Inhibitors of Microbial Protein Synthesis I

Question 1

Protein Inhibitors that act against 50s and 70s ribosomal RNA

Answer 1

Chloramphenicol, macrolides, oxalinones, lincosamides,and streptogramins

Question 2

Protein Inhibitors that act on 30s ribosomal RNA

Answer 2

tetracyclines, aminoflycosides, aminocyclitols

Question 3

Broad spectrum protein inhibitors

Answer 3

chloramphenicol, and tetracyclines

Question 4

Moderate Spectrum protein inhibitors

Answer 4

Macrolides, and aminoglycosides

Question 5

Narrow Spectrum Protein Inhibitors

Answer 5

Lincosamides, Streptogramins, Oxazoladinones, daptomycin (cyclic lipopeptides), spectinomycin (aminocyclitols)

Question 6

Narrow Spectrum Protein Inhibitors

Answer 6

Lincosamides, Streptogramins, Oxazoladinones, Aminocyclitols, and Daptomycin

Question 7

Chloramphenicol Mechanism of Action

Answer 7

Stops transpeptidation by blocking binding of aminoacyl on charged tRNA to acceptor site on ribosome-mRNA complex.

Question 8

Chloramphenicol Adverse reactions

Answer 8

GI irritation, superinfections, suppression of RBC production, aplastic anemia, infant cyanosis (deficient GT)

Question 9

What Protein Inhibitor is used for resistant gonorrhea

Answer 9

Aminocyclitols

Question 10

Chloramphenicol Interactions

Answer 10

Inhibits hepatic metabolism and antagonizes bacterocidal drugs

Question 11

Chloramphenicol resistance

Answer 11

acetyltransferases that inactivate drug

Question 12

Clinical Uses of Chloramphenicol

Answer 12

bacteriostatic, limited by toxicity, back up for severe salmonella, pneumococcal and meningococcal meningitis, topical for eye infections

Question 13

Tetracyclines

Answer 13

tetracycline, demaclocycline, doxycycline, minocycline, chlortetracycline, oxytetracycline, methacycline

Question 14

Tetracycline mechanism of action

Answer 14

reversibly bind 30s and block amino acid charged tRNA from binding acceptor site

Question 15

Tetracycline Resistance

Answer 15

plasmid mediated, 3 mechanisms: impaired influx, proteins that decrease access, and enzymatic deactivation