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Bacteriology > Lab 4 - Erysipelothrix, Listeria, Conyebacterium, Rhenibacterium, Rhodococcus > Flashcards

Lab 4 - Erysipelothrix, Listeria, Conyebacterium, Rhenibacterium, Rhodococcus Flashcards

1
Q

Erysipelothrix rhusiopathiae can be found in swine only

A

False (swine, sheep, birds and humans)

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2
Q

Listeria monocytogenes is catalase-neg bacterium

A

False, catalase pos.

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3
Q

Erysipelothrix rhusiopathiaeis a short thich rod

A

False, thin rods

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4
Q

Listeria monocytogenes is an alpha-haemolytic bacterium

A

False, Beta

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5
Q

Nitrate positive Carynebacterium pseudotuberculosis strains can infect sheep and goat

A

False, nitrate negative

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6
Q

Erysipelothrix rhusiopathiaeis have flagella only at room temperature

A

False, no flagella

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7
Q

Listeria monocytogenes can cause abortion in cattle

A

True

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8
Q

Erysipelothrix rhusiopathiaeis show weak beta-haemolysis on blood agar

A

False, alpha

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9
Q

Rhodococcus equi is a soil microorganism

A

True

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10
Q

Erysipelothrix rhusiopathiaeis can infect turkey

A

True

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11
Q

R colonies of Erysipelothrix rhusiopathiaeis can form sediment in broth

A

True

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12
Q

Some strains of Erysipelothrix rhusiopathiaeis causes weak beta-haemolysis on blood agar after 48 hours incubation

A

False

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13
Q

Listeria monocytogenes has flagella at low temperature (4-6 degrees) only

A

False (22 degrees)

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14
Q

Rhodococcus equi cannot produce pigments

A

False, pink pigments

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15
Q

Nitrate negative biotype strains C.pesudotuberculosis can infect horses only

A

False, sheep and goat only

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16
Q

Equine ulcerative lymphadenitis is caused by R.equi

A

False, by C.pesudotuberculosis

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17
Q

Erysipeleas of swine is caused by E.rhusiopathiae

A

True

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18
Q

Humans can be infected by Corynebacterium diphteria

A

True, mostly children

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19
Q

E.rhusiopathiae can cause septicemia in birds

A

True

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20
Q

R.colonies of E.rhusiopathiae can form uniform turbidity in broth

A

False, S colonies

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21
Q

Some strains of E.rhusiopathiae cause alpha-haemolysis on blood agar after 48 hours incubation

A

True

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22
Q

Selective medium of Listeria contains NaN3

A

False, found in E.rhusiopathiae

23
Q

Listeria monocytogenes is able to produce flagella at 37oC only

A

False (22oC)

24
Q

R.equi cannot produce capsule and pigment

A

False, can produce both

25
Q

Pseudotuberculosis of sheep is caused by nitrate-negative strains of C.pseudotuberculosis

A

True

26
Q

Corynebacterium diphteria can produce exotoxin

A

True, exotoxin is a toxin secreted by bacteria

27
Q

Mainly CNS is caused by L.monocytogenes in cattle

A

False, in sheep

28
Q 
ERYSIPELOTHRIX
(species?)
1. Habitat
2. Morphology
3. Staining
A

E. rhusiopathiae
E. tonsillarum
1. Habitat: alimentary tract (swine), mud, slime of fishes, water
2. Morphology: 1-3 µm thin rods (S type), chains, filaments (R type)
3. Staining: Gram positive

29
Q

ERYSIPELOTHRIX

4. Culture

A

• nutrient agar,
• replication 5-42 oC, pH 6.7-9.2
• blood agar: some strains α-haemolysis
• S-R colonies
-agar
-broth: S: uniform turbidity, R: sediment
• selective culture: NaN3 + crystal violet, neomycin

30
Q

ERYSIPELOTHRIX

5. Biochemistry

A 
• catalase -, oxidase -,
• fermentative
• extra cellular enzymes:
 -neuraminidase: binding to endothelian cells
 -hyaluronidase: spreading
31
Q

ERYSIPELOTHRIX

  1. Antigens
  2. Resistance
A
  1. Antigens
    • two common protein antigens: protection
    • type specific polysaccharide haptens: 1-26: diagnostics
  2. Resistance: good, halotolerance, soil
32
Q

ERYSIPELOTHRIX

8. Pathogenicity

A

• facultative pathogenic

  • swine: erysipelas (diamond skin disease)
  • sheep: wound infection, polyarthritis in lambs, post dipping lameness, valvular endocarditis
  • birds: septicaemia, arthritis, endocarditis (turkey, pigeon, phesant)
  • humans: wound infection (erysepeloid), cellulitis
33
Q

E. tonsillarum

  1. Biochem difference?
  2. Serotypes?
  3. Pathogenecity?
A
  1. Biochem difference: no
  2. Serotypes: serotype 7 and further 6 serotypes
  3. Pathogenecity: tonsils of swine (non-pathogenic), endocarditis of dogs
34
Q

LISTERIA

  1. Habitat
  2. Morphology
  3. Staining
A
  1. Habitat: soil, sewage, plants, silage, gut, food, can replicate in environment
  2. Morphology:
    • 1-2 µm short, thick rods
    • flagella (22 oC)
  3. Staining: Gram positive
35
Q

LISTERIA

4. Culture

A 
• simple,
• β haemolysis; weak, complete
• 4-45 oC
• pH 5.5-9.6
• selective: cattle serum – trypaflavin – nalidixic acid
• selective enrichment:
 -cold enrichment: 4 oC
 -U-tube
36
Q

LISTERIA

5. Biochemistry

A 
• catalase +, oxidase -,
• fermentative
• virulence factors:
 -listeriolysin O (haemolysin): IC replication
 -monocytosis factor: cell wall, lipoid
37
Q

LISTERIA

  1. Antigens
  2. Resistance
A
  1. Antigens:
    • cell wall polysaccharide
    • flagellar protein
    • 13 serotypes: most common ones: 1/2a, 4b
  2. Resistance: months in soil propagation!
38
Q

LISTERIA

8. Pathogenicity

A 
• facultative pathogenic
• virulence:
 -haemolysis
 -lipolysis
 -monocytosis factor
 -cell wall lipoids
• Listeriosis
• sheep: mainly CNS signs
• cattle: mainly abortion
• rabbit: septicaemia, abortion
• humans: abortion, newborn septicaemia, encephalitis
• birds: septicaemia
39
Q

LISTERIA

9. Species

A

• L. monocytogenes:
-Sheep, cattle, goat: encephalitis, abortion, septicemiae, endophtamitis
-Cattle: mastitis (rare)
-Dog, cat, horse: abortion, encephalitis
-Pig: septicemia
-Human: septicema, abortion
• L. ivanovii: better haemolysis, serotype 5
-Sheep, cattle: abortion
• L.innocua: sheep; meningoencephalitis
• facultative intra cellular agents
• saprophytic species

40
Q

CORYNEBACTERIUM

  1. Habitat
  2. Morphology
  3. Staining
A
  1. Habitat: mucous membranes, skin, soil, milk (animal pathogens - saprophytes)
  2. Morphology:
    • polymorphism, pleomorphism (coryneform),
    • single, palisade, Chinese letters
    • metachromatic granules
  3. Staining: Gram positive, slightly acid-fast
41
Q

CORYNEBACTERIUM

4. Culture

A
  • blood agar
  • weak growth on nutrient agar
  • B-haemolysis (sometimes with delay)
  • different colony morphology
42
Q

CORYNEBACTERIUM

5. Biochemistry

A 
5. Biochemistry:
• catalase +, oxidase -, fermentative
• toxin
•C. diphtheriae,
•C. pseudotuberculosis
43
Q

CORYNEBACTERIUM

  1. Antigens
  2. Resistance
A
  1. Antigens: complex

7. Resistance: average (but C.ps. survive for months in environment)

44
Q

CORYNEBACTERIUM

8. Pathogenicity

A
  • local suppuration
  • arthritis
  • metritis
  • mastitis
45
Q

CORYNEBACTERIUM

9. Species

A

• C. diphtheriae: exotoxin (phage)
• C. pseudotuberculosis:
-polymorphism, fimbria,
-haemolysis,
-virulence factors
- exotoxin: phospholipase-D, lipoids in the cell wall,
-nitrate-negative biotype: sheep, goat: pseudotuberculosis, abortion, arthritis
-nitrate-positive biotype: horse; equine ulcerative
lymphangitis
• C. renale: fimbria, cattle (horse, swine): pyelonephritis, cystitis
• C. pilosum: cattle cystitis, pyelonephritis
• C. cystitidis: cattle haemorrhagic cystitis
• C. bovis:
• udder, milk, mastitis, abortion
• C. kutscheri:
• rodents

46
Q 
RHODOCOCCUS 
(species)
1. Habitat
2. Morphology
3. Staining
A

R. equi

  1. Habitat: soil, plant, gut (horse)
  2. Morphology: coccus-rod cycle, capsule
  3. Staining: Gram-positive, some acid-fast
47
Q

RHODOCOCCUS

4. Culture

A
  • simple (nutrient agar, nutrient broth, 48h)
  • obligate aerob
  • pigment (pink)
  • capsule (polysaccharide): mucous colonies
48
Q

RHODOCOCCUS

5. Biochemistry

A
  • Catalase +, oxidase -
  • Neg. in OF-test
  • CAMP test
49
Q

RHODOCOCCUS

6. Antigens

A

10 serotypes (on basis of capsule polysaccharide)

50
Q

RHODOCOCCUS

5. Pathogenicity

A
  • Foal (most seceptible): pneumonia, ulcerative enteritis, arthritis
  • Cattle, swine: mesentheric lymph nodes, abscesses
  • Human: pneumonia, abcess-formation (immunosuppression, AIDS)
51
Q

RENIBACTERIUM

  1. Habitat
  2. Morphology
  3. Staining
A

Renibacterium salmoninarum

  1. Habitat: water, fish
  2. Morphology: 1-1.5 µm rods, short chains
  3. Staining: Gram positive
52
Q

RENIBACTERIUM

4. Culture

A
  • Aerobic
  • 15-18 oC
  • Fastidious (cystein, serum)
53
Q

RENIBACTERIUM

5. Pathogenicity

A
  • obligate pathogenic, intra cellular

* salmon, trout: abscesses in kidneys and other organs

Bacteriology (14 decks)

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