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Collège royal micro 2024 > Identification en bactériologie > Flashcards

Identification en bactériologie Flashcards

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1
Q

Donnez le résultat sur TSI pour S. typhi

A

K/A/H2S faible + (moustache)/Gaz -

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2
Q

Donnez les résultats de l’antigène O et Vi pour S.typhi.

A

O = D9 ou D12
Vi = positif

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3
Q

In what Salmonella species the heat labile capsular Vi antigen can be detected?

A

Mainly in S.typhi and S.paratyphi C

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4
Q

What are the 3 species in the S.aureus complex?

A
  • S.aureus
  • S.argenteus
  • S. schweitzeri
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5
Q

Prélèvement d’un liquide articulaire, BGP (sur la photo il semblait facilement décolorable), catalase +, indole +

A

Cutibacterium acnes

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6
Q

M35 : What is the expected reaction for N.gonorrhea for
1. γ-glutamyl-aminopeptidase
2. ß-galactosidase

A
  1. Negative
  2. Negative
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6
Q

Nommez 3 BGN entéropathogènes au genre qui sont aérobiques, oxydase (+) et B-hémol

A
  • Vibrio (fluvialis, mimicus et parfois cholerae)
  • Aeromonas
  • Grimontia (Grimontia hollisae est le seul “vibrio” qui ne pousse pas sur TCBS, il est beta-hémol et immobile)
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7
Q

M35 - which organisms can resemble Brucella on plates and are also urease positive?

A
  • Bordetella bronchiseptica
  • Oligella ureolytica
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8
Q

What temperature should be used for Campylobacter recovery?

A

42°C

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9
Q

M35 : Presence of a rosette structure (thin pleomorphic gram negative) in Gram stains made from positive blood culture broths is virtually pathognomonic for what organism?

A

Cardiobacterium hominis

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10
Q

M35 : This is the only MacConkey-negative,
catalase-negative, oxidase-positive, gram-negative rod that is ornithine positive.

A

Eikenella corrodens

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11
Q

M35 : Which of the HACEK organisms grows very well on blood sheep agar, producing large B-hemolytic colonies?

A

Kingella kingae

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12
Q

M35 : For gram-negative diplococci that are oxidase and catalase positive, and grow on both chocolate and BAP, what kind of rapid test (3) will identify M. catarrhalis?

A
  1. Positive rapid butyrate esterase
  2. Positive tributyrin test
  3. Positive indoxyl acetate test
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13
Q

M35 : What is the expected reaction for N.meningitidis for
1. γ-glutamyl-aminopeptidase
2. ß-galactosidase

A
  1. Positive
  2. Negative
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14
Q

M35 : What is the expected reaction for N.lactamica for
1. γ-glutamyl-aminopeptidase
2. ß-galactosidase

A
  1. Negative
  2. Positive (LACTamica - B-gaLACTosidase)
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15
Q

M35 : Describe indole reaction for -
1. P.mirabilis
2. P.vulgaris

A
  1. Negative
  2. Positive
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16
Q

M35 : Rare Aeromonas isolates may resemble
P. aeruginosa (lacking the typical smell), but can be distinguished by what test?

A

Indole test (Aeromonas will be positive, Pseudomonas negative)

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17
Q

M35 : Describe the expected reactions for Brucella
1. Oxidase
2. Catalase
3. MacConkey
4. Urea
5. Indole
6. Hemolysis on BAP

A
  1. Oxidase +
  2. Catalase +
  3. MacConkey No growth
  4. Urea +
  5. Indole -
  6. Hemolysis on BAP -
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18
Q

M35 : Describe the expected reactions for Campylobacter
1. Oxidase
2. Catalase
3. Motility
4. Hippurate reaction for C.jenuni
5. Indoxyl acetate reaction for C.jenuni/coli

A
  1. Oxidase +
  2. Catalase +
  3. Motility : Darting
  4. Hippurate reaction for C.jenuni : +(other species are negative)
  5. Indoxyl acetate reaction for C.jenuni/coli : +
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19
Q

M35 : Describe gram stain for Cadiobacterium hominis

A

Pleomorphic thin gram-negative rod

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20
Q

M35 : Describe the expected reactions for Cardiobacterium hominis
1. Oxidase
2. Catalase
3. Growth on MacConkey
4. Indole
5. Hemolysis on BAP
6. Nitrate

A
  1. Oxidase +
  2. Catalase -
  3. Growth on MacConkey -
  4. Indole +
  5. Hemolysis on BAP -
  6. Nitrate - (VS Pasteurella bettyae nitrate +)
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21
Q

M35 : Describe the expected reactions for Eikenella corrodens
1. Oxidase
2. Catalase
3. Indole
4. Hemolysis on BAP
5. Smell
6. ODC

A
  1. Oxidase +
  2. Catalase -
  3. Indole -
  4. Hemolysis on BAP -
  5. Smell : Bleach
  6. ODC +
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22
Q

M35 : Describe gram stain for Eikenella corrodens

A

Small gram negative rods

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23
Q

M35 : Describe the expected reactions for Escherichia coli
1. Oxidase
2. Indole
3. Hemolysis on BAP
4. PYR
5. MUG

A
  1. Oxidase -
  2. Indole +
  3. Hemolysis on BAP +
  4. PYR -
  5. MUG +
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24
Q

M35 : Describe the expected reactions for Francisella tularensis
1. Oxidase
2. Catalase
3. Growth on BAP
4. Satellites around S.aureus streak
5. B-lactamase

A
  1. Oxidase -
  2. Catalase - or weak
  3. Growth on BAP -
  4. Satellites around S.aureus streak on BAP -
  5. B-lactamase +
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25
Q

M35 : Describe the expected reactions for H.influenzae
1. Growth on BAP
2. Satellites around S.aureus streak
3. ALA test

A
  1. Growth on BAP -
  2. Satellites around S.aureus streak +
  3. ALA test -
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26
Q

M35 : Describe gram stain for Kingella kingae

A

Gram-negative coccoid rod

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27
Q

M35 : Describe the expected reactions for Kingella kingae
1. Oxidase
2. Catalase
3. Growth on MacConkey
4. Hemolysis on BAP

A
  1. Oxidase +
  2. Catalase -
  3. Growth on MacConkey -
  4. Hemolysis on BAP +
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28
Q

M35 : What test can help differentiate S.schleiferi and S.lugdunensis (both slide coagulase positive) from S.aureus?

A

S.chleiferi and S.lugdunensis are both strong PYR positive, S.aureus is PYR negative or weak reaction

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29
Q

M35 : Describe the expected reactions for S.lugdunensis
- Catalase
- Tube coagulase
- PYR
- Polymyxin B susceptibility
- Rapid ornithine
- Slide coagulase

A
  • Catalase +
  • Tube coagulase -
  • PYR +
  • Polymyxin B susceptibility : resistant
  • Rapid ornithine +
  • Slide coagulase +/clumping
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30
Q

M35 : Describe the expected reactions for Enterococcus
- Catalase
- Colony morphology on BAP
- PYR
- LAP

A
  • Catalase -
  • Colony morphology on BAP : Usually non-hemolytic, > 1mm
  • PYR +
  • LAP + ONLY required if alpha-hemolytic colony
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31
Q

M35 : Describe the expected reactions for Aerococcus viridans
- Gram stain
- Catalase
- Hemolysis on BAP
- PYR
- LAP

A
  • Gram stain : GPC in tetrads and clusters
  • Catalase -
  • Hemolysis on BAP : alpha
  • PYR +
  • LAP -
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32
Q

M35 : Describe the expected reactions for Listeria monocytogenes
- Gram stain
- Catalase
- Hemolysis on BAP
- Motility pattern
- Hippurate

A
  • Gram stain : tiny gram positive rod
  • Catalase +
  • Hemolysis on BAP : B-hemolytic, narrow zone
  • Motility pattern : tumbling at room temp, non motile at 35
  • Hippurate +
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33
Q

M35 : Describe the expected reactions for S.agalactiae
- Catalase
- Hemolysis on BAP
- Hippurate
- CAMP
- Lancefield group agglutination

A
  • Catalase -
  • Hemolysis on BAP : B-hemolytic, small zone
  • Hippurate + ONLY if B-hemol strain
  • CAMP +
  • Lancefield group agglutination : B
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34
Q

M35 : Describe the expected reactions for S.anginosus group
- Catalase
- Colony morphology
- Odor
- Lancefield group agglutination

A
  • Catalase -
  • Colony morphology : <0.5mm diameter with variable hemolysis
  • Odor : butterscotch
  • Lancefield group agglutination : always group F, sometimes A, C, G
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35
Q

M35 : Describe the expected reactions for S.pneumoniae
- Gram stain
- Catalase
- Hemolysis on BAP
- Bile solubility

A
  • Gram stain : GPC lancet shaped cocci in pairs
  • Catalase -
  • Hemolysis on BAP : alpha hemolysis
  • Bile solubility positive
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36
Q

M35 : Describe the expected reactions for S.pyogenes
- Catalase
- PYR
- Hemolysis on BAP
- Colony type
- Lancefield group agglutination

A
  • Catalase -
  • PYR +
  • Hemolysis on BAP : narrow B-hemol
  • Colony type : always >0.5mm
  • Lancefield group agglutination : A
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37
Q

M35 : Describe the expected reactions for S.viridans
- Catalase
- Hemolysis on BAP
- PYR
- LAP
- Bile solubility

A
  • Catalase -
  • Hemolysis on BAP : alpha or non hemol
  • PYR -
  • LAP +
  • Bile solubility -
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38
Q

M35 : name 5 pathogens that can look like S.viridans on agars

A
  • Pediococcus
  • Leuconostoc
  • Aerococcus
  • Granulicatella
  • Abiotrophia
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39
Q

M35 anaerobes gram negatives : Large (>1 mm diameter), convex colonies on anaerobic blood agar (eg Brucella agar) exhibiting similar-sized dark (gray or black) colonies on BBE, and seen as regular gram-negative rods on Gram stain, are identified as

A

Bacteroides fragilis group

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40
Q

M35 anaerobes gram negatives : Breadcrumb or opalescent colonies on aBAP that fail to grow on BBE; are spot indole positive; and exhibit thin, pointed, fusiform cells on Gram stain are identified as

A

F. nucleatum

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41
Q

M35 anaerobes gram negatives : Small (<1 mm diameter), translucent colonies on aBAP with corresponding tiny colonies on BBE after at least 48 hours incubation, often displaying a black dot in the center of the colony due to H2S production that are strongly catalase positive are identified as

A

Bilophila wadsworthia

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42
Q

M35 anaerobes gram negatives : On aBAPs and LKV, black-pigmented colonies (or those that fluoresce brick-red under long-wave ultraviolet light) with corresponding small, translucent or opaque colonies that consist of small
coccobacilli are identified as ___________. If they are spot indole positive, they can be identified as
_____________.

A
  1. Prevotella spp
  2. Prevotella intermedia
43
Q

M35 anaerobes gram negatives : On aBAPs, black-pigmented colonies (or those that fluoresce brick-red under long-wave ultraviolet [UV] light) with corresponding small, translucent or opaque colonies that consist of small coccobacilli, no growth on LKV and indole positive are identified as

A

Porphyromonas

44
Q

M35 anaerobes gram negatives : Flat, transparent colonies that pit the agar, are catalase negative, urease positive,
and fail to grow on BBE are

A

Bacteroides ureolyticus

45
Q

M35 anaerobes gram negatives : Small (0.5- to 1-mm diameter) transparent-to-opaque colonies on aBAP that fluoresce red under UV light, fail to grow on BBE, and exhibit tiny, gram-negative diplococci on Gram stain are

A

Veillonella spp.

46
Q

M35 anaerobes gram positive : Organisms with large (>2 mm diameter), irregular colonies exhibiting a double zone of beta hemolysis on aBAP; no growth on BBE; catalase negative; and large, boxcar-shaped, blunt-ended, gram-positive or gram-variable cells without spores on Gram stain are

A

Clostridium perfringens.

47
Q

M35 anaerobes gram positive : An anaerobic organism that shows smoothly swarming growth over the surface of the aBAP that is negative for spot indole and catalase and displays thin gram-positive rods with swollen, subterminal spores on Gram stain is

A

C. septicum

48
Q

M35 anaerobes gram positive : An anaerobic organism exhibiting slowly moving swarming
growth that is spot indole positive, catalase negative, and whose gram-positive cells exhibit swollen terminal spores (tennis racquet shaped) is

A

C. tetani

49
Q

M35 anaerobes gram positive : Another swarming Clostridium species with serpentine-edged colonies and slowly spreading growth that is spot indole and rapid urease positive, catalase negative, with subterminal spores is

A

C.sordellii

50
Q

Organisms with small (approximately 1 mm to 2 mm diameter), enamel-white, opaque colonies on aBAP that fail to grow on BBE, are spot indole positive, catalase positive, and reveal pleomorphic, coryneform rods on Gram stain are

A

Cutibacterium acnes

51
Q

Nommer 3 Streptococcus, autre que le S. pyogenes, qui sont PYR +

A

S. porcinus
S. pseudoporcinus
S. iniae

52
Q

Nommer 2 Streptococcus autre que le S. pyogenes, qui peuvent agglutiner dans le groupe A de Lancefield

A

S. anginosus group
S. dysgalactiae subsp. equisimilis

53
Q

Nommer des tests biochimiques permettant de différencier le Listeria et l’Erysipelothrix (5)

A

Catalase (Listeria + / Erysipelothrix -)
H2S (Listeria - / Erysipelothrix +)
Esculine (Listeria + / Erysipelothrix -)
CAMP (Listeria + / Erysipelothrix -)
Mobilité (Listeria + / Erysipelothrix -)

54
Q

Quels sont les géloses sélectives pour N.gonorrhoeae?

A

o Thayer-Martin : Chocolate agar + Vancomycin + Colistin + Nystatin
o Modified Thayer-Martin : Same as above + TMP to prevent swarming Proteus
o Martin-Lewis : Same as above, but has anisomycin instead of nystatin, which has increased activity against C.albicans. Also has higher concentrations of vancomycin.
o GC-Lect Agar : Additional lincomycin provides further control against Capnocytophaga species and vancomycin resistant gram positive. Is a good agar to inhibit oropharyngeal flora.
o NY city agar : Clear peptone-corn starch agar containing yeast dialysate, citrated horse plasma, lysed horse erythrocytes. Contains vancomycin, colistin, amphotericin-B and TMP.

À noter que N. meningitidis peut tout de même pousser sur ces milieux

55
Q

3 tests qui permettent de différencier P.aeruginosa des autres Pseudomonas.

A

-P.aeruginosa est pyocyanine positif
-P.aeruginosa croit at 42°C
-P.aeruginosa hydrolyse l’acétamide

56
Q

Principale façon de différencier Campylobacter de Arcobacter?

A

Arcobacter poussera seulement à 25-37°, ne poussera pas à 42° comme Campylobacter

57
Q

Quelle bactérie sera A/A au TSI mais K/A sur Kligler?

A

Yersinia enterocolitica

58
Q

Nomme 3 GPB non embranchés, aérobiques et catalase neg.

A
  • Erysipelothrix
  • Arcanobacterium
  • Trueperella
59
Q

Quels sont les tests pour différencier C. diphtheriae de C. ulcerans et C. pseudotuberculosis?

A

C.diphtheriae nitrate reduction positive (vs non pour les 2 autres)
C. ulcerans et C. pseudotuberculosis CAMP inhibition et urée positive (vs négatif pour C.diphtheriae)

60
Q

Bactéries avec un reverse CAMP (avec strie de S.agalactiae) positif (2)

A

C.perfringens
Arcanobacterium haemolyticum (peut aussi faire un CAMP inhibé)

61
Q

CBGN oxydase +, catalase +, ODC +, indol +, McConkey-

A

Pasteurella multocida (similar appearance to Brucella, but is indole positive and urease negative, Brucella is indole neg and urease pos)

62
Q

Nommez 3 géloses pour culture de Bordetella.

A

Regan-Lowe agar, Bordet-Gengou agar, and Stainer- Scholte synthetic medium

63
Q

4 espèces de Vibrio qui peuvent être BH

A
  • V.cholera
  • V.fluvialis
  • V.mimicus
  • V.damsela
64
Q

Quelles sont les espèces dans le Enterobacter cloacae complex?

A

Enterobacter cloacae, Enterobacter asburiae, Enterobacter hormaechei, Enterobacter kobei, Enterobacter ludwigii

65
Q

Quels Gram - anaérobes sont Indole + ? (3)

A

F. nucleatum
Porphyromonas spp.
Prevotella intermedia

66
Q

Quels Gram + anaérobes sont Indole + ? (3)

A

C. sordellii
C. tetani
C. acnes
Peptostreptococcus

67
Q

Quels sont les résultats attendus pour les disques de K, V et C pour les B. frag?

A

R
R
R

68
Q

Quels sont les résultats attendus pour les disques de K, V et C pour les Fusobacterium spp?

A

S
R
S

69
Q

Quels sont les résultats attendus pour les disques de K, V et C pour les Veillonella spp?

A

S
R
S

70
Q

Quels sont les résultats attendus pour les disques de K, V et C pour les Porphyromonas spp?

A

R
S
R

71
Q

Quelles bactéries ana sont R à la bile 20% ?

A

B. frag groupe
Bilophila wadsworthia
F. mortiferum
F. varium
Parabacteroides spp
Odoribacter denitrificans

72
Q

Quels Clostridium sont aérotolérants?

A

C. tertium
C. histolyticum
C. carnis

73
Q

Quels Clostridium sont immobiles?

A

C. ramosum
C. innocum
C. perfringens

74
Q

Quels clostridium ont une zone de double hémolyse?

A

C. perfringens
C. noyvi

75
Q

Quels Pseudomonas sont Oxydase - ?

A

P. oryzhabitans
P. luteola

76
Q

Quelles bactéries sont H2S + au TSI ?

A

Citrobacter spp
Proteus vulgaris/mirabilis
Edwardsiella tarda
Salmonella enterica (typhi et paratyphi)

77
Q

Quelles bactéries sont Oxydase + et poussent en présence de NaCl 6,5% ?

A

La liste est longue mais entre autre :
Vibrio vulnificus
Vibrio parahemolyticus
Vibrio algolyticus
Vibrio fluvialis

78
Q

Quelle est la seule entérobactérie oxydase + ?

A

Plesiomonas shigelloides

79
Q

À part les test phénotypiques, comment peut-on différencier un S. pneumo d’un S. viridans?

A

Le séquençage du gène rpoB ou tuf

MALDI-TOF et ARN16s incapables de les différencier

80
Q

Quels Streptococcus sont CAMP + ?

A

S. agalactiae (gène cfd)
S. iniae
S. pseudoporcinus
S. porcinus

81
Q

Quelles bactéries ont un CAMP inversé positif ? (2)

A

C. perfringens (phospholipase C)
Arcanobacterium haemolyticum

(parfois vu avec C. ulcerans et C. pseudotuberculosis aussi)

82
Q

Quels sont les deux Enterococcus mobiles?

A

E. casseliflavus
E. gallinarum

** Aussi les 2 Enterococcus ayant une R intrinsèque à la Vanco (vanC)

83
Q

Nommer 8 CGP, catalase - et anaérobie facultatif.

A
  • Abiotrophia
  • Granulicatella
  • Gemella
  • Leuconostoc
  • Pediococcus
  • Vagococcus
  • Alliococcus
  • Aerococcus
84
Q

Quelles bactéries ont une forme d’épingle au Gram?

A
  • Yersinia pestis (plus classique à la coloration Wright, Giemsa, Wayson)
  • Burkholderia pseudomallei et mallei
  • Vibrio parahemolyticus
  • Haemophilus ducreyi
  • Klebsiella granulomatis
85
Q

Quels sont les groupes de Lancefield dans lesquels les Streptococcus groupe anginosus agglutinent.

A

Groupes A, C, F et G

86
Q

Nommez 5 BGP aérobie qui peuvent être catalase négatif.

A

ELGA(R)
Erysipelothrix rhusiopathiae
Lactobacillus spp.
Gardnerella spp.
Arcanobacterium spp.
Rothia (peut être catalase + aussi)

Truepurella aussi

87
Q

BGP aérobie qui sont CAMP positif.

A
  • Listeria monocytogenes
  • Rhodococcus equi
  • Turicella otitidis
88
Q

5 tests phénotypiques qui peuvent aider à l’identification de B.anthracis :

A
  1. Mobilité (non mobile)
  2. Capsule (encre de chine)
  3. Test de lyse par bactériophage Gamma
  4. PCR visant plasmide pOX1 et pOX2
  5. Test de ténacité + pour B. anthracis
89
Q

Which species of Vibrio will appear yellow on TCBS?

A
  • V.cholera
  • V. alginolyticus
  • V. fluvialis
    (a few others but these are the important ones)
90
Q

What is the test that can differentiate Vibrio from Aeromonas?

A

String test : In this test, organisms are emulsified in 0.5% sodium deoxycholate, which lyses Vibrio cells, but not those of Aeromonas spp.

91
Q

What are the 2 species of Vibrio that do not require salt for growth?

A
  • V.cholera
  • V.mimicus
92
Q

What are the factors needed by Granulicatella and Abiotrophia for growth?

A

Cysteine or pyridoxal

93
Q

Name 2 species of Lactobacillus intrinsically resistant to vancomycin

A

L. casei
L. rhamnosus

94
Q

Tests pour identification de S.maltophila.
- Oxydase
- Mobilité
- Maltose
- Indole
- DNAse

A
  • Oxydase - (20% sont +)
  • Mobilité +
  • Maltose +
  • Indole -
  • DNAse +
95
Q

Quel espèce de Burkholderia est non motile?

A

B.mallei

96
Q

Quels sont les tests qui permettent de différencier les BGP partiellement acid fast / embranchés?

A
  • Partially acid fast or not
  • Apparence sur tap water agar (hyphen, branching)
  • Lyzozyme resistance
  • Urea hydrolysis
97
Q

Name 2 aerobic GPB that can have extensive branching.

A
  • Nocardia
  • Streptomyces

Less extensive :
- Gordonia

98
Q

Donner des tests, ou caractéristiques de croissance, qui permettent de différencier le N. gono du N. meningitidis.

A
  • Superoxol = positif pour le N. gono
  • Croissance faible/nulle du N. gono sur une GS, vs croissance normale du N. meningitidis
  • Croissance possible du N. méningitidis sur MH au sang de mouton
  • Méningo sur gélose choco: colonies grises avec décoloration verdâtre de la gélose (vs colonies plus blanches sans décoloration pour gono)
  • Gono : R au colistin
  • Méningocoque maltose +
99
Q

Nommez les 5 Staphylococcus ayant un clumping factor positif

A

S. aureus
S. lugdunensis
S. schleiferi
S. sciuri
S. intermedius

100
Q

Labo sentinel : Décrire les réactions attendues pour la motilité, l’hémolyse, et la sensibilité à la PNC pour :
- B. anthracis
- B. cereus
- B. cereus biovar anthracis

A
  • Il n’y a pas de cas décrits d’infection chez les humains avec B.cereus biovar anthracis
101
Q

Haemophilus can be confused with Brucella. What are 2 easy tests that can differentiate both?

A
  • Growth on blood agar (oui pour Brucella, non pour Haemophilus influenzae)
  • Satellite test with S.aureus streak
102
Q

Germe?

A

Yersinia pestis

103
Q

Quel supplément requiert F.tularensis pour pousser?

A

Cystéine

104
Q

Nommer un Staphylococcus qui n’est pas différenciable du S. aureus par ARN16S

A

S. aureus sous espèce anaerobius

105
Q

S.aureus, S.schleiferi and S.lugdunensis are slide coagulase positive. What rapid test can be done to identify S.aureus from the 2 others?

A

PYR. Will be negative (or weak positive) for S.aureus and strong positive for the other 2.

106
Q

Apart from S.agalactiae, what other 2 Streptococcus species can agglutinate Lancefield B group?

A

S.pseudoporcinus (can also be B-hemol, but it PYR+)
S.halichoeri (usually non B-hemol, is PYR +)

Collège royal micro 2024 (19 decks)

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