GALING KAY JAS Flashcards

1
Q

Tissue sources of Acid phosphatase is
1. Prostate
2. Liver
3. Erythrocyte
4. Platelet
5. Kidney

A

→ 1,3,4

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2
Q

LD isoenzymes maybe useful in determining the source of injury in combination of isoenzymes H & M in following order.

A

→ 1 > 2 > 3 > 4 > 5

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3
Q

In Cherry Crandall Method for Lipase analysis what is the substrate used?

A

→ Triolein

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4
Q

The optimum pH for alkaline phosphatase is

A

→ 9-10

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5
Q

The following is/are TRUE about Endpoint reaction
1. Measurement is made of the amount the reaction has occurred.
2. It is based on the final absorbance reading.

A

→ Both 1 & 2

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6
Q

The following is/are TRUE about Kinetic reaction
1. Measurement is made of the amount the reaction has occurred.
2. It is based on the specific time interval reading.

A

→ 2 only

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7
Q

These enzymes catalyzes the removal of groups from substrates without hydrolysis. The product contains double bonds.

A

→ Lyases

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7
Q

Hydrolysis of ether and ester
1. Lipase
2. ACE
3. SGOT

A

→ 1 & 2

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8
Q

Enzyme is proportional to rate.

A

→ Zero-order kinetics

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9
Q

In other methods for ACP, storage at room temperature results in loss of enzyme activity.

A

→ True

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10
Q

________ is the only enzyme in which decrease is clinical significance.

A

→ Pseudocholinesterase

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11
Q

Which is/are TRUE about the Karmen Method for SGOT determination
1. Aspartate + α-ketoglutarate → oxaloacetate + glutamate
2. LDH is used as coupling enzyme

A

→ 1 only

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12
Q

In other methods for ALP, low results are due to improper anticoagulant, while high results is due to hyperthyroidism

A

→ True

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13
Q

Cherry Krandal Method uses triolein as a substrate. Fatty acid is measured by increased turbidity.

A

→ First statement is correct. Second statement is incorrect.

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14
Q

Major sources of Gamma-glutamyl transferase includes the following:
1. Heart
2. Liver
3. Kidney
4. Pancreas

A

→ 2, 3, 4

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15
Q

This is also known to its older terminology as Serum Glutamic oxaloacetic transaminase.

16
Q

__________ : liberation of carbon dioxide from acidic acid; _________ : decrease in pH from acetic acid

A

→ Manometric ; Electrometric

17
Q

Kinetic Method has multiple measurement of absorbance change made at specific time interval.

18
Q

ALT determination involved LDH as coupling enzyme. Diagnostic significance is for the a specific evaluation of RBC disorders and skeletal involvement. → First statement is correct,

A

→ First statement is correct, second statement is incorrect

19
Q

Lactate Dehydrogenase has the highest activity in the heart, liver, skeletal muscle, kidney, and erythrocytes.

20
Q

Plate Assays for lipase determination uses tweens or tributyrin that change the color of phenol red after complexation with fluorescent dye Rhodamine B.

21
Q

Ptyalin : ___________ ; Amylopsin : __________

A

→ Salivary ; Pancreatic

22
Q

Amylase is falsely elevated upon administration opiates as painkiller. Pancreactic amylopsin secreted from the acinar cells of the pancreas.

A

→ Both statements are correct.

23
Q

Gamma-glutamyl transferase is the smallest enzyme.

24
Amylase breaks down Starch and Glycogen.
→ True
25
Lactate Dehydrogenase is widely distributed in tissue.
→ True
26
This substance is poisonous when inhaled, swallowed or when absorbed through skin produced during the reaction under reagent preparation and stability.
→ p-nitrophenol
27
Pyridoxal Phosphate (Vitamin B12) is the coenzyme for the AST reaction.
→ False
28
Is the energy required to raise all molecules to the transition state in a chemical reaction so that products may be formed?
→ Activation energy
29
Hemolysis should be avoided because hemoglobin inhibits amylase activity.
→ False
30
CK is stable due to oxidation of sulfhydryl groups.
→ False
31
Which is/are TRUE about Pseudocholinesterase determination: 1. Manometric method liberation of carbon dioxide from acetic acid 2. Cherry Krandal Method is the reference method 3. Decreased level is clinically significant
→ 1 & 3
32
PChe : ___________ ; Ache : _______________
→ White matter of nervous system ; Brain
33
Which is/are TRUE about Reitman and Frankel Method? 1. Color reaction is measured at 505nm due to diazonium salt coupled with keto acid. 2. 35 U/L – Within reference range
→ 1 only
34
__________ : bind to active site ; ______________ : bind to ES complex
→ Competitive ; Uncompetitive
35
Which among the following is a method of analysis for Lipase. 1. Amyloclastic 2. Chromogeninc 3. Saccharogenic 4. Titrimetric
→ None among the options
36
__________ : removal of sialic acid ; ___________ : bind other isoenzymes
→ Neuramidase ; Wheat Germ Lectin
37
Amylopsin is secreted from the Acinar Cells of the pancreas
→ True