Immunologic / Diagnostic Techniques Flashcards
differentiate specificity fr sensitivity
sensitivity: % of true positive
specificity: % of true negative
T or F. The higher the sensitivity, the higher the specificity;
F
inverse sila ng relationship
Identify factor of antibody titer: how much antibody binds to the fixed amount of antigen
abundance
Identify factor of antibody titer: how far the serum can be diluted before the test becomes negative
affinity
__% sensitivity : precipitation test
90
precipitation test for Schistosomiasis + antigen used
Circumoval Precipitin Test
Schistosoma japonicum egg
If Serum (+) COPT Sample, either _ or _ is formed.
Large segment or Bleb
body fluid used in COPT
Blood
(+) reaction for Indirect Hemagglutination Test
lattice formation: cross linking of latex beads c/o antigen-antibody complexes
in indirect fluorescent antibody test, what specimen is used as antigen spot?
whole parasite
labels used in ELISA: enumerate
alkaline phosphatase
horseradish peroxidase
ELISA used for neurocysticercosis
Magiwel Enzyme Immunoassay
What method is used to differentiate E.histolytica fr. E.dispar?
Stool ELISA
What antigen is detected in Stool ELISA for amebiasis?
adherence lectin
Which is properly matched?
A.Southern Blot: RNA
B. Western Blot: protein
C. Northern Blot: DNA
B
PAGE : _____ Gel Electrophoresis. enum general steps!
Polyacrilamide
- Ag separation
- transfer to paper
- incubate with serum samples
- detect via enzyme-labelled antibody
in PAGE: how are crude antigens separated?
by molecular weight
in PAGE: proteins are transferred on ___ paper
nitrocellulose paper
To monitor those still infected after intervention, you need method with high __
A. sensitivity
B. specificity
C. both
A
new commercially available method for diagnosis of Schistosoma mansonai
circulating cathodic antigen
How does animal bioassay for cercariometry work?
inoculate organisms with water sample to test how many cercaria are there in the sample
Which method can detect if cercaria is alive?
A. mouse bioassay
B. PCR
C. Both
A
identify
exponentially progressing enzymatic synthesis of defined target DNA sequence in-vitro
PCR
the only enzyme used in PCR is __
DNA polymerase
substance used in PCR that provides ions needed for enzyme reaction
MgCl2
basic steps of PCR: enumerate
denaturation
annealing
extension
Which two statements are correct?
A. Forward primer anneals to DNA sense strand.
B. Reverse primer anneals to DNA sense strand.
C. Forward primer anneals to DNA anti-sense strand.
D. Reverse primer anneals to DNA anti-sense strand.
B and C
Annealing temp dep on ______
primer sequence
temp for denaturation @ PCR
95 deg C
temp for annealing @ PCR
55 deg C
temp for polymerization @ PCR
72 deg C
PCR additive that stablizes Taq polymerase and overcomes PCR inhibitors
BSA
PCR additive that keeps primer strands from forming secondary structures
DMSO
PCR additive that increases PCR efficiency at high temp
glycerol
Which is false?
A. Decreasing gel thickness increases mobility of NA fragments.
B. Decreasing ionic strength increases mobility of NA fragments.
C. Nucleic acids are uniformly negatively charged.
C
PCR method that uses more than 1 set of primers to amplify multiple targets in same rxn
multiplex PCR
PCR method that uses two set of primers to amplify low conc of templates @ sample
nest PCR
PCR methdo that uses fluorescent markers to track the number of copies of target sequence produced in each cycle
real-time PCR
LAMP means __
Loop-Mediated Isothermal Amplification
How many primers are used in LAMP?
6
How many distinct regions in a target gene are recognized in LAMP?
8
temperature range for LAMP
62 to 65 deg C
visuals needed for LAMP reaction
fluorescence, turbidity
What can be used for LAMP template DNA?
crude DNA prep
T/F LAMP reagents can be stored at ambient temp for 2 weeks
T
