HIV

Question 1

HIV classification and characteristics:

Answer 1

o Genome: 2 copies of positive polarity RNA

o Capsid symmetry: Icosahedral

o Envelope: Yes; includes gp120 and gp41 (mediate interaction between viral partical and host cell)

o Tropism: CD4+ T cells (also CD4+ monocytes, macrophages)

o Reverse transcriptase + integrase + protease = 3 E that come with the virus

Question 2

capsid protein detectable by ELISA for dx:

Answer 2

p24

Question 3

3 viral enzymes:

Answer 3

Reverse transcriptase

integrase

protease

Question 4

2 envelope glycoproteins:

Answer 4

gp 120

gp 41

Question 5

viral protein that binds CD4:

Answer 5

gp 120

Question 6

viral protein binds chemokine R, initiates fusion with host plasma membrane

Answer 6

gp 41

Question 7

What does reverse transcriptase do?

Where does it do it?

Answer 7

ssRNA –> ssDNA –> dsDNA

cytoplasm

Question 8

Enzyme that gets viral dsDNA into host DNA?

Answer 8

integrase

Question 9

last viral enzyme used to package new viruses?

Answer 9

protease

Question 10

what makes the new viral RNA and mRNA?

Answer 10

host RNA pol 2

Question 11

How is HIV tested for?

Answer 11

ELISA for anti-p24 antibodies

then

confirm with Western Blot

Question 12

which two enzymes are required for formation of the provirus?

Answer 12

reverse transcriptase

integrase

Question 13

major cause of CD4 cell loss in HIV?

Answer 13

binding of viral envelope proteins expressed on infected cell surface binds to CXCR4 –> autophagy

Question 14

co-receptors for viral binding to host cell:

Answer 14

CXCR4

CCR5

Question 15

gag?

Answer 15

capsid proteins

ie. p24

Question 16

Mutation leading to resistance to HIV infection?

Answer 16

CCR5