L10 virus cultivation and identification Flashcards
1
Q
Specimens used to culture viruses
A
- Throat swabs
- Nasopharyngeal aspirates
- Vesicle fluid
- Blood
- Cerebrospinal fluid
- Salive
- Biopsy (skin, organs)
2
Q
Ideas around transporting viruses
A
- highly regulated
- Cold conditions
3
Q
Direct viral detection methods
A
- Electron microspy (UC)
- Isolation of infectious virus by cell culture (UC)
- Detection of viral genome by molecular methods (C,PCR)
- Detection of virions by serological methods (C) - looks for IF proteins
UC= uncommon
4
Q
Indirect detection of viruses [def]
A
measurement of virus specific hot immune response after infection
5
Q
[def] direct detection of viruses
A
Measurement of virion
6
Q
Electron microscopy
A
- image of virus but not really identified
- Doesn’t need organsims specific reagents
7
Q
Scanning EM can add information on external aspects of a virion.
A
8
Q
Viral isolation
A
- Isolated from infected tissue by propagation in an animal or human cell, egges or lab-animals
- Not replicate outside a living cell.
- First culture->microbial contaminations
9
Q
Cell culture
A
- Common propagation method
- Human/animal/plant tissue are dissociated into single cells by mechanical destuption (smashed) or proteolytic enzymes.
- Cells suspended in medium and placed into a culture vessel.
- Some of the cells will grow in the suspension and form a monolayer.
- The cell culture medium is an isotonic solution of salts, glucose, vitamins and amino acids with 7.3pH and factors.
10
Q
Three types of cell cultures
A
- Primary cc from animal/human, short (5-10) cell division. From monkey kidney or human foreskin.
- Diploid cells- single cell types and divides up to 100 times and retains diploid chromo number. WI-38 common celline.
- Continuous celline has single cell and can propagate indefinitily, from tumors or treatment by mutagen. Vero cells (ex)
11
Q
Types of cell lines
A
- primary
- Mouse fibroblast
- human epithethial cell line
