(25) ADDITIONAL NOTES Flashcards
FOR REFERENCE RANGES
Refer to handout page 13
AST (SGOT) VS ALT (SGPT)
Refer to handout page 14
CONDITIONS OF ↑TRANSAMINASES
Refer to handout page 14
CK-MB – AST – LDH
Refer to handout page 14
↓absorbance: coenzyme is oxidized (Ex. NAD – w/out H) →
Product is colorless
↑absorbance: coenzyme is reduced (Ex. NADH – w H) →
Product has color
RULE: If absorbance is read between 400-700nm (visible light) →
we expect a colored product to be seen.
o <400: UV light → colorless rxn
o >700: IR light → colorless rxn
Aminotransferases (AST & ALT) require coenzyme.
Pyridoxal Phosphate (Vit. B6) is the coenzyme for aminotransferases
▪ Serves as prosthetic group (has tight attachment to apoenzyme)
1st enzyme that elevates in serum in pxs w/ AMI.
CK-MB
1st protein that elevates in serum in pxs w/ AMI
Myoglobin
both have the same forward & reverse assay rxn for the measurement of CK & LDH activity.
CK & LDH
Most heat-stable ALP isoenzyme
Placental isoenzyme
Most heat-stable ALP
Regan ALP
definitive test for Bone ALP.
Electrophoresis
definitive test for Placental ALP.
Heat denaturation
Pancreatic enzymes
ACP & G6PD
In continuous monitoring, the last enzyme is always an oxidoreductase beacuse
the conversion of coenzyme occurs at the last rxn.
Pxs during their deep sleep is where acute pancreatitis manifests
o Px during deep sleep moans due to extreme pain.
Under normal kidney conditions,
↑in plasma = ↓in urine.
- Anything excessive in plasma should be excreted in urine
- Anything deficient in plasma should be conserved, reabsorbed in kidney then back to plasma.
AMY elevates faster than LPS but LPS is more specific.
Why AMY? Bcos they are smaller.
- purer form of TAGs
- Substrate for LPS measurement
Triolein
Why are TAGs in water turbid?
bcos they are hydrophobic / insoluble to H2O.
Hgb can inhibit the enzymatic activity of LPS to the substrate.
That’s why hemolysis causes false decrease in LPS.
