Fatty acid biosynthesis 1.1 Flashcards
Main use of lipids in cell structure?
Cellular membranes (plasma and organelle)
What can lower concentrations of lipids also be used for?
Signalling molecules
Example of a lipid signalling molecule?
Phosphatidyl inositol
What kind of reaction is the synthesis of fatty acid (OILRIG)?
Reduction
What is the major product of fatty acid synthesis?
Palmitic acid
Structure of palmitic acid?
CH3(CH2)14COOH
How many carbons do most naturally occuring fatty acids have (even/odd)?
even
Most even numbers of fatty acids are?
C14, 16, 18, 20
What kind of organism can produce fatty acids w/ an odd # of carbons?
Bacteria
How many carbon units are added to the growing fatty acid chain?
2
Where does fatty acid biosynthesis occur?
Cytoplasm
What coenzymes does fatty acid biosynthesis require?
NADP+ / NADPH
How is it known that fatty acid biosynthesis occurs in the cytosol?
Tissue was centrifuged and two proteins that are involved in FA biosynth came out in cytosol fraction
Stage one of fatty acid biosynthesis?
Carboxylation of acetyl-CoA
What is formed when acetyl-CoA is carboxylated?
Malonyl-CoA
What provides the CO2 for the carboxylation of acetyl-CoA?
HCO3 -
What molecule is needed for the first step of FA biosynthesis to occur?
biotin
What is the first part of the first step of FA biosynthesis?
Reaction between Biotin and HCO3 -
What is the useful product formed via the reaction between biotin and HCO3 -?
Biotin-CO2
What enzyme is used in the second part of the first step of fatty acid biosynthesis?
Transcarboxylase
What is the second step of fatty acid biosynthesis?
Chain elongation
How are long chain fatty acids formed?
Additions of acetyl-CoA and malonyl-CoA
Role of acetyl-CoA in chain extension?
Primes the reaction, and becomes the methyl terminus of the fatty acid chain
Role of malonyl-CoA in chain extension?
Each one provides a 2 carbon unit to extend the chain