GALING KAY JAS Flashcards
Tissue sources of Acid phosphatase is
1. Prostate
2. Liver
3. Erythrocyte
4. Platelet
5. Kidney
→ 1,3,4
LD isoenzymes maybe useful in determining the source of injury in combination of isoenzymes H & M in following order.
→ 1 > 2 > 3 > 4 > 5
In Cherry Crandall Method for Lipase analysis what is the substrate used?
→ Triolein
The optimum pH for alkaline phosphatase is
→ 9-10
The following is/are TRUE about Endpoint reaction
1. Measurement is made of the amount the reaction has occurred.
2. It is based on the final absorbance reading.
→ Both 1 & 2
The following is/are TRUE about Kinetic reaction
1. Measurement is made of the amount the reaction has occurred.
2. It is based on the specific time interval reading.
→ 2 only
These enzymes catalyzes the removal of groups from substrates without hydrolysis. The product contains double bonds.
→ Lyases
Hydrolysis of ether and ester
1. Lipase
2. ACE
3. SGOT
→ 1 & 2
Enzyme is proportional to rate.
→ Zero-order kinetics
In other methods for ACP, storage at room temperature results in loss of enzyme activity.
→ True
________ is the only enzyme in which decrease is clinical significance.
→ Pseudocholinesterase
Which is/are TRUE about the Karmen Method for SGOT determination
1. Aspartate + α-ketoglutarate → oxaloacetate + glutamate
2. LDH is used as coupling enzyme
→ 1 only
In other methods for ALP, low results are due to improper anticoagulant, while high results is due to hyperthyroidism
→ True
Cherry Krandal Method uses triolein as a substrate. Fatty acid is measured by increased turbidity.
→ First statement is correct. Second statement is incorrect.
Major sources of Gamma-glutamyl transferase includes the following:
1. Heart
2. Liver
3. Kidney
4. Pancreas
→ 2, 3, 4
This is also known to its older terminology as Serum Glutamic oxaloacetic transaminase.
→ AST
__________ : liberation of carbon dioxide from acidic acid; _________ : decrease in pH from acetic acid
→ Manometric ; Electrometric
Kinetic Method has multiple measurement of absorbance change made at specific time interval.
→ True
ALT determination involved LDH as coupling enzyme. Diagnostic significance is for the a specific evaluation of RBC disorders and skeletal involvement. → First statement is correct,
→ First statement is correct, second statement is incorrect
Lactate Dehydrogenase has the highest activity in the heart, liver, skeletal muscle, kidney, and erythrocytes.
→ True
Plate Assays for lipase determination uses tweens or tributyrin that change the color of phenol red after complexation with fluorescent dye Rhodamine B.
→ True
Ptyalin : ___________ ; Amylopsin : __________
→ Salivary ; Pancreatic
Amylase is falsely elevated upon administration opiates as painkiller. Pancreactic amylopsin secreted from the acinar cells of the pancreas.
→ Both statements are correct.
Gamma-glutamyl transferase is the smallest enzyme.
→ False
Amylase breaks down Starch and Glycogen.
→ True
Lactate Dehydrogenase is widely distributed in tissue.
→ True
This substance is poisonous when inhaled, swallowed or when absorbed through skin produced during the reaction under reagent preparation and stability.
→ p-nitrophenol
Pyridoxal Phosphate (Vitamin B12) is the coenzyme for the AST reaction.
→ False
Is the energy required to raise all molecules to the transition state in a chemical reaction so that products may be formed?
→ Activation energy
Hemolysis should be avoided because hemoglobin inhibits amylase activity.
→ False
CK is stable due to oxidation of sulfhydryl groups.
→ False
Which is/are TRUE about Pseudocholinesterase determination:
1. Manometric method liberation of carbon dioxide from acetic acid
2. Cherry Krandal Method is the reference method
3. Decreased level is clinically significant
→ 1 & 3
PChe : ___________ ; Ache : _______________
→ White matter of nervous system ; Brain
Which is/are TRUE about Reitman and Frankel Method?
1. Color reaction is measured at 505nm due to diazonium salt coupled with keto acid.
2. 35 U/L – Within reference range
→ 1 only
__________ : bind to active site ; ______________ : bind to ES complex
→ Competitive ; Uncompetitive
Which among the following is a method of analysis for Lipase.
1. Amyloclastic
2. Chromogeninc
3. Saccharogenic
4. Titrimetric
→ None among the options
__________ : removal of sialic acid ; ___________ : bind other isoenzymes
→ Neuramidase ; Wheat Germ Lectin
Amylopsin is secreted from the Acinar Cells of the pancreas
→ True
