Exam 2: Lymphocytes and Co-stimulatory Receptors

Question 1

Lymphocytes involved in innate immune response

Answer 1

Nk cells

Question 2

Lymphocytes involved in cellular immune response

Answer 2

T cells

Question 3

Lymphocytes involved in humoral immune response

Answer 3

B cells

Question 4

CD lymphocytes receptors for CD3 found in

Answer 4

T cells

Question 5

CD lymphocytes receptors for CD4

Answer 5

Helper t cells

Receptor for MHC II

Question 6

CD lymphocytes receptors for CD8

Answer 6

Cytotoxic T cells

Receptor for MHC I

Question 7

Integrins

Answer 7

Heterodimeric proteins that prolong and stabilize cell itneracitons and allows successful antigen recognition

Question 8

Selectins

Answer 8

regulate immigration of lymphocytes into tissues (P, L and E selectin)

Question 9

Lymphocyte Mitogens

Answer 9

sitmulate lymphocytes to divide

Question 10

These are molecules/receptors that can regulate the lymphocyte function:

a. Cytokines
b. Antibodies
c. Complement
d. Antigen
e. All teh above

Answer 10

E. all the above

Question 11

CD4 and CD8 are expressed on B cells and NK cells. T/F

Answer 11

False

Question 12

Helper T Cells Function

Answer 12

regulate immune response

Question 13

Cytotoxic T cells Function

Answer 13

Destroy cells expressing endogenous antigens

Question 14

Regulatory T Cells Function

Answer 14

Control everything

Question 15

B Cells Function

Answer 15

produce antibodies

Question 16

Immunoglobulin Superfamily Properties:

Answer 16

• found on all cell surfaces
• none have enzymatic activity
• many cellular responses are triggered by 2 interactions between 2 different members of the superfamily (TCR and MHC)

Question 17

TCR alpha/beta most abundant in

Answer 17

humans, mice and most non-ruminants

Question 18

TCR gamma/delta most abundant in

Answer 18

calves, lambs and piglets

Question 19

B1 integrins (CD29/CD49)

Answer 19

Fibronectin, laminin, Collagen

Question 20

B2 integrins (CD11/CD18)

Answer 20

ICAM-1

Question 21

Lymphocyte Mitogens

Answer 21

PHA- phytohemagglutinin, LPS- lipopolysaccharide, Con A- Concanvalin A, Pokeweed, BCG- Bacille Calmette-Guerin

Question 22

Complementary Determining Region

Answer 22

a binding groove that comes in contact with antigen on the variable domain of the TCR

Question 23

What are the signal transducing proteins on the T cell?

Answer 23

CD3 Complex: made up of 5 protein dimers (gamma, delta, epsilon, L, N)
*protein dimers

Question 24

80% of T cell CD3 complex protein sequence

Answer 24

alpha, beta- gamma, epsilon- delta epsilon- zeta, zeta

Question 25

CD4 on TH2 cells bind to

Answer 25

MHC II on APC

Question 26

CD8 on Tc Cells bind to

Answer 26

MHC I on virus infected or abnormal cells

Question 27

CD4/ CD8 function to

Answer 27

* promote T cell responses | * link T cell to APC, to ensure an active signal between the cells

Question 28

What part of the TCR-CD3 recognizes antigen and MHC?

Answer 28

TCR alpha

Question 29

What part of the TCR- CD3 recognizes antigen?

Answer 29

TCR beta, gamma, delta

Question 30

What part of the TCR- CD3 is the signal transducer?

Answer 30

CD3 gamma, delta, epsilon, zeta, nu

Question 31

what part of the TCR- CD3 recognizes MHC II?

Answer 31

CD4

Question 32

What part of the TCR-CD3 recognizes MHC I?

Answer 32

CD8

Question 33

CD80 is found on

Answer 33

dendritic cells, macrophages and activated B cells

Question 34

CD86 is found on

Answer 34

B cells

Question 35

The binding of antigen to TCR stimulates T cell expression of

Answer 35

CD40 ligand

Question 36

The CD40 ligand on the T cells stimulates expression of (blank) on the APC.

Answer 36

CD40

Question 37

The CD40 binding CD40 stimulates expression of (blank) on T cells and (blank) on APC.

Answer 37

CD28/152 on T cell | CD80/86 on APC

Question 38

Ligand CD 152 binding to CD 80/86 causes

Answer 38

T Cell suppression: production of in doleamine dioxygenase which destroy tryptophan. Absence of tryptophan --> T cells can't respond to antigen--> T cell response is terminated

Question 39

Ligand CD28 binding to CD86 causes

Answer 39

* eight-fold activation | * T cells IL-2: upregulate cell survival genes, increase energy metabolism, promotes T cell division

Question 40

The variable domain of the TCR is explicit for binding a specific antigen. T/F

Answer 40

False

Question 41

TCR, BCR, MHC1 and MHC2 are not members of the IG superfamily. T/F

Answer 41

False

Question 42

``` If a processed and presented antigen bind in a correct manner on a T cell. T cell is activated and initiates the immune response by: A.cytokine production B. Mitosis C. Cell differentiation D. All the above ```

Answer 42

D. All the above

Question 43

CD3 is found in all T Cells and it has a main role in the signal transduction into the T cell. T/F

Answer 43

True