2017 Physiology - Smooth Muscle Flashcards

1
Q

What are 2 proteins that make up the intermediate filaments of smooth muscle?

A

Desmin and Vimentin

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2
Q

What protein are in dense bodies and what do they correspond to in striated (skeletal) muscle?

A

Actinin

Functionally analogous to Z-lines, serve as anchor for thin-filaments (Actin)

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3
Q

Does smooth muscle have terminal cisterns or T-tubules?

A

No

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4
Q

How many light and heavy subunits does smooth muscle myosin have?

A

6 total

  • 2 heavy
  • 4 light
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5
Q

Describe the innervation and communication between Single Unit smooth muscle cells?

A

Many gap junctions
Sparse innervation - no true neuromuscular junctions, instead nerves just “drop” NT on smooth muscles

Muscle behaves in a syncytial manner

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6
Q

Describe the innervation and communication between Multi Unit smooth muscle cells?

A

Tend to be more discrete activities

Fewer gap junctions
Higher Innervation ratios
-graded potentials but no real action potentials

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7
Q

What modifications take place around pregnancy in smooth muscle, specifically progesterone and estrogen.

A

Progesterone - reduce gap junctions in myometrial smooth muscle, behaves more like non-innervated multiunit smooth muscle

Estrogen - at term, smooth muscle hypertrophy, increase gap junctions - behaves more like single unit

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8
Q

What is the role of the Ca++/Calmodulin complex?

A

Binds to Myosin Light Chain Kinase (MLCK) and activates it forming an enzyme complex.

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9
Q

What is the function of the MLCK/Ca++ Calmodulin complex? What are the energy requirements?

A

Phyosphorylates the myosin light chains, requiring 1 ATP

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10
Q

What is the function of Myosin Light Chain Phosphatase (Phosphatase C)?

A

removes phosphates put on myosin light chains by MLCK/Ca++ Calmodulin complex

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11
Q

What is the energy requirement for cross-bridge cycling in smooth muscle?

A

1 ATP

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12
Q

What is the purpose of the latch state and how does it work with smooth muscle?

A

Latch state is similar to rigor in skeletal muscle, it allows maintained contraction without ATP expenditure.
The myosin light chains are de-phosphorylated and ATPase activity thus decreases.

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13
Q

What factors contribute to smooth muscle relaxation?

A

low levels of phosphorylated myosin light chains

lowering of intracellular [Ca++]

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14
Q

How is Ca++ removed from smooth muscle cells?

A

Sequestered by sarcolemmal 3Na/Ca exchanger and Sarcolemmal Ca++ ATPase.

Phosphorylation of Phospholamban (which normally inhibits SR Ca++ ATPase) by PKA/PKG

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15
Q

How many types of Ca++ channels and describe them?

A

L-type (slow) - long acting, open slowly and close slowly. Affected by Ca-channel blockers such as verapamil, nifedipine, diltiazem

T-type (transitent) - fast acting, may be key to Ca++ induced Ca++ release from SR which is sensitive to relative change in concentration not absolute concentration. NOT blocked by typical Ca++ channel blockers.

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16
Q

How does cAMP dependent relaxation work?

A

Adenosin, PGI2

PKA phosphorylates MLCK and prevents activation by Ca/Calmodulin complex.
cAMP can decease [Ca] in some cells, this occurs because cAMP causes PKA-dependent phosphorylation of phospholamban which increases Ca uptake at SR.

17
Q

How does cGMP-dependent relaxation work?

A

NO, ANP

Decrease in myosin light chain phosphorylation.

18
Q

How does Phospholipase C dependent contraction work?

A

Angiotensin II

IP3 is formed and this releases intracellular Ca++, also DAG is formed and this activates PKC.
PKC phosphorylates proteins contributing to smooth muscle contraction, including L-type Ca++ channels (lowering their threshold) and myosin light chains.