Genomics

Question 1

Craig venter

Answer 1

-major name in DNA sequencing
-shotgun sequencing to sequence random bits of DNA and let computers figure out how it all fits together

Question 2

Sanger sequencing

Answer 2

-Based on DNA polymerase building complementary strands using mostly normal dNTPs and rare special dNTP that lack a 3OH and therefore cannot be elongated further
-ddNTP’s labelled using different lfuorphores
-Build DNA of different lengths
-Determine sequence based on identity of terminating residues

Question 3

illumina sequencing

Answer 3

-chips generate mullions of clusters each representing many copies of a DNA molecule being sequenced

Question 4

in illumina - reversibly terminated dNTPs used to

Answer 4

-insert one lable residue, and take an image
-unblock the 3’ end so you can add another residue adn repeat
-each round you get an image of what reisude at each cluser for that position

Question 5

what can genome sequencing tell us

Answer 5

-what genes are absent and present and the sequence of each gene
-provides DNA blue print required for many studies

Question 6

what gene are present/absent in the genome of genome sequencing further explanation

Answer 6

-metabolic capabilities of an organism
-virulence genes, antibitoic resistance genes
-unusual mutations that account for unusual phenotypes
-discover new genes that might be of medical interst

Question 7

providing DNA blue print required for many studes and analysis further explanation in genome sequencing

Answer 7

-genetic appraoches
-transcriptomics -
-proteomics
-INseq

Question 8

vampirovibrio chlorellavorus

Answer 8

-eats other bacterua
-functional and metabolic predictions are from genomics

Question 9

metagenomics

Answer 9

-study of the complete genetic content of an environmental saple
-can provide info for organisms that cannot be cultures
-can tell us composition of microbial communities and how composition changes
-can also look at gene leave

Question 10

transcriptomics

Answer 10

-RNA converted to DNA using reverse trasnciption which can be sequences using next generation methods
-can get a complete picture of the relative abundance of all the trasncripts in the cell under a given set of ocnditions
-one way this is used is comparing expression under different conditions

Question 11

proteomics

Answer 11

-often relies on knowing the genomic DNA sequence but doesnt use DNA sequencing
-instead mass spectometry to identify protein and protein levels
-can tell you which proteins are present under which conditions
-can tell you whic protein itneract with a prtoein of interest