Enzyme: how fast product is made - Required Practicals

Question 1

What does catalase break down

Answer 1

H2O2 → H2O + O2

Question 2

As oxygen is produced in the reaction where does the water go

Answer 2

The oxygen displaces the water in the measuring cylinder

Question 3

Outline method for measuring rate of enzyme action for catalase

Answer 3

1. Set up boiling tubes with the same vol and conc of H2O2 and another set of boiling tubes with catalase in.
2. Add equal volumes of buffer to each test tube
3. Set up apparatus: trough of water, upturned measuring cylinder, delivery tube held by clamp.
4. Put each boiling tube and another set of boiling tubes with catalase set to 10 - 40
5. Wait 5 mins
6. Pipette same volume of catalase to each of the boiling tubes
7. Attach bung and delivery tube to test tube
8. Record more oxygen is produced in first minute using stop watch
9. Repeat and calculate average amount produced
10. Calculate rate

Question 4

What would a negative control be in this experiment

Answer 4

A boiling tube containing without adding catalase for each temp

Question 5

Why is a buffer used

Answer 5

Keep ph constant

Question 6

Why is the catalase also put into the water bath

Answer 6

To see if the enzyme is denatured by temp