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BACTERIOLOGY - LEC > FINAL WAVE > Flashcards

FINAL WAVE Flashcards

1
Q

C. jejuni & C. coli are associated with?

A

gastritis and diarrhea

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2
Q

is campylobacter microaerophilic?
T or F?

A

T

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3
Q

campylobacters are capnophilic.
T or F

A

T

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4
Q

campylobacters are gram positive.
T or F

A

F (gram negative)

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5
Q

(campylobacter)
optimun temp for growth

A

42C ; 36-37C

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6
Q

campylobacter
motility

A

cork-screw darting

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7
Q

campylobacter flagellum
unipolar or bipolar

A

BOTH

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8
Q

campylobacter
culture media

A

Butzler
Skirrows
CAMPY-BAP
CCDA

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9
Q

blood used
CAMPY-BAP:
Skirrow’s:

A

5% sheep blood
5% horse blood

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10
Q

skirrow’s medium components

A

5% horse blood
vancomycin
polymyxin B
trimethoprim

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11
Q

Campy-BAP and campy thio antibiotic components

A

Vancomycin
Trimethoprim
Cephalotin
Polymyxin B
Amphotericin B

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12
Q

Campylobacter
specimens:

A

Feces
Blood
Rectal swabs for culture

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13
Q

Campylobacter
Medium used when spx is delayed for more than 2 hrs

A

Cary-Blair transport medium/ campy thio
Thioglycollate broth

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14
Q

Campylobacter
Spx temp storage until processed

A

4C

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15
Q

Campylobacter jejuni appearance in stained smear

A

Seagull-winged appearance

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16
Q

METHODS USED IN IDENTIFICATION OF CAMPYLOBACTER

A

PCR amplification
EIA

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17
Q

detection of Campylobacter spp. From clinical specimens

A

PRC amplification

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18
Q

used to detect antigens in stool samples for several days

A

EIA

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19
Q

CCDA

A

Charcoal Cefoperazone Deoxyxholate
Agar

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20
Q

Campy CVA

A

cephalothin,
vancomycin, and amphotericin B

21
Q

Helicobacter is associated with?

A

antral gastritis
duodenal (peptic) ulcer dx
gastric ulcers
gastric carcinome

22
Q

best spx for helicobacter diagnosis

A

antral biopsy

23
Q

Biopsy urease test
(+) :

A

Pink color

24
Q

Helicobacter
Antigen detection

A

Warthin-Starry or other silver stains and Giemsa
stains to examine biopsy specimens

urea breath test

analysis of stool samples before and after
antimicrobial therapy

25
Q

Helicobacter
Nononvasive moleculapr tests

A

PCR-based methods
Real-time PCR

26
Q

is the primary antibody found in your H. pylori
infection

27
Q

Helicobacter
techniques in antibody detection

A

ELISA
immunoblot

rapid tests using latex agglutination or
flow-through membrane-based enzyme
immunoassay

28
Q

agent of “whooping cough”

A

B. pertussis

29
Q

Bordet-Gengou bacillus

A

B. pertussis

30
Q

Bordetella
3 stages

A

Catarrhal
Paroxysmal
Convalescent

31
Q

prodromal stage
general flu-like symptoms

32
Q

repetitive coughing ep
whooping inhalation

A

paroxysmal

33
Q

recovery phase

A

convalescent

34
Q

rare complications in bordetalla

A

encephalitis

35
Q

bordetlla
ideal spx

A

nasopharyngeal aspirates or nasopharyngeal swab

36
Q

bodetalla
colonies

A

small, shiny colonies resembling tiny drop
of mercury

37
Q

has been found to work best for
recovery of B. pertussis from nasopharyngeal swabs

A

Regan-Lowe agar

38
Q

is superior to methicillin and penicillin for
inhibiting normal respiratory flora

A

cephalexin

39
Q

potato glycerol blood agar

A

Border-Gengou agar

40
Q

used not only
for the isolation of your Bordetella but also for the
transport

A

Modified Jones-Kendrick charcoal

41
Q

media used for the primary isolation of
Bordetella

A

Modified Stainer-Scholte
medium with cyclodextrin and
cephalothin

42
Q

best method of prevention for pertussis

A

vaccination

43
Q

cause of pertussis-like syndrome

A

B. parapertussis

44
Q

inhabits respiratory tract of
canines (kennel’s cough), infrequently responsible
for chronic respiratory tract infections in humans (rare
to cause infection in humans)

A

B. bronchiseptica

45
Q

undulant fever or malta fever

A

Brucellosis

46
Q

Brucella

humans acquire infections through

A

ingestion of
contaminated animal products, including meats
and milk; farmers can directly acquire infections
through direct animal contact

47
Q

Brucella
culture media

A

BAP , TSA, BACTEC 9240, BacT/Alert,
castaneda medium, Wisconsin medium, and TSB

48
Q

Brucella
Spx

A

blood, biopsy material, serum

BACTERIOLOGY - LEC (19 decks)

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