Lab Flashcards
What relevant legislation is there for laboratory diagnostics?
Health and safety at work act 1974
COSHH
environmental protection act 1990
The control of pollution and disposal reg 1988
The collection and disposal of waste regulations 1988
The hazardous waste reg 2005
RIDDOR
Health and safety reg 1981 (first aid)
The electrical equipment (safety) reg 1994
Which part of the microscope contains the ocular lenses that magnify the primary image formed by a the objective lens?
Eye piece
On a microscope which bit rotates and holds the objective lens?
Nose piece
There are normally 4 of these on a microscope, each with different magnification ?
Objective lens
A platform on the microscope that holds the slide, can move, has a hole in the centre that allows light through?
Stage
Allows specific points of interest to be recorded on a microscope.
Vernier scale
On a microscope this condenses light from the light source on to the specimen.
Substage condenser
Controls the amount of light coming through the substage condenser on a microscope?
Iris diagram
Raises or lowers the stage and allows the image to be focused on a microscope?
Focus knobs
Alters the level of light produced by the light source on a microscope?
Rheostat
Explain the battlement technique?
It’s the methodical manor of viewing a slide
—>- | | —>—l |—->
what are the two types of centrifuges?
Angle head - commonly used
Swing out head
What is a appropriate centrifuge speed for blood?
10,000 rpm for 5 minutes?
What is a appropriate centrifuge speed for urine?
2000rpm for 5 minutes
Quality control or quality assurance
Refers to the measure that must be included during each assay run, in order to verify that test working properly
Quality control
Quality control or quality assurance
Is defined as the overall programme which ensures that the final results reported by the lab are correct.
Quality assurance
Quality control or quality assurance
Which one should be carried out:
- at the beginning of the day
- after an analyser is serviced
- when reagent lots are charged
- after calibration
- when patient results seem inappropriate
- as indicated by the manufacturer
Quality control
Why should you use the smaller syringe possible when taking bloods ?
To avoid rupturing the RBC
Why should you used the needle with the largest gauge possible when taking bloods?
To avoid rupturing the rbc
Which anticoagulant is not suitable for birds or reptiles?
EDTA
What anticoagulant would you place a glucose sample in? What colour tube and vacutainer is it?
Fluoride/oxalate
Tube- yellow
Vacutainer - grey
Leishmans, giemsa, diff quick are what type of stain?
Romonowsky stain