Final review 1 Flashcards

1
Q

Three characteristics of cells

A
  • cells are the basic unit of life
  • all organisms are made of cells
  • cells arise from other cells
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2
Q

What is the definition of evolution?

A

Random mutations that occur during the replication of the genome, combined with natural selection, are the basis for evolution of organisms

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3
Q

3 characteristics of evolution

A
  • Evolution always builds on existing structures, changes these structures or combines into new functions
    -Evolution cannot go back and start from scratch
    -Evolution is like a junkyard builder, designing new cars from pieces of old ones
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4
Q

What microscopes have a resolution (spacial) of 250nm?

A

Bright-field microscopy and Fluorescence Microscopy

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5
Q

What microscopes have a resolution (spacial) of 0.1nm?

A

Transmission EM
Scanning EM
Atomic Force Microscopy

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6
Q

What microscopy methods have a fast temporal resolution?

A

Bright-field microscopy and Fluorescence Microscopy
Atomic force microscopy

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7
Q

What microscopy requires cells to be fixed?

A

Transmission EM and scanning EM

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8
Q

What is the only microscopy that has a high specificity?

A

fluorescent microscopy

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9
Q

What type of microscopy views the cell surface?

A

Scanning EM and Atomic force

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10
Q

4 small building blocks of a cell

A

Sugar, fatty acids, amino acids, nucleotides

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11
Q

4 large building blocks of a cell

A

Polysaccharides, lipids, proteins, nucleic acids

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12
Q

What is the energy carrier of the cell?

A

ATP

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13
Q

What are two activated carrier of electrons?

A

NADH and NADPH

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14
Q

What is the difference between condensation and hydrolysis?

A

Condensation you loose water and hydrolysis you break a bond by adding water

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15
Q

Components of phospholipids

A

contain fatty acids, glycerol phosphate and polar head group

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16
Q

What polymerizes to form polypeptides (proteins)?

A

Amino acids

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17
Q

What can cause protein denaturation?

A

high temperatures or adding detergents

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18
Q

What happens when the interactions within a protein are weak?

A

The protein denatures

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19
Q

What are chaperones?

A

Help with protein folding

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20
Q

What does primary protein structure show?

A

Amino acid sequence

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21
Q

What does secondary protein structure show?

A

A helix and B sheet

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22
Q

What does tertiary protein structure show?

A

3D structure

23
Q

What does quaternary protein structure show?

A

Protein complex

24
Q

What stabilizes protein structures?

A

Disulfide bonds

25
What are larger protein structures called?
Protein complexes
26
What is required to be highly specific?
enzymes
27
What helps lower activation energy for a catalyzed reaction?
enzymes
28
What conversion is not favored?
X to Y
29
What is the result of coupling the conversion?
By coupling the favorable C to D, it can drive the X to Y reaction
30
What reaction is useful in helping drive reactions?
ATP to ADP hydrolysis
31
What is catabolism?
Energy production from food
32
What two cycles are key for the energy production from food
glycolysis and citric acid cycle
33
Where is the citric acid cycle occurring?
Mitochondria
34
What organelle is responsible for ATP production?
mitochondria
35
Where does ATP get its energy from?
NADH and FADH2 electrons converted into a proton gradient, the energy of the proton gradient is converted to ATP
36
What two cycles provide the precursors needed for cells to synthesize many important organic molecules?
Glycolysis and citric acid cycle
37
What two pathways are linked?
Catabolic and anabolic
38
Where does photosynthesis occur?
Chloroplast
39
Result of photosynthesis?
NADPH and ATP
40
What is the benefit of coupling the two photosystem?
to achieve a low enough redox potential to reduce NADP+
41
What is redox potential?
describes the ability of a molecule to accept of donate electrons
42
What are three properties of membranes?
- stable lipid bilayers form spontaneously in water - Stabilized by hydrophobic interactions - low permeability for charged molecules
43
When is lipid composition in membranes adjusted?
to temperature, to organelle, to function
44
What is osmolarity?
total concentration of all solute particles
45
What has higher osmolarity, cytoplasm or extracellular space?
cytoplasm
46
What causes turgor pressure?
cytoplasm having a higher osmolarity and pushing water into the cell
47
What allows water to pass through the cell more efficiently?
aquaporins
48
What is the concentration gradient?
Higher concentration of a molecule on one side of the membrane compared to the other side
49
What is membrane potential?
Unequal charge distribution across the membrane
50
What is the electrochemical gradient?
determines what direction the charged solutes will move
51
What are characteristics of a transporter?
contain a central binding site for the molecule. The binding site changes accessibility from one side of the membrane to the other, used for molecules/nutrients
52
What is a channel?
selective pores that allow ions to pass. electrochemical gradient determiens direction of flow
53
What is used for nutrient transport?
ion gradients