Amino Acids And Proteins Flashcards
Exam 1
The three-dimensional spatial configuration of a single polypeptide chain as determined by disulfide linkages, hydrogen bonds, electrostatic attractions, and Van Der waals forces is referred to as the
Tertiary structure
Person’s with alkaptonuria lack which enzyme
Homogentisate oxidase
The plasma protein mainly responsible for maintaining colloidal osmotic pressure in Vivo is
Albumin
The protein electrophoresis pattern of plasma as compared to serum reveals a
Fibrinogen Peak between the beta and gamma globulins
One advantage of high-resolution agarose electrophoresis over lower current electrophoresis is
High-resolution produces detect monoclonal and iligoclonal bands at lower concentrations
High serum total protein with high levels of both albumin and globulins is usually seen in
Dehydration
The biuret reaction for total serum proteins is based upon reaction of
Cu2+ ions with peptide bonds
Absence or a large decrease in the alpha-1 peak in serum electrophoresis suggest
Alpha 1 antitrypsin deficiency
The Guthrie screening test for increased serum phenylalanine is based on
The microbiologic procedure in which phenyl alanine counteracts the effects of a metabolic antagonist on the growth of bacillus subtilis
Poor protein caloric nutritional status is associated with
A decreased level of prealbumin
The electrophoretic pattern of a plasma sample compared with a serum sample shows a
A sharp fibrinogen Peak
The biuret reaction for the analysis of serum protein depends on the number of
Peptide bonds
In electrophoresis of all proteins, when the sample is placed in an electric field connected to a buffer of pH 8.6, all of the proteins
Have a negative charge
Maple syrup urine disease is characterized by an increase in which of the following urinary amino acids
Valine, leucine, isoleucine
The cellulose acetate electrophoresis of pH 8.6 of serum proteins will show in order of migration beginning with the fastest migration as follows
Albumin, alpha 1 globulin, Alpha 2 globulin, beta globulin, gamma globulin
What removes the amino group from an amino acid
Deamination
Guthrie test inhibitor for phenylketonuria
Beta 2 thienylalanine
Guthrie test inhibitor for maple syrup urine disease
4 azaleucine
Guthrie test inhibitor for homocystinuria
L methionine sulfaximine
Transfer of an amino group with the carboxyl group
Transamination
Acute phase reactant proteins include all of the following
Alpha 1 antitrypsin, haptoglobin, fibrinogen
Amino group and a carboxyl group bonded to the alpha carbon
Peptide bond
Nutritional assessment with poor protein caloric status is associated with
A decreased level of prealbumin
In what condition would a normal level of myoglobin be expected
Multiple myeloma
