Hematology Lab Part 2

Question 1

The blood contains 3 specialized classes of cells or “formed elements”

Answer 1

Red blood cells (erythrocytes)
White blood cells (leukocytes)
Platelets (thrombocytes)

Question 2

Another name for RBC

Answer 2

Erythrocytes

Question 3

Another name for WBC

Answer 3

Leukocytes

Question 4

Another name for Platelets

Answer 4

Thrombocytes

Question 5

The function of these cells are to transport oxygen and carbon dioxide

Answer 5

RBC

Question 6

The function of these cells are to combat infections and invading organisms

Answer 6

WBC

Question 7

The function of these cells are to prevent blood loss

Answer 7

Platelets

Question 8

Normal Blood Cell Values of RBC in Males

Answer 8

5.4 +/- 0.8 million per cubic millimeter (mm^3)

Question 9

Normal Blood Cell Values of RBC in Females

Answer 9

4.8 +/- 0.6 million per cubic millimeter (mm^3)

Question 10

Normal Blood Cell Values of WBC in Males

Answer 10

7-9000 per mm^3

Question 11

Normal Blood Cell Values of WBC in Females

Answer 11

5-7000 per mm^3

Question 12

Normal Blood Cell Values of Platelets

Answer 12

150,000 to 400,000 per mm^3

average = 300,000 per mm^3

Question 13

Which are not true “cells”?

Answer 13

Erythrocytes and the Platelets;
They both lack nuclei and are unable to undergo mitosis to form daughter cells;
nothing more than “bags” to carry specific chemicals;
hemoglobin in RBC & platelet factor 3 in the platelets

Question 14

If each gram of hemoglobin in the RBC is maximally saturated with oxygen, it can carry about

Answer 14

1.34 mL of oxygen

Question 15

In each 100 mL of blood there is roughly

Answer 15

15 g of Hb, hence around 20 mL of oxygen can be carried in every 100 mL of blood

Question 16

Anemia often results from

Answer 16

an abnormal decrease in the number of erythrocytes, so that insufficient oxygen is carried to the tissues and they become oxygen starved
other factors may also cause anemia, such as decreased Hb in each cell, decreased cell size, hemorrhage, etc.

Question 17

In order to accurately diagnose the cause of anemia the total erythrocyte picture must be examined

Answer 17

hematocrit, hemoglobin, RBC count, cell size, hemoglobin in each cell, etc

Question 18

Automatic device that makes cell counts

Answer 18

Coulter Counter

Question 19

Hemocytometer

Answer 19

Each of the 2 counting chambers for counting blood cells are
9 mm squares divided into 9 squares, each measuring 1 mm square;
4 corner squares are used for counting leukocytes and are divided into 16 smaller squares
The center 1 mm^2 square is divided into 25 small squares (1/25 mm^2), and each is further subdivided into 16 smaller squares

Question 20

Leukopenia

Answer 20

Deficient number of leukocytes

Question 21

Leukocytosis

Answer 21

Abnormally high number of leukocytes

Question 22

Which chemical do RBC carry?

Answer 22

Hemoglobin

Question 23

Which chemical do platelets carry?

Answer 23

platelet factor 3

Question 24

Tool we used in our blood lab for diluting

Answer 24

Thoma Pipettes

Question 25

Purpose of Hayem’s or Gower’s solution

Answer 25

preserve the corpuscles and prevent coagulation

Question 26

The dilution of RBC was by a factor of

Answer 26

200X

Question 27

The depth of the counting chamber for RBC was by a factor of

Answer 27

10X

Question 28

The area of the RBC was multiplied by a factor of

Answer 28

25X

Question 29

The total multiplication factor for RBC is

Answer 29

200 X 10 X 25 = 50,000

Question 30

If you were to count an average of 120 RBC per 1/25 mm^2 square, your RBC count would be

Answer 30

120 X 50,000 = 6,000,000 RBC per mm^3

Question 31

By calculating the RBC size and the percentage of hemoglobin each cell carries, we are able to more accurately pinpoint the cause of

Answer 31

Anemia

Question 32

Mean Corpuscular Volume (MCV)

Answer 32

MCV = hematocrit (% red cells) X 10 / RBC count (millions/mm^3)

Question 33

Normal Range of MCV

Answer 33

87 +/- 2 cubic microns

Question 34

Mean Corpuscular Hemoglobin Concentration (MCHC)

Answer 34

MCHC (%) = hemoglobin (g/100ml blood) X 100 / hematocrit (%)

Question 35

Normal Range of MCHC

Answer 35

34 +/- 2% of the RBC is hemoglobin

Question 36

The WBC were diluted by a factor of

Answer 36

X20

Question 37

The WBC depth was by a factor of

Answer 37

X10

Question 38

The average number of cells in 1 mm^2 were counted by a factor of

Answer 38

X1

Question 39

The total multiplication factor for WBC

Answer 39

20 X 10 X 1 = 200