In Ovo Techonology Flashcards
5 Principles of Success in Ovo (LPSIS)
Egg Shell Location
Shell Penetration
Sanitation
Site of Injection
Vaccine Specifications
Critical Aspects of Vaccine Machine
Needle Sanitation
Vaccine Type
Concentration
Aseptic Handling
Critical Aspects of Egg during In Ovo Vaccination
Egg Location
Shell Penetration
Critical Aspects of Embryo during In Ovo Vaccination
Site of Injection
Embryo Age
Locator cup function
Positions egg
Punch function
Egg shell penetration
Needle is located inside of _
Punch
Method Improving Sanitation During Shell Penetration
Needle inside of punch permits targeted, efficient sanitation of contact points of injection mechanism
Method to reduce egg-to-egg bacterial carry over
Sanitation between injections
Contaminants of Vaccine Thaw Bath (HHTDC)
Hands, Hygiene, Tap, Distilled, Chlorine
Vaccine Prep
Use only for vaccine prep
Sanitize weekly
Clean air supply
Injection Process Improvements
Locating
Punch Speed
Reduced punch & needle stress
Egg support
Egg Handling Improvements
Rubber Suction Cups
Haylo
Hatching basket sanitation
Flexible wire in Haylo is made of
Nitinol - Nickle Titanium
Hatching Basket Sanitation Process
Clean, Disinfect, Dry
Egg Compartments
Air Cell, Allantois, Amnion, Embryo, Yolk Sac
Position to hatch
Head under right wing
Stalk of yolk sac enters
Abdomen
Optimal Timing for In Ovo Vaccination
17d 12h - 19d 4h
Acceptable Injection Sites
Amnion, Embryo, IM
Unacceptable Injection Sites (Too Shallow)
Air Cell, Allantois
As length of incubation increases so does the use of _ as the injection site
Embryo
As length of incubation decreases, the use of _ as site of injection increases
Amnion
in Ovo Vaccine Specifications
Vaccine Type
Vaccine Concentration
Vaccine Compatibility
Embryonic Age at injection
Site of injection, sanitation
Onset and duration of immunity
Disease challenge
Maternal Antibodies
Immunological tolerance can be induced at - d
8-14
Experimental In Ovo Vaccination Window
16-19d
Commercial In Ovo Vaccination Window
17d (12h) -19d (4h)
In Ovo Stimulation uses _, _, _ on day _
Aims to stimulate _ of _ and _ _
Site of Injection: ____
Volume: ___
Prebiotics, Synbiotics, Betadine, 12
Colonization, gut, gut development
Air Sac
0.2ml
In Ovo Feeding: Administration of _ into _ to enhance egg _ and _ performance on _-_d, has _ effect
Nutrients, Amnion, Nutrition, Growth, 17-18d, epigenetic
Gallipro Hatch uses _ _ to improve _
Enterococcus faecium, FCR
2 Types of probiotics used In Ovo Feeding
Enterococcus Faecium
Bacillus Subtitles
In Ovo Feeding Challenges
Volume
Embryonic Age
Type & Concentration
Area of Injection
Bacteria Load built up over time and embryo safety
5 In Ovo Feeding Promises
Reduce mortality
Increase FCR
Increase Immune Response
Reduced Incidence of Skeletal Disorders
Increased muscle and breast-meat yield
In Ovo so far some solutions _ affect hatchability, _ improvements limited to early age, performance benefits but don’t last until _ age
Negatively, BW, Market