Immunology 1 Flashcards

1
Q

Hematopoietic cells

A

Stem cells that produce;

myeloid stem cells (makes basophils, eosinophils, neutrophils)

and

lymphoid stem cells (mature into T and B cells)

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2
Q

Neutrophils (role and characterisitcs)

A

Innate Immune

Also called polymorphonuclear cells (PMNs) bc their segmented nucleus

Increase in number in response to infection and inflammation (first cells to arrive)

Major line of defense against pus-forming bacteria

Phagocytes - engulf bacteria and produce debris

Granulocytes - have enzymes and proteins that kill invading pathogens or threats

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3
Q

Monocytes and MAcrophages

A

Innate and Adaptive (macro) Immune

long lived cell with bilobed nucleus, originate in bone marrow

In response to infection and inflammation they migrate to the tissue and mature into macrophages

They take over neutrophils and filter the debris they made, kill (phagocytosis) any damaged bacteria or bacteria that was too large for neutrophils

Also eliminate old RBC or dead neutrophils

(They ingest bacteria and use it to express as an antigen on their MHC to signal the T helper cells to respond)

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4
Q

Eosinophils

A

Innate

Involved in allergic responses and parasitic infections

When invading organisms are too large for neutrophils and macrophages to eliminate, eosinophils surround the pathogen and release the contents of their granules to induce membrane damage and death

granule contents:

histamine: vasodilator
heparin: anticoagulant
cytokines: inflammatory reactions

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5
Q

Basophils and Mast cells

A

Innate

Both located close to blood vessels and have similar fxn

Basophils - major role in allergic reactions releases substances from their granules

Mast cells -
- involve in allergic reactions, anaphylaxis, wound healing, and against pathogens
- possess granules containing histamine that dilate blood vessels

Both increase blood supply which recruits more phagocytes to the infected site

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6
Q

Erythrocytes and thrombocytes

A

innate

both responsible for movement and removal of antigens, antibodies and portions of the compliment system

Erythrocytes (RMB) and thrombocytes (platelets)

platelets - hemostasis (stopping bleeding after injury) and thrombosis (blood clots)

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7
Q

Lymphocytes (B and T cells)

A

Adaptive

B lymphocytes - mature in bone marrow, produce antibodies to neutralize pathogens

T lymphocytes - mature in bone marrow and thymus,
- helper t cells
- cytotoxic t cells

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8
Q

NK cells

A

Lymphocytes, Mature cells in the blood and spleen

Kill the cells infected with viruses, other intracellular micro-infected cells and tumor cells

  1. localized to infected tissues in response to inflammatory cytokines
  2. release cytotoxic granules, create pores in membrane, and activate a programmed death cascade
  3. release more cytokines to recruit macrophages and initiate adaptive immunity
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9
Q

How are NK cells different that the other lymphocytes (T and B cells)

A

Unlike T and b cells, NK cells do not express antigen-specific receptors, they express activating and inhibitory receptors on their surfaces that interact with lingads on the target cell

NK cells have special receptors on their surface that help them decide whether to attack a target cell.

After binding, these receptors can either send a “go ahead” signal (activating receptors) or a “stop” signal (inhibitory receptors) based on what they find on the target cell. If a target cell is acting suspiciously (like a virus-infected or cancerous cell), the NK cell will get the “go ahead” signal and attack it.

they use a more general system of activating and inhibitory receptors to determine whether to attack a cell based on its overall condition rather than specific markers. This allows NK cells to quickly respond to a wide range of threats.

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10
Q

Phagocytes

A

Phagocytes readily ingest pathogens and kill them to protect against infection

two principal families of phagocytes
- neutrophils (granulocytes, short lived 2-3 days)
- monocytes (non-granulocytes, months to years)

those undergoing radio or chemo therapy often have decreased amounts of these and re susceptible to infections

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11
Q

Antigen Presenting cells

A

Three major categories: Macrophages, dendritic cells, B cells

They display an antigen to a T cell in order to activate it, which enables the body to see and react to antigens that the body is exposed to

*Display antigens on MHC II
Class I can be recognized by cytotoxic T cells (CD8+)
Class II recognized by helper T cells (CD4+)

Macrophages and dendritic cells display the antigen after phagocytosis while B cells present the antigen through receptor-mediated endocytosis

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12
Q

First line of defense

A

innate immunity

resolve most threats, comprised of early host defense mechanisms

does not remember the interaction with a specific invader, just recognizes repeating patterns of molecular structure that are common to certain classes of pathogens

external and internal defenses

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13
Q

external innate defenses

A

physical, chemical and mechanical barriers that provide protection against invaders

skin, mucus, peristalsis, coughing, sneezing, stomach acid. cerumen, tears, saliva

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14
Q

internal innate defenses

A
  1. cellular components (neutrophils, monocytes/macrophages, NK cells)
  2. soluble factors (complement system, cytokines, chemokines, acute phase proteins)

chemokines - small cytokines that can chemotactic migration of leukocytes and enhance inflammation

acute phase response - occurs when high levels of proinflammatory cytokines are produced

cytokines - signaling molecules, don’t actually kill pathogens but recruit, organize, signal immune system factors and signal for inflammation

complement system - proteins that kill pathogens or mark them fro destruction, also promote inflammation

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15
Q

Secondary line of defense

A

Inflammatory response

includes the vascular response, the plasma protein system and the complement system

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16
Q

Vascular response of the secondary line of defense

A

vasodilation occurs to increase blood flow and decrease blood velocity

increased permeability allows migration of WBCs across vessel walls (diapedesis)

*cellular debris and fluid eventually drain through the lymphatic system, which helps mature B and T cells of the adaptive immune response

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17
Q

Third line of defense

A

Adaptive (specific) immunity

characterized by specificity and memory

humoral and cell-mediated

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18
Q

Humoral immunity

A

adaptive

involves the production of antibodies by B cells

These antibodies circulate in the blood and lymph, binding to pathogens, marking them for destruction, and neutralizing toxins

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19
Q

Cell-mediated immunity

A

adaptive

involves T cells, another type of white blood cell, which directly attack infected or abnormal cells

When T cells recognize infected cells or cancerous cells through their T cell receptors (TCRs), they can kill these cells directly (cytotoxic T cells) or help activate other immune cells (helper T cells)

20
Q

Active (adaptive) immunity

A

protection acquired by the introduction of an antigen into the host

can be natural - environmental exposure causes your body to create antibodies

or artificial - vaccine

21
Q

Passive (adaptive) immunity

A

occurs when immune products such as antibodies or sensitized lymphocytes produced by an immune person are transferred to a nonimmune person

natural - transplacental transfer of antibodies from mother to fetus, mother to infant through breast milk

artificial - admin of immune serum globulin (does not always result in the formation of memory cells)

22
Q

Variable region of antibodies

A

each antibody made by a B cell has two identical heavy chains and two identical light chains

special bonding forms it into a Y shape

the ends of the Y (where it attaches to the antigen (marker) of a pathogenic cell) are specific to each invader

the ends of the heavy chain and light chain are different from one antibody to the next and are called the variable regions. The V regions contain the HYPERVARIABLE REGIONS or CDRs which play the biggest role in making sure the antibody fits the antigen perfectly

23
Q

*constant regions of an antibody

A

Heavy chain of antibody (immunoglobin) determines the type of the antibody (IgM, IgG, IgE, IgA or IgD)

Each constant region of the immunoglobin heavy chain is encoded by a gene segment that corresponds to a lowercase Greek letter

24
Q

Major functions of immunoglobins

A
  • directly attack antigens, destroying or neutralizing them through the process of agglutination, toxins, neutralizing antigenic substances and lysing the organism’s cell wall
  • activate the complement system
  • activate anaphylaxis by releasing histamine
  • stimulate antibody-mediated hypersensitivity
25
Q

IgM

A

u

produced by and expressed on the surface of a B cell

first secreted antibody and is a predominant in a primary or initial immune response

26
Q

IgG

A

y (gamma)

major antibacterial and antiviral antibody in the blood

synthesized during a secondary immune response (after IgM)

27
Q

IgA

A

a (alpha)

in serum and secretions (saliva, breast milk, urine, tears, nasal fluids)

defends external body surfaces (mucus membrane)

28
Q

IgE

A

e (epsilon)

low levels in blood

found bound to high-affinity receptors on mast cells and basophils

fxn during allergic rxns

E for epi pen = allergic

29
Q

IgD

A

delta

low blood levels

serves as an antigen receptor on mature naive B cells

30
Q

How does the adaptive immune system produce specific responses when there are so many different pathogens that could be present

A

every characterized antibody molecule has a unique molecular signature in its variable region

B cells - As B cells develop, their DNA goes through permanent changes called gene rearrangement which allows B cells to create millions of different antigen receptors (BCRs) which is how our body can recognize many different invaders. Also, different forms of antibodies are created by RNA processing.

T cells - generation of a diverse T cell population depends upon the rearrangement of genes that comprise the variable region of the T-cell receptor

31
Q

Celled mediated immunity (Use Fig 7.16)

A

T cells

Principle fxn: destruction of microbes that are able to survive in the cytoplasm or phagocytic vesicles of infected cells

Can be harmful - responsible for the rejection of transplanted tissue and certain autoimmune diseases

For T cells to mount effector responses, they must first be stimulated through antigen recognition and then differentiate

Antigen-presenting cells (mostly dendrites) phagocytose antigens in the tissue and transport them to lymph nodes where they are presented to naive T cells via MHC molecules.

Upon activation of the T cell receptor/antigen recognition, *rapid proliferation of antigen-specific cells occurs

some naive T cells become effectors and kill the pathogen, some provide signals to other immune cells such as B cells to create antibodies, and some will become MEMORY t-cells

32
Q

T cell development

A

bone marrow to thymus gland to secondary lymphoid organs (lymph nodes and spleen)

While in the thymus, rearrangement of TCR genes occurs which determines the antigen specificity of the cells

Textbook info:

Positive selection in thymus:
- When CD4+ (helper cells) interacts with MHC class II, it receives positive selection signal to survive

  • When CD8+ cells (cytotoxic cells) interacts with MHC class I, it receives positive selection signal to survive

Negative Selection: immunologic tolerance built
- negative selection deletes T cells that recognize “self” peptides of the host (when they bind TOO strongly to MHC of self), making the immune system nonreactive to its self

failure of negative selection = autoimmune diseases

33
Q

Immunologic memory

A

cell mediated immunity

when the host is re-exposed to an antigen, a secondary immune response is generated where memory T cells rapidly differentiate into effectors that can response more rapidly with heightened immune responses

basis for vaccination!

34
Q

Helper T cells

A

CD4+ cells

Fxns;
1. help B cells make antibodies
2. activate macrophages and help them destroy pathogens
3. helping CTLs proliferate and destroy virally infected cells
4. help NK cells kill infected cells
5. neutrophil recruitment
6. downregulation of adaptive immune response

HIV infection results in gradual decline of CD4 cells

35
Q

Cytotoxic T lymphocytes (CTLs)

A

Effector cells

Primarily CD8+ and thus class I restricted (recognize antigens presented by MHC class I molecules)

controlling viral infections by directly killing virally infected cells and producing cytokines that inhibit viral replication (IFNy)

36
Q

Regulatory T lymphocyte

A

prevent inappropriate responses against self antigens of the host or commensal microorganisms

37
Q

Humoral immunity

A

adaptive

anitbodies produced by B lymphocytes present in different body fluids or secretions such as saliva blood or vaginal secretions (very effective against organisms that are free floating in the body

38
Q

B cell development (slide says not to memorize process so whats the end result)

A

Develop in bone marrow and go to secondary lymph organs (spleen and lymph)

produce antibodies

39
Q

Main Phases of the immune system

A

recognition phase - innate receptors to pathogens or antibodies bound to invaders, adaptive immune recognition occurs

amplification phase - for innate: complement cascades, production of soluble factors and recruitment of army cells (neutrophils). For adaptive; proliferation of T and B cells and differentiation into effectors, replication into clones

effector phase - removal of antigens by multiple methods

termination phase - dampens the immune system after the antigen is cleared, crucial to prevent extra damage to host

memory - generation of long lived T and B cells (lower threshold so they react faster in future)

40
Q

Factors effecting immunity

A

aging - immune system less diverse, slower response

nutrition
- reduction of normal bacteria in gut after antibiotic treatment or bc infection may interfere with nutrients availability for immune fxn in Gi tract
- calorie, protein or vitamin A ane E deficiencies = T cell fxn deficencies and decreased amount
- zinc important for T and B
- obesity

environmental pollution and chemical exposure

trauma or illness

41
Q

How does exercise impact immune system

A

depends on intensity

regular and moderate = beneficial

strenuous, prolonged = impair

42
Q

Exercise effect on nuetrophils and macrophages

A

exercise triggers rise in blood levels of neutrophils and stimulates thier acitivty along with macrophages

exercise longer than 30min = rise in nuetrophils (PMNs) over next 2-4 hours

43
Q

Exercise effect on NK cells

A

number and fxn of NK cells increase during, and immediately after exercise

temporary and probably due to rise in epinephrine levels

after intense and long exercise, concentration and activity decreases below original values

44
Q

Exercise effect on inflammatory response

A
  • brisk exercise = increased WBC count bc of increased cardiac output and epinephrine
  • lymphocytes increase but decrease below normal levels after intense exercise
  • strenuous or high intensity exercise can suppress immune fxn and damage enough tissue to provoke acute phase response
  • regular exercise will protect against low grade inflammation
45
Q

How does exercise impact aging

A

exercise regulates the immune system and delays the onset of immunosenescence (decline of fxn from aging)

  • enhanced vaccination response
  • less tire T cells and inflammation
  • increased t cell proliferation capacity and other immune cell activity
  • longer leukocyte telomere lengths
  • delays apoptosis
46
Q

Immune system and overtraining

A

can make you more susceptible to infection

neutrophils most altered with overtraining

intense exercise causes suppression of immune parameters in young people bu no huge effect on immune system of older adults (so relatively intense PA may be prescribed in elderly)

47
Q

Neck Check

A

if symptoms are above the neck exercise should be performed cautiously at half speed (if after 30 min symptoms are alleviated, continue, of they are worse, stop and rest)

if symptoms are below the neck or fever, exercise should not be initiated (aching muscles vomiting, hacking coach or diarrhea)