Genomic Sequencing - Genetechnology Flashcards
1
Q
1.
A
Primer binds to DNA allowing DNA polymerase to attach.
2
Q
2.
A
DNA polymerase adds free nucleotides so strand increases in length.
3
Q
3.
A
When nucleotide with terminator base is added, DNA polymerase is thrown off and nucleotides can no longer be added.
4
Q
4.
A
Many copies of different lengths of DNA are made, each with a different coloured tag at its end.
5
Q
5.
A
DNA lengths are separated by electrophoresis (shorter lengths move faster). A laser at the end allows the tag colour to be recorded automatically.
6
Q
6.
A
The laser reads the colour sequence of the tagged nucleotides from the DNA until the whole base sequence is determined.
