Immunogenetics - Thiele

Question 1

T/F: Heavy chains: two for each immunoglobulin molecule and they are identical

Answer 1

True- Light chains are identical too but are either Kappa (80%) or Lambda

Question 2

Immunoglobulin gene rearrangement happens in the presence/absence of antigen?

Answer 2

Absence

Question 3

T/F: Heavy chains have VJ while light chains have VDJ

Answer 3

False

Question 4

_______ Chain rearrangement begins first in the absence of antigen in the primary lymphoid tissues

Answer 4

Heavy

Question 5

___ combines with C which most often codes for IgM constant region or IgD.

Answer 5

VDJ

Question 6

Once VJ try to combine up to 3 times on the light chain, does the cell die?

Answer 6

IgLambda light chain rearrangement occurs only after kappa light chain does not successfully rearrange.

Question 7

T/F: Once rearrangement has occurred successfully, IgM is placed on the cell surface as a receptor?

Answer 7

True- Membrane bound, monomer on the cell surface (sIg), becomes pentamer in circulation, when secreted by plasma cell

Question 8

Germ line theory of diversity: (5 parts)

Answer 8

1) Multiple genes
2) Somatic mutation (only with B cells/presence of antigen)
3) Somatic recombination
4) Gene conversion
5) Recombinatorial imprecision

Question 9

Heavy chain re-arrangement occurs following Ig gene rearrangement in the _________ lymphoid tissue and is have antigen/non-antigen dependent.

Answer 9

Secondary, antigen dependent

Question 10

T Cell variability is similar to Ig rearrangements and the mechanism is exactly like B cells except they only have 2 chains. Either alpha (light) and _______ (heavy) chains or ______ (light) and delta (heavy) chains

Answer 10

beta, gamma

Question 11

T cell gene rearrangements occur ___________ for the alpha and beta, or gamma and delta genes

Answer 11

Simutaneously

Question 12

B-cells see ___ amino acids while T-cells see a peptide at ~____ amino acids

Answer 12

125, 10

Question 13

CD3 molecule is always on what type of cell

Answer 13

T cell

Question 14

Anything that starts with “D” is class ____ and everything else (A,B,C) is class ___

Answer 14

2, 1

Question 15

CD4 binds:

Answer 15

MHC class 2

Question 16

All A, B, or C (class 1) have the same basic structure, differ at the __________ site by the peptides they can bind.

Answer 16

Binding- Class 2 looks just like class 1 but has two distinct polypeptide chains

Question 17

Function of Class 1 is to present peptide to the:

Answer 17

T cell receptor- So does Class 2

Question 18

MHC class 1 is found on all ________ cells while MHC class II is found on all _____________ cells

Answer 18

Nucleated (not RBC), antigen presenting cells (Macrophage, dendritic, B cells)

Question 19

Binding of antibodies to antigens is NOT _____ and follows Michaelis-Menton kinetics

Answer 19

STATIC

Question 20

MHC Class II is made in the ________ __________ and the _________ is cleaved into fragments

Answer 20

Endoplasmic reticulum, antigen

Question 21

MHC Class I senses inside/outside cell and presents it to the immune system

Answer 21

Inside- Made in the endoplasmic reticulum as well just like Class II but the difference is INSIDE the cell