Epithelium Flashcards
What is the most commonly used stain in histology/histopathology?
Haemotoxylin & Eosin (H&E) NA = purple/blue Proteins = pink RBC = red
What stain is used to show basement membranes?
Jones
BM = black
What is Massons Trichrome stain used to identify?
Collagen = green
scar tissue
What does PAS stand for?
Periodic Acid Schiff
How do you see mucous on a cytology?
PAS staining
What is Elastic Verhoeff van Gieson stain used for?
Elastic tissues/elastin
How does IHC work?
Antigens are targeted with monoclonal antibodies - turns specific tissue/cell gold
List the basic tissue types in the body
epithelial
connective
muscular
nervous
Functions of epithelia
protection absorption interface between different compartments secretory units/ducts contractility
Simple epithelium
one cell layer thick
Pseudostratified epithelium
one cell layer thick with nuclei at different locations
looks stratified
Stratified epithelium
multiple cell layers thick
Squamous cell
“fried egg”
Cuboidal cell
looks like a block
Columnar cell
tall & thin
Goblet cells
columnar epithelia
produce mucin
have cilia
can’t see easily on H&E - they look clear
Layers of epidermis (top-bottom)
stratum corneum stratum granulosum stratum spinous stratum basale basement membrane
Transitional epithelium
stratified cuboidal
bladder
often in folds
stretchy
Basement membrane is
connective tissue secreted by epithelium
not penetrated by blood vessels
Best stain combination to see basement membrane
PAS & H&E
silver
Function of basement membrane
structure compartmentalization filter polarization tissue scaffolding
Where on an epithelial cell does a modification occur?
apical domain
Cilia function
move material across the surface of the cell
Microvilli function
increase surface area of cell for absorption
Types of cell adhesion
anchoring junctions
tight junctions
gap junctions
