Introduction To Histology Flashcards
What is the most commonly used dye combination
Haematoxylin and Eosin (H&E)
What colour does haematoxylin and eosin stain cytoplasm and nuclei
Nuclei stain blue and cytoplasm stains pink
What also stains pink when using a H&E stain
Most extra-cellular fibres such as collagen and elastic stain pink with H&E stain
What is PAS used to stain
Sugars
What is van Gieson used to stain
Elastic
What is Alcian blue used to stain
Mucins
What is trichromatic stain used for
It is used to stain 3 types of cells to visualise the difference between them
What is an example of a small cell
A lymphocyte which is about 10 micrometers in diameter
What is an example of a large cell
A motor neuron which is about 100 micrometers wide with axons up to 1 meter in length
What shape are these cells
Rounded
What shape are these cells
Polygonal
What shape are these cells
Fusiform
What shape are these cells
Columnar
What shape are these cells
Cuboidal
What shape are these cells
Squamous
In general what is the difference between metabolically active and inactive cells
Metabolically active cells are larger than metabolically inactive cells
Metabolically active cells often have nucleoli
What is the life span of tissues lining the gut
Days
What is the life span of tissues such as blood, skin and connective tissues
Months
What is the lifespan of bones and tendons
Years
What is the life span of skeletal muscle
Nearly your whole life
What is the life span of nerves, brain, cardiac muscle and germ cells
They last your whole life
What are the arrows pointing at
Nucleolus
What is this showing
Mitochondria
What is this showing
Rough endoplasmic reticulum
What is this showing
Smooth endoplasmic reticulum
What is this showing
Golgi apparatus
What is this showing
Lysosomes
How big are microfilaments and what is an example
Microfilaments are about 5 micrometers
Actin is an example of a Microfilament
How big are microtubules and what is an example
Microtubules are 25 micrometers
Tubulin is an example
What calls don’t have any microtubules in
Erythrocytes
How big are intermediate filaments
10 diameters
Where are the intermediate filaments cytokeratins found
Epithelial cells
Where are the intermediate filament desmin found
Myocytes
Where are the intermediate filaments glial fibrillary acidic proteins found
Astrocytic glial cells
Where are the intermediate filaments neurofilament proteins found
Neurons
Where are the intermediate filaments nuclear laminin found
Nuclei of all cells
Where are the vimentin intermediate filaments found
Mesodermal cells
What are the different types of tissues
Epithelial tissue
Muscle tissue
Supporting tissue
Nerve tissue
Germ cell tissue
