L6 Bacterial Persistence at the Aerobic-Anaerobic interface Flashcards

1
Q

define aerobic bacteria

A

prefer oxic environments

21% O2, atmospheric

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2
Q

define microaerobic

A
hypooxic environments 
trace O2 (2-10%)
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3
Q

define anaerobic

A

anoxic environments
strictly 0%
facultative (can live in both)

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4
Q

what is eutrophication

A

dead zones (environmental hypoxia)

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5
Q

types of bacteria environments and oxygen status

A

host associated: internal or bacteria dense are anaerobic

soils and oceans: decreasing O2 with increasing depth

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6
Q

what feature do bacteria have that enables to be live in a diverse range of environments

A

can have multiple electron donors and accepters
fermentation is sustainable for long periods of time
FLEXIABLE

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7
Q

why can bacteria live on so many surfaces

A

diverse range of digestive enzymes for breaking down polymers

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8
Q

how does bacteria break down polymers from the environment

A

enzymes produced inside the cell are transported to the surface where they break down polymers intro monomers and oligomers for the conserved intracellular metabolic pathways

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9
Q

what is bacterial persistence

A

when bacteria are growing slowly or not at all
not dying, still metabolically active
not going through lag or log phase

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10
Q

is bacterial true dormancy

A

no, endospores would be an example of true dormancy

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11
Q

what is an r-type strategy

A

fast growing
use nutrients they don’t need
bust and boom
dynamic persistence of small subpopulation

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12
Q

what is a k-type strategist

A

uses food efficiently
slow growing
bulk population persists
less drastic metabolic changes under stress

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13
Q

example of r-type strategists

A

E. Coli

B. subtilis

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14
Q

example of K-type strategist

A

M. tuberculosis

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15
Q

define antibiotic resistance

A

Heritable

genetic changes that allow bacteria to grow

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16
Q

define antibiotic persistence

A

Non-heritable
physiological changes
non-growing or slow growing bacteria

17
Q

what is the relationship between resistance and persistence

A

persistence can lead to resistance

18
Q

what does persistence cause?

A

a shift in energy metabolism that permits survival without growth

19
Q

features of environmental persistence

A

response to fluctuating food supplies
different population level strategies (r/k)
adaptation for long-tern survival
more time to respond

20
Q

what is hypoxia

A

depletion of oxygen

21
Q

what does hypoxia trigger in bacteria like TB

A

stationary phase/slowed growth

persistent phenotypes

22
Q

what intracellular environements does hypoxia occur for TB

A

macrophage phagolysosomes

granuloma: immune structures forming wall