(23) LPS - PANCREATIC ENZYMES

Question 1

LPS Systematic name:

Answer 1

Triacylglycerol Acylhydrolase

Question 2

• Earliest / most specific pancreatic marker
• Hydrolyzes the ester linkages of fats to produce alcohols & fatty acids

Answer 2

LPS (Lipopolysaccharide)

Question 3

main source of LPS

Answer 3

Pancreas

Question 3

o TAGs as substrate (hydrophobic – insoluble to H2O)
o 2-monoglyceride & Fatty acids as product (we measure the FA liberated from TAGs)

Answer 3

↑LPS activity = ↑fatty acids liberated from TAGs

Question 4

breaks down fats (TAG to monoglyceride)

Answer 4

Lipase

Question 5

Acute pancreatitis:

LPS rises: ____ after onset
Peaks: ____ after onset
Remains elevated for: _____

Answer 5

Acute pancreatitis:

LPS rises: 6hrs after onset
Peaks: 24hrs after onset
Remains elevated for: 7 days / 1 wk

Question 6

Reference method for LPS

Answer 6

Cherry Crandall

Question 7

Cherry Crandall Indicator

Answer 7

Phenolphthalein

Question 8

Tietz indicator

Answer 8

Thymolphthalein + Veronal

Question 9

Substrate of both Cherry Crandall & Tietz

Answer 9

50% Olive oil (triolein) – half is water bcos LPS is a hydrolase

Question 10

Titrating agent of both Cherry Crandall & Tietz

Answer 10

0.4N NaOH

Question 11

Cherry Crandall end color

Answer 11

Pink

Question 12

Tietz end color

Answer 12

Blue

Question 13

o Estimation of liberated fatty acids
o ↑TAGs = turbid soln
o Activity of LPS to triacyglycerol as substrate is inversely proportional to absorbance reading of soln.
▪ More active = ↑LPS activity to substrate = less turbid suspension = ↓absorbance

Answer 13

LPS Turbidimetric methods