Exam 1 Part 2

Question 1

confocal microscopy

Answer 1

a modification of florescence microscopy - allow for section of some light to reach eye - blocks out some of light - X-ray vision- focuses better

Question 2

Transmission electron microscopy

Answer 2

TEM - transmits electrons instead of light
Makes wavelengths smaller for better resolution
uses heavy metals

Question 3

Scanning electron microscopy

Answer 3

SEM
higher resolution than TEM
3D

Question 4

types of cells

Answer 4

prokaryotic and eukaryotic

Question 5

model systems

Answer 5

lots of offspring
fast life cycle
small, easy to maintain
easy to manipulate (genetics, behavior, environment)
history/well studied
live in culture
ethics
genetic variation (either a lot or not much)

Question 6

E coli

Answer 6

prok. single cell, easy to maintain, replicate every 20 minutes, circular DNA, easy to introduce foreign DNA
90% of DNA replication knowledge from E coli

Question 7

Saccharomyces cerevisiae

Answer 7

reproduce every 30 inutes
Eukaryotic
cell wall
fungus

Question 8

Arabidopsis thaliana

Answer 8

many offspring in 8-10 weeks

plant

Question 9

Drosophila melanogaster

Answer 9

genetic manipulation (know genes on chromosomes) many offspring - lots of phenotypes to observe

Question 10

Caenorhabditis elegans

Answer 10

know every cell - cell death

959 body cells

Question 11

Danio rerio

Answer 11

zebrafish
transparent embryos - easy to maintain, 200-300 egs/week
3 months gen. time
vertebrate

Question 12

Mus musculus

Answer 12

field mouse

mammal, good reproduction; small, most human genes in mouse - not much variation with inbred line