Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Bacteriolgy > Entero: E. coli (Ex2) > Flashcards

Entero: E. coli (Ex2) Flashcards

1
Q

Where does Enterobacteriacae normally reside?

What are the benefits of it being there?

A
  • Resides in the GI tract
  • Microbial antagonism
  • Breakdown and absorption of food
  • Waste processing
  • Vitamin K production
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

General features of Enterobacteriacae

gram, oxygen requirements, shape, oxidase

A
  • Gram negative
  • facultative anaerobe
  • rods or coccobacilli
  • oxidase negative
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What are Coliform Bacteria?

Name the three included

A
  • Lactose positive bacteria
  • E. coli
  • Klehsiella
  • Enterobacter
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

General features of Escherichia coli

lactose, oxidase, motility, shape

A
  • lactose positive
  • oxidase negative
  • motile
  • rods
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Virulence Factors of E. coli

A
  • capsule
  • fimbrial and non-fimbrial adhesions
  • endotoxin (LPS)
  • enterotoxins
  • cytotoxins
  • siderophores
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q 
What do these stand for?
ETEC
EHEC
VTEC/STEC
EPEC
RPEC
APEC
NTEC
A 
ETEC - enterotoxigenic
EHEC - enterohemorrhagic
VTEC/STEC - verotoxigenic/shiga-like toxic
EPEC - enteropathic
RPEC - rabbit pathogenic
APEC - avian pathogenic
NTEC - nectrotoxigenic
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

General Features of ETEC in pigs

symptoms, age affected, adhesion factors, enterotoxins

A
  • watery diarrhea
  • neonatal, weanlings
  • adhesion factors: F4, F5, F6, F18, F41
  • enterotoxins: LT, Sta, Stb, EAST1
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Adhesion factors of ETEC in pigs

A

F4 - on plasmids, specific receptors, mannose resistant, genes expressed at body temp, solely in pigs, neonates to weanlings
F5 - on plasmids, in pigs and bovines, mannose resistant, expression temp dependent, in young animals
F6 - extrachromosomal, neonatal
F41 - chromosomal, mannose resistant, pigs and bovines, freq with F5, rare, neonatal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Describe LT enterotoxin in ETEC in pigs

A
  • heat labile
  • high molecular weight
  • strong antigen
  • Na absorption, Cl and HCO3 secretion
  • activation of adenlyate cyclase
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Describe the heat stable enterotoxins in ETEC in pigs

A
  • low molecular weight
  • little antigen
  • Sta: activation of guanylate cyclase, Na absorption, Cl and HCO3 secretion
  • Stb: prostaglandin E2, secretion of water and electrolytes, stimulation of 5-H secretion
  • EAST1: closely related to Sta
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Characteristics present at each age (neonatal, neonatal till 4 wks, weanling) in ETEC in pigs
(adhesion, enterotoxins, hemo/non)

A

Neonatal: F5, F6, F41; Sta, Stb; non-hemolytic
Neonatal - 4 wks: F4; LT, Sta, STb, EAST1; hemolytic
Weanling: F4, F18; LT, Sta, Stb, EAST1; hemolytic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What are the causes of ETEC in pigs?

A
  • increase infection pressure
  • lowering of maternal immunity (MMA, first delivery, low birth weight, too many piglets)
  • appearance of new type
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What are the symptoms of ETEC in pigs?

A
  • remain drinking
  • smelly feces
  • dehydration
  • diarrhea
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

How do you diagnose ETEC in pigs?

A
  • agglutination tests

- PCR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Treatment of ETEC in pigs

A
  • hydration

- antimicrobials

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Prevention of ETEC in pigs

A
  • keep infection pressure low and maternal immunity high
  • hygiene
  • vaccination of sows
  • selection of receptor free piglets
17
Q

Virulence factors of VTEC in pigs

A
  • adhesion in small bowel: F18
  • exotoxin: VT2e or Stx2e: media necrosis
  • endotoxin: acute mortality
18
Q

What are the symptoms of VTEC in pigs?

A
  • acute mortality in some
  • diarrhea in some
  • anorexia
  • edema (hoarse voice, dyspnea, swollen eyelids, CNS)
  • CNS signs
19
Q

How do you treat VTEC in pig?

A
  • fasting to eliminate toxin
  • increases intestinal peristalsis
  • antimicrobial therapy
20
Q

How do you prevent VTEC in pigs?

A
  • reduce stress
  • feed restriction, increase fiber content, starter feed before weaning
  • adaptation of intestinal flora (probiotics, organic acids, zinc oxide, antimicrobials, vaccination)
  • selection of F18 receptor negative animals
21
Q

What are the symptoms of UTI E. coli in pigs?

A
  • anorexia
  • hematuria
  • vaginal exudate
  • general malaise
22
Q

What is MMA of PPDS?

What are the possible etiologies?

A
  • Metritis-Mastitis-Agalactiae
  • Post Partum Dysgalactiae Syndrome
  • problem in milk production
  • E: hereditary, hormonal, feed, infectious agents
23
Q

What are the symptoms of ETEC in bovines?

What age is affected?

A
  • watery diarrhea leading to dehydration

- less than 3 days old, neonates

24
Q

Virulence factors of ETEC in bovines

A
  • fimbriae (F5, F41, F17, CS31A)

- toxins (Sta and Stb)

25
Q

EPEC in pigs

symptoms, mechanisms, diagnosis

A
  • diarrhea at weaning
  • attachment to enterocytes, effacement of microvilli
  • type 3 secretion system: injection of effector proteins into host cell
  • diagnose by isolation and PCR
26
Q

How to treat bovines with ETEC.

On milking farms?

A
  • separate ill animals
  • hydration
  • antibiotics
  • NSAID (for shock)
  • MF: separate calves from mother, colostrum from another, antibiotics, vaccinate moms
27
Q

What age does VTEC (Edema disease) affect piglets?

A

post weaning

28
Q

What media necrosis by the toxin produced by VTEC in each chronicity of infect?
(hyperacute, acute, chronic)

A

Hyperacute: enterorrhagia
Acute: permeability of blood vessels leading to edema
Chronic: intravascular coagulation

29
Q

Describe the pathogenesis of ETEC in bovines

A
  • oral uptake
  • multiplication
  • enterotoxin production
  • secondary septicemia
30
Q

Diagnosis of ETEC in bovines

A
  • ELISA

- culture+virulence factors (agglutination or PCR)

31
Q

EPEC and EHEC in bovines

age, virulence factors, symptom, location, therapy

A
  • 1-8 weeks
  • muccoid diarrhea +/- blood
  • large intestine
  • antibiotics
  • EPEC: eae
  • EHEC: eae, VT, zoonotic
32
Q

Virulence factors of NTEC in bovines

A
  • serum resistance
  • iron uptake
  • endotoxin
  • capsule
  • adhesion factors
  • CNF toxins
33
Q

How does the cow become infected with NTEC?

A
  • per os via lymphoid tissues of head and throat or intestine
  • via umbilical cord
  • too little colostrum
34
Q

Symptoms of NTEC in bovines at each level of chronicity

A

Hyperacute: acute mortality, shock
Acute: general symptoms, fast mortality
Chronic: slow with organ localization

35
Q

How do you treat NTEC in bovines

A
  • NSAID (shock)
  • antibiotics
  • gamma globulins
36
Q

Describe the pathogenesis of Neonatal colibacillosis (in avians)

A
  • contamination of egg shell (shrinking of content and entrance of bacteria), neonatal contamination, manipulation of chicks
  • yolk rest infection
  • sepsis (then death), polyserositis, airsac, pericad (growth retardation)

Bacteriolgy (17 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions