Immunologic / Diagnostic Techniques

Question 1

differentiate specificity fr sensitivity

Answer 1

sensitivity: % of true positive
specificity: % of true negative

Question 2

T or F. The higher the sensitivity, the higher the specificity;

Answer 2

F

inverse sila ng relationship

Question 3

Identify factor of antibody titer: how much antibody binds to the fixed amount of antigen

Answer 3

abundance

Question 4

Identify factor of antibody titer: how far the serum can be diluted before the test becomes negative

Answer 4

affinity

Question 5

__% sensitivity : precipitation test

Answer 5

90

Question 6

precipitation test for Schistosomiasis + antigen used

Answer 6

Circumoval Precipitin Test

Schistosoma japonicum egg

Question 7

If Serum (+) COPT Sample, either _ or _ is formed.

Answer 7

Large segment or Bleb

Question 8

body fluid used in COPT

Answer 8

Blood

Question 9

(+) reaction for Indirect Hemagglutination Test

Answer 9

lattice formation: cross linking of latex beads c/o antigen-antibody complexes

Question 10

in indirect fluorescent antibody test, what specimen is used as antigen spot?

Answer 10

whole parasite

Question 11

labels used in ELISA: enumerate

Answer 11

alkaline phosphatase

horseradish peroxidase

Question 12

ELISA used for neurocysticercosis

Answer 12

Magiwel Enzyme Immunoassay

Question 13

What method is used to differentiate E.histolytica fr. E.dispar?

Answer 13

Stool ELISA

Question 14

What antigen is detected in Stool ELISA for amebiasis?

Answer 14

adherence lectin

Question 15

Which is properly matched?
A.Southern Blot: RNA
B. Western Blot: protein
C. Northern Blot: DNA

Answer 15

B

Question 16

PAGE : _____ Gel Electrophoresis. enum general steps!

Answer 16

Polyacrilamide

1. Ag separation
2. transfer to paper
3. incubate with serum samples
4. detect via enzyme-labelled antibody

Question 17

in PAGE: how are crude antigens separated?

Answer 17

by molecular weight

Question 18

in PAGE: proteins are transferred on ___ paper

Answer 18

nitrocellulose paper

Question 19

To monitor those still infected after intervention, you need method with high __
A. sensitivity
B. specificity
C. both

Answer 19

A

Question 20

new commercially available method for diagnosis of Schistosoma mansonai

Answer 20

circulating cathodic antigen

Question 21

How does animal bioassay for cercariometry work?

Answer 21

inoculate organisms with water sample to test how many cercaria are there in the sample

Question 22

Which method can detect if cercaria is alive?
A. mouse bioassay
B. PCR
C. Both

Answer 22

A

Question 23

identify

exponentially progressing enzymatic synthesis of defined target DNA sequence in-vitro

Answer 23

PCR

Question 24

the only enzyme used in PCR is __

Answer 24

DNA polymerase

Question 25

substance used in PCR that provides ions needed for enzyme reaction

Answer 25

MgCl2

Question 26

basic steps of PCR: enumerate

Answer 26

denaturation annealing extension

Question 27

Which two statements are correct? A. Forward primer anneals to DNA sense strand. B. Reverse primer anneals to DNA sense strand. C. Forward primer anneals to DNA anti-sense strand. D. Reverse primer anneals to DNA anti-sense strand.

Answer 27

B and C

Question 28

Annealing temp dep on ______

Answer 28

primer sequence

Question 29

temp for denaturation @ PCR

Answer 29

95 deg C

Question 30

temp for annealing @ PCR

Answer 30

55 deg C

Question 31

temp for polymerization @ PCR

Answer 31

72 deg C

Question 32

PCR additive that stablizes Taq polymerase and overcomes PCR inhibitors

Answer 32

BSA

Question 33

PCR additive that keeps primer strands from forming secondary structures

Answer 33

DMSO

Question 34

PCR additive that increases PCR efficiency at high temp

Answer 34

glycerol

Question 35

Which is false? A. Decreasing gel thickness increases mobility of NA fragments. B. Decreasing ionic strength increases mobility of NA fragments. C. Nucleic acids are uniformly negatively charged.

Answer 35

C

Question 36

PCR method that uses more than 1 set of primers to amplify multiple targets in same rxn

Answer 36

multiplex PCR

Question 37

PCR method that uses two set of primers to amplify low conc of templates @ sample

Answer 37

nest PCR

Question 38

PCR methdo that uses fluorescent markers to track the number of copies of target sequence produced in each cycle

Answer 38

real-time PCR

Question 39

LAMP means __

Answer 39

Loop-Mediated Isothermal Amplification

Question 40

How many primers are used in LAMP?

Answer 40

6

Question 41

How many distinct regions in a target gene are recognized in LAMP?

Answer 41

8

Question 42

temperature range for LAMP

Answer 42

62 to 65 deg C

Question 43

visuals needed for LAMP reaction

Answer 43

fluorescence, turbidity

Question 44

What can be used for LAMP template DNA?

Answer 44

crude DNA prep

Question 45

T/F LAMP reagents can be stored at ambient temp for 2 weeks

Answer 45

T