exam 2 Flashcards
light microscope
visible light is passed through the specimen and then through the glass lenses. the lenses refract/bend the light in such a way that the image of the specimen is magnified as it is projected into the eye or camera
magnification
the ratio of an object’s image size to its real size
resolution
is a measure of the clarity of the image
it is the minimum distance two points can be separated and stil be distinguished as separate points
contrast
the difference in brightness between the light and dark areas of an image
organelles
the membrane enclosed structures within eukaryotic cells
electron microscope
focuses a beam of electrons through a specimen or onto its surface
cytoplasm
to the region between the nucleus and plasma membrane, liquid
plasma membrane
functions as a selective barrier that allows passage of enough oxygen, nutrients, and wastes to service the entire cell
microvilli
thin projections from the cell’s surface that increase surface area without an appreciable increase in volume
what increases faster
surface area or volume
volume
nucleus
contains most f the genes in the eukaryotic cell
nuclear envelope
encloses the nucleus separating its contents from the cytoplasm
double membrane
nuclear lamina
netlike array of protein filaments that maintains the shape of the nucleus by mechanically supporting the nuclear envlopoe
chromatin
the complex of dna and proteins making up chromosomes inside the nucleus
nucleolus
prominent structure which appears through the electron microscope as a mass of densley stained granules and fibers adjoining part of the chromatin
ribsonomal rna is synthesized from instructions in the dna
proteins imported from the cytoplasm are assembled with rRNA into large and small subunits of ribosomes
ribosomes
complexes made of ribosomal RNA and protein, are the cellular components that carry out protein synthesis
free ribosomes
suspended in cytoplasm
bound ribosomes
attached to the outside of the ER or nuclear envelope
endomembrane system
includes: nuclear envelope, ER, golgi, lysosomes, and various kinds of vesicles and vacuoles, and the plasma membrane
this system carries out a variety of tasks in the cell, including synthesis of proteins, transport of proteins into membranes and organelles or out of the cell, metabolism
vesicles
membrane sacs that transport things in the cell and out
endoplasmic reticulum
extensive network of membranes that it accounts for more than half of the total membrane in many eukaryotes
the er consists of a network of membranous tubules and sacs called cisternae. the er membrane separates the internal compartment of the er, called the er lumen (cavity) or cisternal space, from the cytosol
smooth er
no ribosomes
synthesis of lipids, metabolism of carbohydrates, detoxyfication of drugs and poisons, storage of calcium ions
rough er
has ribosomes
secretes proteins produced by bound ribosomes (most are glycoproteins)
also produces membrane
transport vesicles
vesicles in transit form one part of the cell to another
golgi apparatus
products of the ER are modified and stored and then sent to other destinations
consists of flattened membranous sacs-cisternae-looking like a stack of pita bread
cis and trans faces of golgi
cis face near the er where stuff comes in
trans face gives rise to vesicles that pinch off and travel to other sides
lysosome
membranous sac of hydrolytic enzymes that an animal cell uses to digest (hydrolyze) macromolecules
phagocytosis
engulfing smaller organisms or food particles
autophagy
lysosomes use their hydrolytic enzymes to recycle the cell’s own organic material
vacuoles
large vesicles derived from the er and golgi
integral part of cell’s endomembrane system
vacuolar membrane is selective in transporting solutes and the solution inside a vacuole differs in composition from the cytosol
food vacuoles
formed by phagocytosis
store food and break down food and things
contractile vacuoles
pump excess water out of the cell, thereby maintaining a suitable concentraton of ions and molecules inside the cell
central vacuole
plant cells
develops by the coalescence of smaller vacuoles
cell sap is the plant cell’s main repository of inoranic ions, including potassium and chloride
major role in the growth of plant cells
endosymbiont theory
that an early ancestor off eukaryotic cells engulfed an oxygen-using non photosythetic prokaryotic cell. eventually, the engulfed cell formed a relationship with the host cell in which it was enclosed, becoming an edosymbiont. over the course of evolution this merged into a eukaryotic cell with a mitochondria and later the same happened with a chloroplast, creating plant cell
mitochondria
sites of cellular respiration, the metabolic process that uses oxygen to generate atp by extracting energy from sugars, fats and other fuels
cristae
infoldings of the inner membrane
intermembrane space (mitochondria)
narrow region between hthe inner and outer membranes in mitochondria
mitochondrial matrix
enclosed by the inner membrane
contains many different enzymes as well as the mitochondrial dna and ribosomes
enzymes in the matrix catalyze some of the steps of cellular respiration
chloroplasts
found in plants and algae, sites of photosynthesis. these organelles convert solar energy to chemical energy by absorbing sunlight nd using it to drive the synthesis of organic compounds such as sugars from co2 and water
chlorophyll
green pigment in chloroplasts
thylakoids
flattened interconnected sacs
granum
each stack of thylakoids
stroma
fluid outside the thylakoids
containes chloroplast DNA and ribosomes as well as many enzymes
plastids
specialized family of closely related plant organelles
contains chloroplast
peroxisome
specialized metabolic compartment bounded by a single membrane
contain enzymes that remove H2 atoms from certain molecules and transfer them to oxygen (o2) producing hydrogen peroxide (H2O2)
detoxify alcohol and other harmful compounds by transferring h2 from the poisons to o2
also contains an enzyme tha converts h2o2 to water
cytoskeleton
network of fibers extending throughout the cytoplasm
major role in organizing the structures and activities of cell
motor proteins
interacts with the cytoskeleton, work together with plasma membrane molecules to allow whole cells to move along fibers outside the cell
cell motility
microtubules
hollow rods constructed from a globular protein called tubulin
a-tubulin and B-tubulin.
microtubules grow in length by adding tubulin dimers
shape and support cell and serve as tracks along which organelles equipped with motor proteins can move
centrosomes
region that is located near the nucleus and is considered a microtubule organizing center
these microtubules function as comporession resisting girders of the cytoskeleton
centrioles
inside the centrosome is a pair of these
each are composed of 9 sets of triplet microtubules arranged in a ring
before an animal cell divides, the centrioles replicate
flagella
limited to just one or a few per cell and are longer than cilia
has an undulating motion like the tail of a fish