inintro lecture Flashcards
what is in the basic metabolic panel
- glucose
- albumin
- total protein
- sodium
- potassium
- chloride
- blood urea nitrogen
- creatinine
- CO2
what is in the complete metabolic panel a combination of
combo of basic and liver panel
what gets tested in the CMP
basic panel and liver
- ALT
- AST
- ALP
- total biliruben
what is in a lipid profile
- triglycerides
- cholesterol
- HDL
- LDL
how does fasting effect lipid panel
affects glucose and triglycerides
what is the matrix of choice
- plasma or serum
- urine
- feces
- other body fluids
what do photometric instruments measure
light intesity without consideration of wavelengthd
define beers law
concentration of a substance is directly proportional to the right amount of light absorbed
what do spectrophotometric instruments measure
light transmitted by a soln to determine concentration of light absorbing substance in system
what is the electromagnetic specturm
protons of energy travelling in a wave where one wavelength is a complete wave cycle
what are the two kinds of light sources used
- tungston: most common, used for visible and infrared
- deuterium: used for UV
what is the order of mechanisms used on light in a spectrophotometer
- light source
- entrance slit
- monochromator
- exit slit
- sample cuvette
- detector
what is percent transmittance
T/I *100
T= radiant energy transmitted
I= incident radiant energy
what does high transmittance and low transmittence correlate to
high T = clearer, more light transmitted through sample
lower T = less light through sample
what are the two kinds of photodetectors
photo cell - less sensitive
photo tube - external power supply
