Immobilised enzymes Flashcards
What are the advantages of isolating enzymes?
less wasteful, more specific, maximised efficiency and less downstream processing
Why are most enzymes that are isolated extracellular?
secreted from cell so easier to isolate, cells produce less extracellular enzymes so it’s easier to identify the correct one, they are more robust as conditions outside the cell are less controlled
Why would intracellular enzymes be isolated?
bigger range of them so better chance of finding one that’s ideal for the role needed.
What is the difference between isolated and immobilised enzymes?
isolated enzymes can be free where as immobilised ones are attached to an inert support system
What are the advantages of immobilised enzymes?
reusable, easily separated form product so less downstream processing, more reliable as there’s a high degree of control, greater temperature tolerance, ease of manipulation
What are the disadvantages of immobilised enzymes?
reduced efficiency, high initial cost, more technical issues
How are enzymes immobilised?
adsorption to inorganic carrier, entrapment in a matrix, covalently or ionically bonded to an inorganic carrier and encapsulated
What is an example of an enzyme being immobilised?
immobilised lactase used to produce lactose free milk as it hydrolyses lactase into glucose and galactose
