Gut crypt cells

Question 1

What are the cells associated with a mammalian gut crypt?

Answer 1

Mesenchymal cells
Basement membrane
Paneth cells
Stem cells (Crypt base columnar cells)
Transit amplifying cells (TA)

Question 2

What do CBCS selectively express?

Answer 2

Wnt target genes

Question 3

What are Wnt target genes?

Answer 3

Lgr5/ GPR49

Question 4

Which signals are needed to maintain epithelial stemness?

Answer 4

Wnt, Notch, EGF)

Question 5

What signal negatively regulates stemness?

Answer 5

BMP

Question 6

What are the 3 applications of creating a mini gut?

Answer 6

1. Experimental tool
2. Diagnostic tool
3. Therapeutic tool

Question 7

What are the steps in making a mini gut?

Answer 7

1. Lrg5+ cells are genetically labelled by EGFP- sorted and embedded in matrigel
2. A single SC forms a cyst- bud formation occurs- symmetry is broke, structure resembles a crypt
3. Organoids adhere to de-epitheliazed wound bed, forms a flat epithelial layer, followed by crypt reconstruction
4. Wnt5+ mesenchymal cells support crypt formation in the damaged area