Electrophoresis - Genetechnology Flashcards
1
Q
1.
A
DNA sample is cut into fragments by restriction endonuclease enzymes.
2
Q
2.
A
The fragments are put into wells at one end (negative) of a gel plate.
3
Q
3.
A
Current is applied for fixed amount of time.
4
Q
4.
A
DNA fragments are negative (PO4-) so diffuse towards positive end.
5
Q
5.
A
Smaller fragments move faster and further than the longer fragments.
6
Q
6.
A
The position of the fragments is shown by a dye that stains DNA.
