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10
Q

Erysipelothrix rhusiopathiaeis can infect turkey

A

True

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11
Q

R colonies of Erysipelothrix rhusiopathiaeis can form sediment in broth

A

True

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12
Q

Some strains of Erysipelothrix rhusiopathiaeis causes weak beta-haemolysis on blood agar after 48 hours incubation

A

False

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13
Q

Listeria monocytogenes has flagella at low temperature (4-6 degrees) only

A

False (22 degrees)

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14
Q

Rhodococcus equi cannot produce pigments

A

False, pink pigments

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15
Q

Nitrate negative biotype strains C.pesudotuberculosis can infect horses only

A

False, sheep and goat only

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16
Q

Equine ulcerative lymphadenitis is caused by R.equi

A

False, by C.pesudotuberculosis

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17
Q

Erysipeleas of swine is caused by E.rhusiopathiae

A

True

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18
Q

Humans can be infected by Corynebacterium diphteria

A

True, mostly children

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19
Q

E.rhusiopathiae can cause septicemia in birds

A

True

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20
Q

R.colonies of E.rhusiopathiae can form uniform turbidity in broth

A

False, S colonies

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21
Q

Some strains of E.rhusiopathiae cause alpha-haemolysis on blood agar after 48 hours incubation

A

True

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22
Q

Selective medium of Listeria contains NaN3

A

False, found in E.rhusiopathiae

23
Q

Listeria monocytogenes is able to produce flagella at 37oC only

A

False (22oC)

24
Q

R.equi cannot produce capsule and pigment

A

False, can produce both

25
Pseudotuberculosis of sheep is caused by nitrate-negative strains of C.pseudotuberculosis
True
26
Corynebacterium diphteria can produce exotoxin
True, exotoxin is a toxin secreted by bacteria
27
Mainly CNS is caused by L.monocytogenes in cattle
False, in sheep
28
``` ERYSIPELOTHRIX (species?) 1. Habitat 2. Morphology 3. Staining ```
E. rhusiopathiae E. tonsillarum 1. Habitat: alimentary tract (swine), mud, slime of fishes, water 2. Morphology: 1-3 µm thin rods (S type), chains, filaments (R type) 3. Staining: Gram positive
29
ERYSIPELOTHRIX | 4. Culture
• nutrient agar, • replication 5-42 oC, pH 6.7-9.2 • blood agar: some strains α-haemolysis • S-R colonies -agar -broth: S: uniform turbidity, R: sediment • selective culture: NaN3 + crystal violet, neomycin
30
ERYSIPELOTHRIX | 5. Biochemistry
``` • catalase -, oxidase -, • fermentative • extra cellular enzymes: -neuraminidase: binding to endothelian cells -hyaluronidase: spreading ```
31
ERYSIPELOTHRIX 6. Antigens 7. Resistance
6. Antigens • two common protein antigens: protection • type specific polysaccharide haptens: 1-26: diagnostics 7. Resistance: good, halotolerance, soil
32
ERYSIPELOTHRIX | 8. Pathogenicity
• facultative pathogenic - swine: erysipelas (diamond skin disease) - sheep: wound infection, polyarthritis in lambs, post dipping lameness, valvular endocarditis - birds: septicaemia, arthritis, endocarditis (turkey, pigeon, phesant) - humans: wound infection (erysepeloid), cellulitis
33
E. tonsillarum 1. Biochem difference? 2. Serotypes? 3. Pathogenecity?
1. Biochem difference: no 2. Serotypes: serotype 7 and further 6 serotypes 3. Pathogenecity: tonsils of swine (non-pathogenic), endocarditis of dogs
34
LISTERIA 1. Habitat 2. Morphology 3. Staining
1. Habitat: soil, sewage, plants, silage, gut, food, can replicate in environment 2. Morphology: • 1-2 µm short, thick rods • flagella (22 oC) 3. Staining: Gram positive
35
LISTERIA | 4. Culture
``` • simple, • β haemolysis; weak, complete • 4-45 oC • pH 5.5-9.6 • selective: cattle serum – trypaflavin – nalidixic acid • selective enrichment: -cold enrichment: 4 oC -U-tube ```
36
LISTERIA | 5. Biochemistry
``` • catalase +, oxidase -, • fermentative • virulence factors: -listeriolysin O (haemolysin): IC replication -monocytosis factor: cell wall, lipoid ```
37
LISTERIA 6. Antigens 7. Resistance
6. Antigens: • cell wall polysaccharide • flagellar protein • 13 serotypes: most common ones: 1/2a, 4b 7. Resistance: months in soil propagation!
38
LISTERIA | 8. Pathogenicity
``` • facultative pathogenic • virulence: -haemolysis -lipolysis -monocytosis factor -cell wall lipoids • Listeriosis • sheep: mainly CNS signs • cattle: mainly abortion • rabbit: septicaemia, abortion • humans: abortion, newborn septicaemia, encephalitis • birds: septicaemia ```
39
LISTERIA | 9. Species
• L. monocytogenes: -Sheep, cattle, goat: encephalitis, abortion, septicemiae, endophtamitis -Cattle: mastitis (rare) -Dog, cat, horse: abortion, encephalitis -Pig: septicemia -Human: septicema, abortion • L. ivanovii: better haemolysis, serotype 5 -Sheep, cattle: abortion • L.innocua: sheep; meningoencephalitis • facultative intra cellular agents • saprophytic species
40
CORYNEBACTERIUM 1. Habitat 2. Morphology 3. Staining
1. Habitat: mucous membranes, skin, soil, milk (animal pathogens - saprophytes) 2. Morphology: • polymorphism, pleomorphism (coryneform), • single, palisade, Chinese letters • metachromatic granules 3. Staining: Gram positive, slightly acid-fast
41
CORYNEBACTERIUM | 4. Culture
* blood agar * weak growth on nutrient agar * B-haemolysis (sometimes with delay) * different colony morphology
42
CORYNEBACTERIUM | 5. Biochemistry
``` 5. Biochemistry: • catalase +, oxidase -, fermentative • toxin •C. diphtheriae, •C. pseudotuberculosis ```
43
CORYNEBACTERIUM 6. Antigens 7. Resistance
6. Antigens: complex | 7. Resistance: average (but C.ps. survive for months in environment)
44
CORYNEBACTERIUM | 8. Pathogenicity
* local suppuration * arthritis * metritis * mastitis
45
CORYNEBACTERIUM | 9. Species
• C. diphtheriae: exotoxin (phage) • C. pseudotuberculosis: -polymorphism, fimbria, -haemolysis, -virulence factors - exotoxin: phospholipase-D, lipoids in the cell wall, -nitrate-negative biotype: sheep, goat: pseudotuberculosis, abortion, arthritis -nitrate-positive biotype: horse; equine ulcerative lymphangitis • C. renale: fimbria, cattle (horse, swine): pyelonephritis, cystitis • C. pilosum: cattle cystitis, pyelonephritis • C. cystitidis: cattle haemorrhagic cystitis • C. bovis: • udder, milk, mastitis, abortion • C. kutscheri: • rodents
46
``` RHODOCOCCUS (species) 1. Habitat 2. Morphology 3. Staining ```
R. equi 1. Habitat: soil, plant, gut (horse) 2. Morphology: coccus-rod cycle, capsule 3. Staining: Gram-positive, some acid-fast
47
RHODOCOCCUS | 4. Culture
* simple (nutrient agar, nutrient broth, 48h) * obligate aerob * pigment (pink) * capsule (polysaccharide): mucous colonies
48
RHODOCOCCUS | 5. Biochemistry
- Catalase +, oxidase - - Neg. in OF-test - CAMP test
49
RHODOCOCCUS | 6. Antigens
10 serotypes (on basis of capsule polysaccharide)
50
RHODOCOCCUS | 5. Pathogenicity
* Foal (most seceptible): pneumonia, ulcerative enteritis, arthritis * Cattle, swine: mesentheric lymph nodes, abscesses * Human: pneumonia, abcess-formation (immunosuppression, AIDS)
51
RENIBACTERIUM 1. Habitat 2. Morphology 3. Staining
Renibacterium salmoninarum 1. Habitat: water, fish 2. Morphology: 1-1.5 µm rods, short chains 3. Staining: Gram positive
52
RENIBACTERIUM | 4. Culture
- Aerobic - 15-18 oC - Fastidious (cystein, serum)
53
RENIBACTERIUM | 5. Pathogenicity
* obligate pathogenic, intra cellular | * salmon, trout: abscesses in kidneys and other organs

Bacteriology (14 decks)

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