L10 Bacterial ATP synthase

Question 1

how much ATP is consumed in a regular day

Answer 1

65kgs

Question 2

how much ATP is in the body at any one time

Answer 2

100g

Question 3

how much O2 is used to produced 65kg of ATP

Answer 3

380L

Question 4

who discovered F1-F0 ATP synthase

Answer 4

Efraim Racker

Question 5

what are the fractions of ATP synthase

Answer 5

F1: soluble fraction (hydrolytic activity)
F0: sensitive to oligomycin

Question 6

how the are the ATP synthase fractions connected

Answer 6

prereferral stalk

Question 7

where is the F1 fraction found

Answer 7

cytoplasm

Question 8

where is the f2 fraction found

Answer 8

membrane bound

Question 9

what happens during ATP synthesis

Answer 9

proton enters a single half channel in the a subunit and docks to the c subunit.

• proton goes around the c subunit and out through the other half channel of the a subunit into the cytoplasm
• as the c subunit rotates with protons it drives the change in conformation of the alpha and beta subunits

Question 10

What is the functions of ATP synthase

Answer 10

synthesise ATP

hydrolyse ATP

Question 11

what does the F1 motor consist of

Answer 11

3 alpha subunits + 3 beta subunits = catalytic head

Question 12

how is the F1 motor connected to the prereferral stalk

Answer 12

delta subunit

Question 13

what does the prereferral stalk consist of

Answer 13

delta subunit
1 a subunit
2 b subunits

Question 14

how many c subunits are in the rotor component in mitochondria

Answer 14

8

Question 15

how many c subunits are in the rotor component in bacteria

Answer 15

9-15

Question 16

what is the gamma subunit

Answer 16

central stalk that

Question 17

where is the epsilon subunit found

Answer 17

connected to the gamma subunits

Question 18

what does the rotary element of ATP synthase consist of

Answer 18

c-subunits
gamma subunit
epsilon subunit

Question 19

describe the A subunit of ATP synthase

Answer 19

extremely hydrophobic with hydrophilic channels

Question 20

how many revolutions per second does the c subunit do

Answer 20

134

Question 21

how many molecules of ATP are produced in one rotation of the C subunit

Answer 21

3

Question 22

what subunits is ATP synthesised on

Answer 22

beta

Question 23

when the conc of the proton motive force gets low what can bacteria do

Answer 23

hydrolyse ATP

Question 24

what happens when ATP is hydrolyses

Answer 24

c subunit rotates in opposite direction and protons move from the cytoplasm to the outside of the cell

Question 25

which motor is responsible for synthesis and which is for hydrolysis

Answer 25

F1: hydrolysis
FO: synthesis

Question 26

what were the three conformations of the beta subunits

Answer 26

beta e: empty
beta dp: ADP bound
beta tp: ATP bound

Question 27

where does the gamma subunit sit

Answer 27

in the middle of the catalytic head, connected to the beta subunits

Question 28

what happens when the gamma subunit rotates

Answer 28

the beta subunits change conformation

Question 29

how was the rotational hypothesis proven

Answer 29

tethered ATP synthase upside to glass and attached the gamma subunit to a fluorescent actin filament rather than the c subunit.

looked at hydrolysis of ATP to ADP

Question 30

how does ATP synthase rotate

Answer 30

120 degrees then pause
x 3
within 120 degrees, there were 90 degree (ATP binding) and 30-degree rotation (ADP release)