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PH 172 > Immunologic / Diagnostic Techniques > Flashcards

Immunologic / Diagnostic Techniques Flashcards

1
Q

differentiate specificity fr sensitivity

A

sensitivity: % of true positive
specificity: % of true negative

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2
Q

T or F. The higher the sensitivity, the higher the specificity;

A

F

inverse sila ng relationship

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3
Q

Identify factor of antibody titer: how much antibody binds to the fixed amount of antigen

A

abundance

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4
Q

Identify factor of antibody titer: how far the serum can be diluted before the test becomes negative

A

affinity

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5
Q

__% sensitivity : precipitation test

A

90

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6
Q

precipitation test for Schistosomiasis + antigen used

A

Circumoval Precipitin Test

Schistosoma japonicum egg

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7
Q

If Serum (+) COPT Sample, either _ or _ is formed.

A

Large segment or Bleb

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8
Q

body fluid used in COPT

A

Blood

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9
Q

(+) reaction for Indirect Hemagglutination Test

A

lattice formation: cross linking of latex beads c/o antigen-antibody complexes

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10
Q

in indirect fluorescent antibody test, what specimen is used as antigen spot?

A

whole parasite

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11
Q

labels used in ELISA: enumerate

A

alkaline phosphatase

horseradish peroxidase

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12
Q

ELISA used for neurocysticercosis

A

Magiwel Enzyme Immunoassay

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13
Q

What method is used to differentiate E.histolytica fr. E.dispar?

A

Stool ELISA

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14
Q

What antigen is detected in Stool ELISA for amebiasis?

A

adherence lectin

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15
Q

Which is properly matched?
A.Southern Blot: RNA
B. Western Blot: protein
C. Northern Blot: DNA

A

B

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16
Q

PAGE : _____ Gel Electrophoresis. enum general steps!

A

Polyacrilamide

  1. Ag separation
  2. transfer to paper
  3. incubate with serum samples
  4. detect via enzyme-labelled antibody
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17
Q

in PAGE: how are crude antigens separated?

A

by molecular weight

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18
Q

in PAGE: proteins are transferred on ___ paper

A

nitrocellulose paper

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19
Q

To monitor those still infected after intervention, you need method with high __
A. sensitivity
B. specificity
C. both

A

A

20
Q

new commercially available method for diagnosis of Schistosoma mansonai

A

circulating cathodic antigen

21
Q

How does animal bioassay for cercariometry work?

A

inoculate organisms with water sample to test how many cercaria are there in the sample

22
Q

Which method can detect if cercaria is alive?
A. mouse bioassay
B. PCR
C. Both

A

A

23
Q

identify

exponentially progressing enzymatic synthesis of defined target DNA sequence in-vitro

A

PCR

24
Q

the only enzyme used in PCR is __

A

DNA polymerase

25
Q

substance used in PCR that provides ions needed for enzyme reaction

A

MgCl2

26
Q

basic steps of PCR: enumerate

A

denaturation
annealing
extension

27
Q

Which two statements are correct?
A. Forward primer anneals to DNA sense strand.
B. Reverse primer anneals to DNA sense strand.
C. Forward primer anneals to DNA anti-sense strand.
D. Reverse primer anneals to DNA anti-sense strand.

A

B and C

28
Q

Annealing temp dep on ______

A

primer sequence

29
Q

temp for denaturation @ PCR

A

95 deg C

30
Q

temp for annealing @ PCR

A

55 deg C

31
Q

temp for polymerization @ PCR

A

72 deg C

32
Q

PCR additive that stablizes Taq polymerase and overcomes PCR inhibitors

A

BSA

33
Q

PCR additive that keeps primer strands from forming secondary structures

A

DMSO

34
Q

PCR additive that increases PCR efficiency at high temp

A

glycerol

35
Q

Which is false?
A. Decreasing gel thickness increases mobility of NA fragments.
B. Decreasing ionic strength increases mobility of NA fragments.
C. Nucleic acids are uniformly negatively charged.

A

C

36
Q

PCR method that uses more than 1 set of primers to amplify multiple targets in same rxn

A

multiplex PCR

37
Q

PCR method that uses two set of primers to amplify low conc of templates @ sample

A

nest PCR

38
Q

PCR methdo that uses fluorescent markers to track the number of copies of target sequence produced in each cycle

A

real-time PCR

39
Q

LAMP means __

A

Loop-Mediated Isothermal Amplification

40
Q

How many primers are used in LAMP?

A

6

41
Q

How many distinct regions in a target gene are recognized in LAMP?

A

8

42
Q

temperature range for LAMP

A

62 to 65 deg C

43
Q

visuals needed for LAMP reaction

A

fluorescence, turbidity

44
Q

What can be used for LAMP template DNA?

A

crude DNA prep

45
Q

T/F LAMP reagents can be stored at ambient temp for 2 weeks

A

T

PH 172 (25 decks)

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