Enzymes

Question 1

Enzyme and substrate come together in what phase

Answer 1

Lag face

Question 2

Non protein substances required for normal enzyme activity

Answer 2

Cofactor

Question 3

Protein portion of the enzyme

Answer 3

Apoenzyme

Question 4

Inorganic materials such as minerals, calcium, iron

Answer 4

Activator

Question 5

An organic substance that reversibly combines with a specific protein, the Apoenzyme, and with a substrate to form an active enzyme system

Answer 5

Coenzyme

Question 6

An active substance formed by a combination of coenzyme and Apoenzyme

Answer 6

Holoenzyme

Question 7

Enzyme with the similar enzymatic activity but differ in physical, biochemical, and immunologic characteristics

Answer 7

Isoenzyme

Question 8

What are the three phases of an enzymatic reaction

Answer 8

Lag Phase, Linear phase, substrate depletion phase

Question 9

What are the six main enzyme categories in the international Union of biochemistry classification system

Answer 9

Oxidoreductases, transferases, hydrolases, lyases, isomerases, ligases

Question 10

What are the three CK isoenzyme ‘s

Answer 10

CK-MM, CK-BB, CK-MB

Question 11

What is more specific in acute pancreatitis

Answer 11

Lipase

Question 12

What test is nonspecific for acute pancreatitis

Answer 12

Amylase

Question 13

Vmax is unchanged and km is increased

Answer 13

Competitive inhibitor

Question 14

Km is unaltered and Vmax is decreased

Answer 14

Non competitive inhibitor

Question 15

Vimax is decreased and km is decreased

Answer 15

Uncompetitive inhibitor

Question 16

Activation energy is

Answer 16

Decreased by enzymes

Question 17

Serum levels of total CK are elevated after an acute myocardial infarction and in which of the following conditions

Answer 17

Duchenne type muscular dystrophy

Question 18

What liver enzyme is the best to use to evaluate liver function

Answer 18

Alt

Question 19

Elevation of tissue enzymes in serum may be used to detect

Answer 19

Tissue necrosis or damage

Question 20

What tissue has the highest activity of both alt and AST relative in serum

Answer 20

Liver

Question 21

The LD isoenzyme pattern produced by air the worth recite leakage most resembles that seen in which disease state

Answer 21

Myocardial infarction

Question 22

The isoenzyme LD 4 and LD 5 are elevated in

Answer 22

Liver disease

Question 23

The chemical determination that is helpful in establishing the presence of seminal fluid

Answer 23

Acid phosphatase

Question 24

In competitive inhibition of an enzyme reaction the

Answer 24

Inhibitor binds to the enzyme at the same site as the substrate

Question 25

In the determination of lactate dehydrogenase at 340nm using pyruvate as a substrate one actually measures the

Answer 25

Decrease in nadh

Question 26

The protein portion of an enzyme complex is called the

Answer 26

Apoenzyme

Question 27

When a reaction is performed in zero kinetics the rate of reaction is

Answer 27

Independent of the substrate concentration

Question 28

Enzyme reaction rates are increased by increasing temperatures until they reach the point of denaturation at

Answer 28

40 to 60 degrees Celsius

Question 29

An example of using enzymes as reagents in The Clinical Laboratory is what method

Answer 29

The hexokinase glucose method

Question 30

Activity of enzymes in serum may be determined rather than concentration because

Answer 30

The amount of enzyme is too low to measure

Question 31

Which CK isoenzyme is elevated and muscle diseases

Answer 31

CK-MM

Question 32

Elevation of serum amylase and lipase is commonly seen in

Answer 32

Acute pancreatitis

Question 33

The saccharogenic method for amylase determinations measures

Answer 33

The amount of product produced

Question 34

What enzyme pattern is most diagnostic of duchenne type muscular dystrophy

Answer 34

Total CK level that is 50 to 100 times the ULN

Question 35

What pre-analytical error most commonly causes false increases in serum enzyme measurements

Answer 35

The serum was not separated from Red cells within 1 hour

Question 36

Which pre-analytical error most commonly causes false increases in serum enzyme measurements

Answer 36

The serum was not separated from red blood cells within 1 hour