HPV

Question 1

HPV characteristics

Answer 1

Most common viral infxn of the reproductive tract
Icosahedral circular dsDNA
Viral protein L2
Forms microscopic koilocytes

• *VPL2
• escorts genomic DNA to nucleus of cell to integrate

Question 2

Symptoms of HPV

Answer 2

Warts
Cancer
Respiratory Papillomatosis

Question 3

Classifications of HPV warts

Answer 3

Genital- cauliform-like on vulva, vagina, anal, penis, scrotum (most common manifestation)
Common-rough, raised bumps on upper limb
Plantar-hard, grainyon feet
Flat- flat but raised lesions on skin

Question 4

Target cells of HPV:

Answer 4

squamous epithelium of skin and mucous membrane

Question 5

HPV entry

Answer 5

Abrasions in skin into the basal layer

Question 6

Unsuppressed oncogene for stabilization of epidermal growth factor receptor (immune evasion)

Answer 6

E5

Question 7

Binds to p53 which regulates cell layer and growth and degrades it

Answer 7

E6

Question 8

Binds and inactivates p105rb (retinoblastoma protein) which prevents excessive cellular growth

Answer 8

E7

Question 9

Lab diagnosis

Answer 9

Genital warts presence
Acetic acid test- (+) white coloration
Pap test- cervical smears fixed with alcohol and ether
Antigen and Nucleic acid detection test- obsolete
PCR- GOLD STANDARD, able to detect most HPV types

Question 10

Types of vaccine

Answer 10

Bivalent (Cervarix)- 16+ 18
Quadrivalent (Quadrasil)- 16 18 11 6
9-valent-