ETC and Oxidative Phosphorykation Flashcards
Energy rich reduced coenzymes
NADH
FADH2
Oxidative phosphorylation function
Conversion of conserved energy (NADH, FADH2) to high energy phosphate bonds of ATP
Where is the electron transport chain located in the cell
Inner mitochondrial membrane
Complexes of Inner mitochondrial membrane
Complexes I-IV -> electron transport chain
Complex v -> catalyze ATP
ETC goal
Transfer of electrons from electron donors to electron acceptors
The transfer of electrons in electron transport chain is coupled to
H+ transfer
End goal of electron transport chain
Oxidize NADH & FADH2 to donate electrons to oxygen which is reduced to H2O
Form of transfer of electrons
Hydride (H-)
Hydrogen atom (H)
Electron. (e-)
Major participants of electron transport chain ( set of 4 proteins)
Complex I-> NADH dehydrogenase
Complex II -> succinate dehydrogenase
Complex III-> cytochrome bc1 complex
Complex IV -> cytochrome oxidase
Inputs of energy into the electron transport chain
Input in complex I -> NADH from glycolysis, TCA cycle and fatty acids oxidation.
Input in complex II -H> FADH2 from TCA cycle and fatty acid oxidation
Situation where you have free energy drop which allow H+ pumping
ComplexI from NADH to CoQ
Complex III from CoQ to cyt c
Complex IV from cyt c to O2
What is a negative redox potential related to hydrogen
Lower affinity for electrons so donation of electrons to H+
What is a positive redox potential related to hydrogen
Higher affinity for electrons than hydrogen so would accept electrons from hydrogen
Inhibitors of electron transport chain
Painkillers Demerol/ rotenone
Barbiturate Amytal
Antimycin A
Cyanide
CO
Mobile electron carriersv
CoQ
Cyt C
