Epithelium Flashcards

1
Q

What is the most commonly used stain in histology/histopathology?

A
Haemotoxylin & Eosin 
(H&E)
NA = purple/blue
Proteins = pink
RBC = red
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2
Q

What stain is used to show basement membranes?

A

Jones

BM = black

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3
Q

What is Massons Trichrome stain used to identify?

A

Collagen = green

scar tissue

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4
Q

What does PAS stand for?

A

Periodic Acid Schiff

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5
Q

How do you see mucous on a cytology?

A

PAS staining

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6
Q

What is Elastic Verhoeff van Gieson stain used for?

A

Elastic tissues/elastin

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7
Q

How does IHC work?

A

Antigens are targeted with monoclonal antibodies - turns specific tissue/cell gold

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8
Q

List the basic tissue types in the body

A

epithelial
connective
muscular
nervous

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9
Q

Functions of epithelia

A
protection
absorption
interface between different compartments 
secretory units/ducts
contractility
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10
Q

Simple epithelium

A

one cell layer thick

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11
Q

Pseudostratified epithelium

A

one cell layer thick with nuclei at different locations

looks stratified

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12
Q

Stratified epithelium

A

multiple cell layers thick

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13
Q

Squamous cell

A

“fried egg”

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14
Q

Cuboidal cell

A

looks like a block

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15
Q

Columnar cell

A

tall & thin

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16
Q

Goblet cells

A

columnar epithelia
produce mucin
have cilia
can’t see easily on H&E - they look clear

17
Q

Layers of epidermis (top-bottom)

A
stratum corneum
stratum granulosum
stratum spinous
stratum basale
basement membrane
18
Q

Transitional epithelium

A

stratified cuboidal
bladder
often in folds
stretchy

19
Q

Basement membrane is

A

connective tissue secreted by epithelium

not penetrated by blood vessels

20
Q

Best stain combination to see basement membrane

A

PAS & H&E

silver

21
Q

Function of basement membrane

A
structure
compartmentalization
filter
polarization
tissue scaffolding
22
Q

Where on an epithelial cell does a modification occur?

A

apical domain

23
Q

Cilia function

A

move material across the surface of the cell

24
Q

Microvilli function

A

increase surface area of cell for absorption

25
Q

Types of cell adhesion

A

anchoring junctions
tight junctions
gap junctions