(19) ACP - LIVER ENZYME

Question 1

ACP

Answer 1

Acid phosphatase

Question 2

• Same activity w/ ALP, only differ in pH
• Catalyze the hydrolysis of various phosphomonoesters at an acid pH
• Liberate inorganic phosphate from an organic phosphate ester with production
  of alcohol

Answer 2

ACP

Question 3

Sources of ACP

Answer 3

Prostate gland, RBCs, Platelets, Bones (Osteoclast)

Question 4

NOTE:

ACP Diagnostic significance:

Answer 4

o Old method to evaluate Prostate Metastatic Carcinoma.

• PSA - currently used to detect Prostate CA.

Question 5

Forensic investigation of rape

Answer 5

o Prostatic ACP can only be detected within 4 days (pero sabi ni sir 72hrs sksksks)

Question 6

Added to ACP to differentiate the prostatic form from the non-specific form like RBC acid

Answer 6

Phosphatase inhibitors

Question 7

inhibits specific prostatic ACP

Answer 7

L-tartrate ions

Question 8

inhibits red cell ACP

Answer 8

Formaldehyde & Cupric ions

Question 9

Assay for Enzyme activity for ACP

Answer 9

Bowers & McComb

Question 10

ACP Quantitative end point method

Answer 10

SUBSTRATE : Thymolphthalein monophosphate

Question 11

ACP Continuous monitoring method

Answer 11

SUBSTRATE : a-naphthyl phosphate

Question 12

GUTMAN & GUTMAN

Answer 12

SUBSTRATE : Phenyl PO4
END-PRODUCT : Inorg. phosphate

Question 13

SHINOWARA

Answer 13

SUBSTRATE : PNPP
END-PRODUCT : P-Nitrophenol

Question 14

BABSON, READ & PHILLIPS

Answer 14

SUBSTRATE : Alpha Naphtyl PO4
END-PRODUCT : Alpha Naphtol

Question 15

ROY & HILLMAN

Answer 15

SUBSTRATE :
Thymolphthalein MonoPO4
END-PRODUCT : Free Thymolphthalein