Enzymes Flashcards
Enzymes
Enzymes are biological catalysts produced by all living cells. They speed up chemical reactions occurring in living organisms without being changed themselves.
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Enzymes are proteins that living cells produce from amino acids obtained from the diet in animals, or manufactured in plants. Without enzymes, chemical reactions would occur too slowly to maintain life.
Amylase
catalyses the breakdown of starch into sugars, mainly maltose. It is present in saliva, pancreatic juice and germinating seeds.
Properties of enzymes
- Enzymes are specific, i.e. each type of enzyme catalyses only one type of reaction.
- Enzymes work best at a particular temperature known as the optimum temperature. This is about 37 °C for human enzymes.
- High temperatures denature enzymes, i.e. the shape of the enzyme molecules changes so that they are inactivated. Enzymes start to be denatured at about 40 °C to 45 °C.
- Enzymes work best at a particular pH known as the optimum pH. This is about pH7 for most enzymes
Extremes of acidity or alkalinity denature most enzymes
. * The action of enzymes is helped by certain vitamins and minerals, e.g. vitamin B1 helps the action of respiratory enzymes.
- The action of enzymes is inhibited by certain poisons, e.g. arsenic and cyanide.
enzymes
change substrates into products
e.g. starch is the substrate the product is maltose and the enzyme is amylase
all enzymes have a dent in them called its active site. it is the exact size and shape to fit the molecule it breaks down, enzymes have an active site that perfectly fits its substrate, it is unique to that partiular kind of molecule, high temepratures change the shape of the enzyme and the active site no longer fits its substrate denaturing them
sugars, fats and lipids, proteins
soluble
insoluble
some are soluble some are insoluble
carbs- C H O
fats- C H O
proteins- C H O N
enzymes at optimum temp or high temperatures rather than low
collsion theory, able to bump in more frequently to its substrate in high temps due to increased kinectic energy, low temps does slower movements, not as rapid and random/erratic
when asked to design a lab
pH- buffer solution
say how to observe results
to note colour changes
tables, graphs
calculations, etc
enxymes in humans plants and bacteriua in hot spings
in humans optimum temperature of 37 body temp
in plants optimum temp of 28 to 30
in bacteria living in hot springs optimum temp as high as 75
how to read graphs
as _____ increases the rate of the enzyme activity ____increases
as _____ increases the rate of the enzyme activity ____(perhaps rapidly)decreases
enzymes in humans start to denature at 40 to 45