2017 Microbiology 1 - Prokaryotic Genetics & Medical Virology Flashcards
What is Reassortment and what types of organisms can participate?
- exchange of genomic nucleic acid segments between strains.
- can take place in some viruses and all eukaryotes
- mechanism by which “genetic shift” takes place
What is Transformation?
Uptake of extracellular DNA by bacteria in a particular physiological state (Competency)
Gram Positive bacteria that can be transformed by exogenous DNA
Streptococcus pneumoniae
S. aureus
Bacillus subtilis
Gram Negative bacteria that can be transformed by exogenous DNA
Neisseria menigitis
Neisseria gonorrhea
Haemophilus influenzae
E. coli
Process of transformation of exogenous DNA into bacteria.
- Transformasome, membrane bound organelles, sequester DNA and transfer it into the cell’s interior
- Degradation of one DNA strand takes place and the other is used in recombination
- DNA recombination may depend on specific donor DNA sequences
What is Conjugation and how does it work?
Fertility plasmid-facilitated transfer of a plasmid or host chromosome to a recipient cell.
Only occurs between strain of the same or closely related species.
Process of Conjugation in Gram-negatives?
- Extrusion of sex pilus from one bacteria
- Pilus adheres to outer membrane of Gram-negative cell walls
- Cells become bound together
- Plasmid undergoes “transfer replication”
- One strand of parental DNA is broken and transferred to the second bacteria
What is an Episome?
A plasmid capable of replicating into the chromosome.
What is the Sex Factors (F factors) and Hfr and their importance?
Sex Factors (Fertility Factors / F) are designated F+ in those that contain it. Those which lack F factors are F- When the F-plasmid is integrated into the chromosome it becomes a Hfr (High Frequency Recombination) donor cell.
F+ transfer plasmid only
Hfr transfer plasmid and some chromosomal genes
Cell properties that can be carried by plasmids:
Drug resistance Toxins Adhesins Growth Factors Production of Antimicrobial agents
What is transduction?
Can occur in Gram+ or Gram - cells when a fragment of DNA is carried to the recipient cell in a virus (Bacteriophage) produced by donor cell.
What are signs of a Viral Infection?
- dsRNA
- Expression of viral protein on surface of plasma membrane, causing activation of Tc cells, NK cells and induction of antibody synthesis
- Formation of inclusion bodies either within cytoplasm or nucleus (very rarely in both)
Cytology of Viral Infections: CPE
Viral inducted cytopathologic effects (CPE)
- inclusion bodies
- cell lysis
- vacuolation
- syncytia (multinucleated cells formed by cell fusion)
Viral Detection via CPE
- inclusions, plaquesor other cytopathological effects may be characteristic of certain viral infections.
- Heterologous interference - the presence of the infection by one virus can prevent the infection of another.
- Hemagglutination Inhibition - certain infections cause the expression of hemagglutinins (receptors) which cause RBCs to bind (hemagglutination).
Viral Detection via Quantization: TCD50, LD50, ID50
Tissue Culture Dose (TCD50) - titer of virus causing CPE in 50% of cultured cells
Lethal Dose (LD50) - titer of virus killing 50% of test animals
Infectious Dose (ID50) - titer of virus infecting 50% of test animals
Plaque forming units - concentration of particules capable of producing a hole in a bacterial lawn or tissue culture monolayer. CPE can also be the end-point in this calculation.