L9 - Addiction Flashcards
Opiate receptors
u opiate
nicotine receptors
acetylcholine
cannabis receptors
cannaboid
alcohol receptors
several including; - GABA and NDMA
secondary target of drugs
mesolimbic system - dopamine
dopamine release
ventral tegmental area neurons
mesolimbic pathway
natural rewards from sex, food, social interaction
Dopamine action on
dopamine and serotonin pathways
Drug addiction contributing factors
genetics
social environment
personality and personal history
sensitivity
inability to tolerate the adverse effects of a drug
tolerance
reduction in sensitivity when drug is used repeatedly
model organism for drug addiction
drosophila
human disease suited for analysis in drosophila
cancer
developmental, metabolic and neurological disorders,
cardiovascular disease
drug receptors lacking in flies
opioid
cannaboid
experimental approaches to measure ethanol effects on flies
booz-o-mat
inebriometer
movement of flies exposed to ethanol
immediate phase - increased locomotion
over time - slow down until sedation
elution of flies exposed to ethanol
fast
ethanol sensitivity on alcoholism
low sensitivity increases future risk of alcoholism
technique to identify genes implicated in sensitivity
induce mutations, measure elution times,
identify fastest flies
cheapdate mutant
increased ethanol sensitivity
gene mutated in cheapdate
allele of amnesiac
amnesiac
neuropeptide
amnesiac function
activates cAMP pathway
mutation in cheapdate
reduces downstream transcription due to inactivation of CREB
cAMP activity
decreases alcohol sensitivity
technique to measure tolerance
repeat inebriometer test,
tolerance indicated by longer elution time
hangover
encodes zn-finger - acts as TF
hangover effect on cAMP
speeds up cAMP inactivation
what promotes development of tolerance
faster deactivation of cAMP pathway
chronic alcohol exposure
increases HPA activity - elevated glucocorticoid levels
