Entero: E. coli (Ex2) Flashcards

1
Q

Where does Enterobacteriacae normally reside?

What are the benefits of it being there?

A
  • Resides in the GI tract
  • Microbial antagonism
  • Breakdown and absorption of food
  • Waste processing
  • Vitamin K production
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2
Q

General features of Enterobacteriacae

gram, oxygen requirements, shape, oxidase

A
  • Gram negative
  • facultative anaerobe
  • rods or coccobacilli
  • oxidase negative
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3
Q

What are Coliform Bacteria?

Name the three included

A
  • Lactose positive bacteria
  • E. coli
  • Klehsiella
  • Enterobacter
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4
Q

General features of Escherichia coli

lactose, oxidase, motility, shape

A
  • lactose positive
  • oxidase negative
  • motile
  • rods
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5
Q

Virulence Factors of E. coli

A
  • capsule
  • fimbrial and non-fimbrial adhesions
  • endotoxin (LPS)
  • enterotoxins
  • cytotoxins
  • siderophores
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6
Q
What do these stand for?
ETEC
EHEC
VTEC/STEC
EPEC
RPEC
APEC
NTEC
A
ETEC - enterotoxigenic
EHEC - enterohemorrhagic
VTEC/STEC - verotoxigenic/shiga-like toxic
EPEC - enteropathic
RPEC - rabbit pathogenic
APEC - avian pathogenic
NTEC - nectrotoxigenic
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7
Q

General Features of ETEC in pigs

symptoms, age affected, adhesion factors, enterotoxins

A
  • watery diarrhea
  • neonatal, weanlings
  • adhesion factors: F4, F5, F6, F18, F41
  • enterotoxins: LT, Sta, Stb, EAST1
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8
Q

Adhesion factors of ETEC in pigs

A

F4 - on plasmids, specific receptors, mannose resistant, genes expressed at body temp, solely in pigs, neonates to weanlings
F5 - on plasmids, in pigs and bovines, mannose resistant, expression temp dependent, in young animals
F6 - extrachromosomal, neonatal
F41 - chromosomal, mannose resistant, pigs and bovines, freq with F5, rare, neonatal

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9
Q

Describe LT enterotoxin in ETEC in pigs

A
  • heat labile
  • high molecular weight
  • strong antigen
  • Na absorption, Cl and HCO3 secretion
  • activation of adenlyate cyclase
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10
Q

Describe the heat stable enterotoxins in ETEC in pigs

A
  • low molecular weight
  • little antigen
  • Sta: activation of guanylate cyclase, Na absorption, Cl and HCO3 secretion
  • Stb: prostaglandin E2, secretion of water and electrolytes, stimulation of 5-H secretion
  • EAST1: closely related to Sta
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11
Q

Characteristics present at each age (neonatal, neonatal till 4 wks, weanling) in ETEC in pigs
(adhesion, enterotoxins, hemo/non)

A

Neonatal: F5, F6, F41; Sta, Stb; non-hemolytic
Neonatal - 4 wks: F4; LT, Sta, STb, EAST1; hemolytic
Weanling: F4, F18; LT, Sta, Stb, EAST1; hemolytic

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12
Q

What are the causes of ETEC in pigs?

A
  • increase infection pressure
  • lowering of maternal immunity (MMA, first delivery, low birth weight, too many piglets)
  • appearance of new type
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13
Q

What are the symptoms of ETEC in pigs?

A
  • remain drinking
  • smelly feces
  • dehydration
  • diarrhea
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14
Q

How do you diagnose ETEC in pigs?

A
  • agglutination tests

- PCR

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15
Q

Treatment of ETEC in pigs

A
  • hydration

- antimicrobials

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16
Q

Prevention of ETEC in pigs

A
  • keep infection pressure low and maternal immunity high
  • hygiene
  • vaccination of sows
  • selection of receptor free piglets
17
Q

Virulence factors of VTEC in pigs

A
  • adhesion in small bowel: F18
  • exotoxin: VT2e or Stx2e: media necrosis
  • endotoxin: acute mortality
18
Q

What are the symptoms of VTEC in pigs?

A
  • acute mortality in some
  • diarrhea in some
  • anorexia
  • edema (hoarse voice, dyspnea, swollen eyelids, CNS)
  • CNS signs
19
Q

How do you treat VTEC in pig?

A
  • fasting to eliminate toxin
  • increases intestinal peristalsis
  • antimicrobial therapy
20
Q

How do you prevent VTEC in pigs?

A
  • reduce stress
  • feed restriction, increase fiber content, starter feed before weaning
  • adaptation of intestinal flora (probiotics, organic acids, zinc oxide, antimicrobials, vaccination)
  • selection of F18 receptor negative animals
21
Q

What are the symptoms of UTI E. coli in pigs?

A
  • anorexia
  • hematuria
  • vaginal exudate
  • general malaise
22
Q

What is MMA of PPDS?

What are the possible etiologies?

A
  • Metritis-Mastitis-Agalactiae
  • Post Partum Dysgalactiae Syndrome
  • problem in milk production
  • E: hereditary, hormonal, feed, infectious agents
23
Q

What are the symptoms of ETEC in bovines?

What age is affected?

A
  • watery diarrhea leading to dehydration

- less than 3 days old, neonates

24
Q

Virulence factors of ETEC in bovines

A
  • fimbriae (F5, F41, F17, CS31A)

- toxins (Sta and Stb)

25
EPEC in pigs | symptoms, mechanisms, diagnosis
- diarrhea at weaning - attachment to enterocytes, effacement of microvilli - type 3 secretion system: injection of effector proteins into host cell - diagnose by isolation and PCR
26
How to treat bovines with ETEC. | On milking farms?
- separate ill animals - hydration - antibiotics - NSAID (for shock) - MF: separate calves from mother, colostrum from another, antibiotics, vaccinate moms
27
What age does VTEC (Edema disease) affect piglets?
post weaning
28
What media necrosis by the toxin produced by VTEC in each chronicity of infect? (hyperacute, acute, chronic)
Hyperacute: enterorrhagia Acute: permeability of blood vessels leading to edema Chronic: intravascular coagulation
29
Describe the pathogenesis of ETEC in bovines
- oral uptake - multiplication - enterotoxin production - secondary septicemia
30
Diagnosis of ETEC in bovines
- ELISA | - culture+virulence factors (agglutination or PCR)
31
EPEC and EHEC in bovines | age, virulence factors, symptom, location, therapy
- 1-8 weeks - muccoid diarrhea +/- blood - large intestine - antibiotics - EPEC: eae - EHEC: eae, VT, zoonotic
32
Virulence factors of NTEC in bovines
- serum resistance - iron uptake - endotoxin - capsule - adhesion factors - CNF toxins
33
How does the cow become infected with NTEC?
- per os via lymphoid tissues of head and throat or intestine - via umbilical cord - too little colostrum
34
Symptoms of NTEC in bovines at each level of chronicity
Hyperacute: acute mortality, shock Acute: general symptoms, fast mortality Chronic: slow with organ localization
35
How do you treat NTEC in bovines
- NSAID (shock) - antibiotics - gamma globulins
36
Describe the pathogenesis of Neonatal colibacillosis (in avians)
- contamination of egg shell (shrinking of content and entrance of bacteria), neonatal contamination, manipulation of chicks - yolk rest infection - sepsis (then death), polyserositis, airsac, pericad (growth retardation)