(25) ADDITIONAL NOTES

Question 1

FOR REFERENCE RANGES

Answer 1

Refer to handout page 13

Question 2

AST (SGOT) VS ALT (SGPT)

Answer 2

Refer to handout page 14

Question 3

CONDITIONS OF ↑TRANSAMINASES

Answer 3

Refer to handout page 14

Question 4

CK-MB – AST – LDH

Answer 4

Refer to handout page 14

Question 5

↓absorbance: coenzyme is oxidized (Ex. NAD – w/out H) →

Answer 5

Product is colorless

Question 6

↑absorbance: coenzyme is reduced (Ex. NADH – w H) →

Answer 6

Product has color

Question 7

RULE: If absorbance is read between 400-700nm (visible light) →

Answer 7

we expect a colored product to be seen.

o <400: UV light → colorless rxn
o >700: IR light → colorless rxn

Question 8

Aminotransferases (AST & ALT) require coenzyme.

Answer 8

Pyridoxal Phosphate (Vit. B6) is the coenzyme for aminotransferases
▪ Serves as prosthetic group (has tight attachment to apoenzyme)

Question 9

1st enzyme that elevates in serum in pxs w/ AMI.

Answer 9

CK-MB

Question 10

1st protein that elevates in serum in pxs w/ AMI

Answer 10

Myoglobin

Question 11

both have the same forward & reverse assay rxn for the measurement of CK & LDH activity.

Answer 11

CK & LDH

Question 12

Most heat-stable ALP isoenzyme

Answer 12

Placental isoenzyme

Question 13

Most heat-stable ALP

Answer 13

Regan ALP

Question 14

definitive test for Bone ALP.

Answer 14

Electrophoresis

Question 15

definitive test for Placental ALP.

Answer 15

Heat denaturation

Question 16

Pancreatic enzymes

Answer 16

ACP & G6PD

Question 17

In continuous monitoring, the last enzyme is always an oxidoreductase beacuse

Answer 17

the conversion of coenzyme occurs at the last rxn.

Question 18

Pxs during their deep sleep is where acute pancreatitis manifests

Answer 18

o Px during deep sleep moans due to extreme pain.

Question 19

Under normal kidney conditions,

Answer 19

↑in plasma = ↓in urine.

Question 20

• Anything excessive in plasma should be excreted in urine

Answer 20

• Anything deficient in plasma should be conserved, reabsorbed in kidney then back to plasma.

Question 21

AMY elevates faster than LPS but LPS is more specific.

Answer 21

Why AMY? Bcos they are smaller.

Question 22

• purer form of TAGs
• Substrate for LPS measurement

Answer 22

Triolein

Question 23

Why are TAGs in water turbid?

Answer 23

bcos they are hydrophobic / insoluble to H2O.

Question 24

Hgb can inhibit the enzymatic activity of LPS to the substrate.

Answer 24

That’s why hemolysis causes false decrease in LPS.