Histology 2: Blood and Hemopoiesis Flashcards
Relative % of total blood volume that is HCT? What is considered clinically anemia? What is considered clinically polycythemia?
Normal HCT is approx. 45% of total blood volume with plasma making up the remaining ~55%.
Anemia is an HCT below 30%
Polycythemia is HCT above 60%
Importance of Albumin?
Accounts for 1/2 of plasma protein and is made in the liver.
Most important for regulation of colloid osmotic pressure of blood, helps maintain blood/tissue fluid volumes.
Acts as carrier protein and aids in transport of hormones, metabolites and drugs.
Two important clotting factors, and function in clotting cascade.
Prothrombin: inactive form of thrombin. Thrombin in an enzyme that facilities platelet aggrigation and in the presence of Ca++ converts fibrinogen to fibrin.
Fibrinogen: largest plasma protein, converted to fibrin. Fibrin polymerizes and forms a dense network at sides of blood vessel damage (i.e. clot).
Erythrocytes: function, characteristics, 2 important plasma membrane components.
Carries gases in blood vessels.
Biconcave disk, 7.5 um diameter, 2.6 um thick at edge and 0.75 um thick at center.
Mature RBC’s do not have organelles.
Spectrin: forms network on cytoplasmic side of plasma membrane. Bind find to actin and to Ankyrin.
Ankyrin: provides binding between spectrin filaments and integral membrane proteins (band 3 protein)
Clinically important info about erythrocytes, mutations of membrane proteins, pathological morphological changes in erythrocytes.
Hereditary Spherocytosis: result of mutation in spectrin gene in that the erythrocyte is unable to maintain biconcave shape and becomes spherical.
Anisocytosis: presence of red blood cells of varying size in peripheral blood.
- -Megalocytes: abnormally large RBCs >9um
- -Poikilocytosis: irregularly shaped RBCs, w/ diameter < 6um called microcytes.
Which live longer, mononuclear agranulocytes or polymorphonuclear granulocytes?
Mononuclear agranulocytes
What types of leukocytes are polymorphonuclear granulocytes?
Neutrophils
Eosinophils
Basophils
What types of leukocytes are mononuclear agranulocytes?
Lymphocytes
Monocytes
Neutrophils: relative abundance, cellular morphology, life cycle, functions.
Comprise 60-70% of blood leukocytes, 3,000-6,000/uL blood.
Fairly large cells, multilobed nuclei, few mitochondria, often called polymorphonuclear leukocytes (PMNs).
Females have a second x-chromosome which shows up as a Barr-body on the nucleus and is drumstick shaped.
3 types of granules present:
- Azurophilic: non-specific/primary, represent lysosomes
- Neutrophilic: specific/secondary, lysozymes and lactoferrins
- Tertiary: gelatinase and collagenases
Produced in bone marrow and released into circulation. Life span of 1-4 days in tissue.
First line of defense, endocytosis of particles and then destroyed with Respiratory Burst.
Produce IL-1, which induced fever
Eosinophils: relative abundance, cellular morphology, life cycle, functions.
Rare in peripheral blood (2-4%)
Similar size to neutrophils, bilobed nucleus.
Contain eosinophilic (specific) granules and azurophilic granules.
- Cytotoxins: designed to destroy protozoan and helminthic parasites. Major Basic Protein (MBP), eosinophil cationic protein (ECP) and eosinophil peroxidase.
- Neurotoxins: eosinophil-derived neurotoxin, causes neuro dysfunction in parasites.
- Histaminase: breaks down histamine
Most common during parasitic infection (ex. trichinosis).
Phagocytize antigen-antibody complexes and modulate inflammatory response.
Also increase numbers during allergic reactions and help break down histamine and inhibit mast cell degranulation.
Basophils: relative abundance, cellular morphology, life cycle, functions.
Rares of WBCs in normal blood smears (0.5-1.5%)
Smallest granulocyte (8-10um), bilobed nucleus, contain basophilic granules which contain histamine and heparin.
Major function is the production of histamine, very similar to Mast Cells. Commonly seen during chronic granulocytic leukemia and chicken pox.
Lymphocytes: relative abundance, cellular morphology, life cycle, functions.
Second most common WBC (20-30%), most common agranulocyte.
Vary in size from 6-14um, depending on stage of development.
3 subtypes:
T-lymphocytes
B-lymphocytes
Natural Killer (KN) cells
T-lymphocytes: types, characteristics
Arise from bone marrow but mature in thymus. Characterized by T-cell receptor on surface. Produce cytokines to help recruit and direct other immune cells.
T-Helper cells:
-acive and stimulate proliferation of B-cells, stimulate proliferation of B-cells into plasma cells. Activate and proliferate T-cells, activate Mast Cells, activate Macrophages and increase phagocytosis.
T-Cytotoxic Cells (CD4):
-activated by TH cells and destroy antigen presenting cells. Responsible for destroying infected cells and also responsible for the transplant rejection.
Supressor T lymphocytes (CD8):
-suppress antibody formation by B cells, down regulate ability of T cells to initiate a cellular response.
B lymphocytes: characteristics
Arise and mature in bone marrow.
Function is to produce antibodies (immunoglobin). Reside in lymphoid and connective tissues.
Natural Killer (NK) Cells
large lymphocytes that are programmed during development to kill certain virus-containing cells and some types of tumor cells.