L8 - Stress

Question 1

Cortico-releasing neurons location

Answer 1

PVN

Question 2

progenitors repressed by shh give rise to

Answer 2

PVN neurons

Question 3

Neurons involved in stress pathway

Answer 3

corticotropin-releasing hormone neurons, POMC neurons, Steroidogenic factor neurons.

Question 4

ACTH stimulates

Answer 4

cortisol release

Question 5

negative feedback loops

Answer 5

maintain homeostasis

Question 6

DISC1

Answer 6

genetic risk factor for mental illness

Question 7

model organism for behavioural studies

Answer 7

zebrafish

Question 8

why are zebrafish used for behavioural studies

Answer 8

statistical analysis

Question 9

DISC1 mRNA & protein expression

Answer 9

developing hypothalamus

Question 10

How was DISC1 expression identified

Answer 10

in situ hybridisation

Question 11

DISC1 expression overlap

Answer 11

FGF+ and Shh+ hypothalamic progenitor cells

Question 12

Disc1 mutation -hypothalamic progenitors

Answer 12

abnormal behaviour,

differentiate prematurely

Question 13

effect of disc1 mutant on progenitor pool

Answer 13

depletes progenitor pool

Question 14

what neurons are reduced in disc1 mutants

Answer 14

POMC neurons

Question 15

what neurons are over-expressed in disc1 mutants

Answer 15

Sf1+ and Crh+

Question 16

Loss of DISC1 protein leads to

Answer 16

abnormal differentiation pattern of neurons involved in stress regulation

Question 17

measurement of abnormal stress response

Answer 17

behavioural assays

neuroendocrine function

Question 18

disc1 mutant fish - stress response

Answer 18

no stress response to schreckstoff,

no elevation in cortisol