GALING KAY JAS

Question 1

Tissue sources of Acid phosphatase is
1. Prostate
2. Liver
3. Erythrocyte
4. Platelet
5. Kidney

Answer 1

→ 1,3,4

Question 2

LD isoenzymes maybe useful in determining the source of injury in combination of isoenzymes H & M in following order.

Answer 2

→ 1 > 2 > 3 > 4 > 5

Question 3

In Cherry Crandall Method for Lipase analysis what is the substrate used?

Answer 3

→ Triolein

Question 4

The optimum pH for alkaline phosphatase is

Answer 4

→ 9-10

Question 5

The following is/are TRUE about Endpoint reaction
1. Measurement is made of the amount the reaction has occurred.
2. It is based on the final absorbance reading.

Answer 5

→ Both 1 & 2

Question 6

The following is/are TRUE about Kinetic reaction
1. Measurement is made of the amount the reaction has occurred.
2. It is based on the specific time interval reading.

Answer 6

→ 2 only

Question 7

These enzymes catalyzes the removal of groups from substrates without hydrolysis. The product contains double bonds.

Answer 7

→ Lyases

Question 7

Hydrolysis of ether and ester
1. Lipase
2. ACE
3. SGOT

Answer 7

→ 1 & 2

Question 8

Enzyme is proportional to rate.

Answer 8

→ Zero-order kinetics

Question 9

In other methods for ACP, storage at room temperature results in loss of enzyme activity.

Answer 9

→ True

Question 10

________ is the only enzyme in which decrease is clinical significance.

Answer 10

→ Pseudocholinesterase

Question 11

Which is/are TRUE about the Karmen Method for SGOT determination
1. Aspartate + α-ketoglutarate → oxaloacetate + glutamate
2. LDH is used as coupling enzyme

Answer 11

→ 1 only

Question 12

In other methods for ALP, low results are due to improper anticoagulant, while high results is due to hyperthyroidism

Answer 12

→ True

Question 13

Cherry Krandal Method uses triolein as a substrate. Fatty acid is measured by increased turbidity.

Answer 13

→ First statement is correct. Second statement is incorrect.

Question 14

Major sources of Gamma-glutamyl transferase includes the following:
1. Heart
2. Liver
3. Kidney
4. Pancreas

Answer 14

→ 2, 3, 4

Question 15

This is also known to its older terminology as Serum Glutamic oxaloacetic transaminase.

Answer 15

→ AST

Question 16

__________ : liberation of carbon dioxide from acidic acid; _________ : decrease in pH from acetic acid

Answer 16

→ Manometric ; Electrometric

Question 17

Kinetic Method has multiple measurement of absorbance change made at specific time interval.

Answer 17

→ True

Question 18

ALT determination involved LDH as coupling enzyme. Diagnostic significance is for the a specific evaluation of RBC disorders and skeletal involvement. → First statement is correct,

Answer 18

→ First statement is correct, second statement is incorrect

Question 19

Lactate Dehydrogenase has the highest activity in the heart, liver, skeletal muscle, kidney, and erythrocytes.

Answer 19

→ True

Question 20

Plate Assays for lipase determination uses tweens or tributyrin that change the color of phenol red after complexation with fluorescent dye Rhodamine B.

Answer 20

→ True

Question 21

Ptyalin : ___________ ; Amylopsin : __________

Answer 21

→ Salivary ; Pancreatic

Question 22

Amylase is falsely elevated upon administration opiates as painkiller. Pancreactic amylopsin secreted from the acinar cells of the pancreas.

Answer 22

→ Both statements are correct.

Question 23

Gamma-glutamyl transferase is the smallest enzyme.

Answer 23

→ False

Question 24

Amylase breaks down Starch and Glycogen.

Answer 24

→ True

Question 25

Lactate Dehydrogenase is widely distributed in tissue.

Answer 25

→ True

Question 26

This substance is poisonous when inhaled, swallowed or when absorbed through skin produced during the reaction under reagent preparation and stability.

Answer 26

→ p-nitrophenol

Question 27

Pyridoxal Phosphate (Vitamin B12) is the coenzyme for the AST reaction.

Answer 27

→ False

Question 28

Is the energy required to raise all molecules to the transition state in a chemical reaction so that products may be formed?

Answer 28

→ Activation energy

Question 29

Hemolysis should be avoided because hemoglobin inhibits amylase activity.

Answer 29

→ False

Question 30

CK is stable due to oxidation of sulfhydryl groups.

Answer 30

→ False

Question 31

Which is/are TRUE about Pseudocholinesterase determination:
1. Manometric method liberation of carbon dioxide from acetic acid
2. Cherry Krandal Method is the reference method
3. Decreased level is clinically significant

Answer 31

→ 1 & 3

Question 32

PChe : ___________ ; Ache : _______________

Answer 32

→ White matter of nervous system ; Brain

Question 33

Which is/are TRUE about Reitman and Frankel Method?
1. Color reaction is measured at 505nm due to diazonium salt coupled with keto acid.
2. 35 U/L – Within reference range

Answer 33

→ 1 only

Question 34

__________ : bind to active site ; ______________ : bind to ES complex

Answer 34

→ Competitive ; Uncompetitive

Question 35

Which among the following is a method of analysis for Lipase.
1. Amyloclastic
2. Chromogeninc
3. Saccharogenic
4. Titrimetric

Answer 35

→ None among the options

Question 36

__________ : removal of sialic acid ; ___________ : bind other isoenzymes

Answer 36

→ Neuramidase ; Wheat Germ Lectin

Question 37

Amylopsin is secreted from the Acinar Cells of the pancreas

Answer 37

→ True