Amino Acids And Proteins

Question 1

The three-dimensional spatial configuration of a single polypeptide chain as determined by disulfide linkages, hydrogen bonds, electrostatic attractions, and Van Der waals forces is referred to as the

Answer 1

Tertiary structure

Question 2

Person’s with alkaptonuria lack which enzyme

Answer 2

Homogentisate oxidase

Question 3

The plasma protein mainly responsible for maintaining colloidal osmotic pressure in Vivo is

Answer 3

Albumin

Question 4

The protein electrophoresis pattern of plasma as compared to serum reveals a

Answer 4

Fibrinogen Peak between the beta and gamma globulins

Question 5

One advantage of high-resolution agarose electrophoresis over lower current electrophoresis is

Answer 5

High-resolution produces detect monoclonal and iligoclonal bands at lower concentrations

Question 6

High serum total protein with high levels of both albumin and globulins is usually seen in

Answer 6

Dehydration

Question 7

The biuret reaction for total serum proteins is based upon reaction of

Answer 7

Cu2+ ions with peptide bonds

Question 8

Absence or a large decrease in the alpha-1 peak in serum electrophoresis suggest

Answer 8

Alpha 1 antitrypsin deficiency

Question 9

The Guthrie screening test for increased serum phenylalanine is based on

Answer 9

The microbiologic procedure in which phenyl alanine counteracts the effects of a metabolic antagonist on the growth of bacillus subtilis

Question 10

Poor protein caloric nutritional status is associated with

Answer 10

A decreased level of prealbumin

Question 11

The electrophoretic pattern of a plasma sample compared with a serum sample shows a

Answer 11

A sharp fibrinogen Peak

Question 12

The biuret reaction for the analysis of serum protein depends on the number of

Answer 12

Peptide bonds

Question 13

In electrophoresis of all proteins, when the sample is placed in an electric field connected to a buffer of pH 8.6, all of the proteins

Answer 13

Have a negative charge

Question 14

Maple syrup urine disease is characterized by an increase in which of the following urinary amino acids

Answer 14

Valine, leucine, isoleucine

Question 15

The cellulose acetate electrophoresis of pH 8.6 of serum proteins will show in order of migration beginning with the fastest migration as follows

Answer 15

Albumin, alpha 1 globulin, Alpha 2 globulin, beta globulin, gamma globulin

Question 16

What removes the amino group from an amino acid

Answer 16

Deamination

Question 17

Guthrie test inhibitor for phenylketonuria

Answer 17

Beta 2 thienylalanine

Question 18

Guthrie test inhibitor for maple syrup urine disease

Answer 18

4 azaleucine

Question 19

Guthrie test inhibitor for homocystinuria

Answer 19

L methionine sulfaximine

Question 20

Transfer of an amino group with the carboxyl group

Answer 20

Transamination

Question 21

Acute phase reactant proteins include all of the following

Answer 21

Alpha 1 antitrypsin, haptoglobin, fibrinogen

Question 22

Amino group and a carboxyl group bonded to the alpha carbon

Answer 22

Peptide bond

Question 23

Nutritional assessment with poor protein caloric status is associated with

Answer 23

A decreased level of prealbumin

Question 24

In what condition would a normal level of myoglobin be expected

Answer 24

Multiple myeloma

Question 25

An immunofixation protein electrophoresis is performed on serum from a patient with most common type of multiple myeloma. The resulting pattern reveals

Answer 25

Monoclonal bands of the IGG type

Question 26

A serum electrophoresis pattern of decreased albumin, increased alpha 1 and Alpha 2 globulin, and gamma globulins is normal

Answer 26

Acute inflammation

Question 27

Distinct oligoclonal bands in the gamma Zone on CSF protein electrophoresis are diagnostic of

Answer 27

Multiple sclerosis

Question 28

When protein is dissolved in a buffer solution, the pH of which is more alkaline than the PI, and an electric current is passed through the solution, the protein will act as

Answer 28

An anion and migrate to the anode

Question 29

In a patient with nephrotic syndrome the total protein levels in urine would be

Answer 29

Higher-than-normal

Question 30

Isoelectric focusing is the type of electrophoresis used to phenotype for alpha-1 antitrypsin deficiency. when the protein is Electrophorsed it migrates to

Answer 30

The site where the proteins net charge exceeds the pi

Question 31

A CSF albumin serum albumin ratio was reported at 9.8 in a patient. How is this best interpreted

Answer 31

The blood-brain barrier may be compromised leading to increased plasma albumin present in the CSF

Question 32

What CSF protein would be measured when investigating active demyelination in multiple sclerosis

Answer 32

Myelin basic protein