E. coli

Question 1

Enterobacteriaceae

Answer 1

family of bacteria with over 30 genera - some are commensal, some are virulent

Question 2

Enteric Enterobacteriaceae Pathogens

Answer 2

E. coli
Salmonella
Shigella

Question 3

Extraintestinal Enterobacteriaceae Pathogens

Answer 3

E. coli (yes it made both lists)
Klebsiella

Question 4

coliforms

Answer 4

gram-negative, non-spore
forming Bacilli, which possess ß-
galactosidase to produce acids and gases

Question 5

Serogroups of E. coli (4)

Answer 5

1. O Antigen
2. K Antigen
3. H antigen
4. F antigen

Question 6

O Antigen

Answer 6

encodes for the LPS type; very diverse

Question 7

K Antigen

Answer 7

encodes the capsule type

Question 8

H antigen

Answer 8

flagella type

Question 9

F antigen

Answer 9

fimbrial adhesins; sometimes referred to with older terminology (like K)

Question 10

Serotype of E. coli

Answer 10

combination of the serogroups, such as O157:H13, NM used for non-motile (no H antigen)

Question 11

Are serotypes of E. coli host-specific?

Answer 11

yes

Question 12

Why are we concerned about zoonotic disease with E. coli if the serotypes are host specific?

Answer 12

because the toxin can be zoonotic!

Question 13

Antibiotic Resistance in family Enterobacteriaceae

Answer 13

plasmids can be freely exchanged amongst the family so this is a huge concern with treatments

Question 14

Route of Transmission of E. coli

Answer 14

fecal-oral

Question 15

virulence factor

Answer 15

anything that promotes the growth of bacteria to enhance its survival in the host

Question 16

How is E. coli like Nelly?

Answer 16

great at responding to selective pressures to obtain diverse elements that improve their fitness and ability to survive in the host

Question 17

pili

Answer 17

protein structures important in bacterial conjugation for transfer of DNA

Question 18

Major E. coli Virulence Types

Answer 18

1. Enterotoxigenic (ETEC)
2. Attaching effacing (AEEC)
3. Enteroaggregative (EAEC)
4. Enteroinvasive (EIEC)

Question 19

2 Categories of Attaching Effacing Virulence of E. coli

Answer 19

1. Enterohemorrhagic (EHEC)
2. Enteropathogenic (EPEC)

Question 20

Which virulence types are diarrheal?

Answer 20

ETEC and EHEC and EPEC

Question 21

What virulent type causes the least amount of diarrhea?

Answer 21

EHEC

Question 22

Type of diarrhea caused by ETEC and EAEC?

Answer 22

watery/hypersecretory

Question 23

Type of Diarrhea caused by EPEC and EHEC

Answer 23

Mucoid/malabsoprtive

Question 24

Enteroaggregative

Answer 24

makes structure on the surface of enterocytes like biofilms

Question 25

Virulence types that cause Hemorrhagic Enteritis?

Answer 25

EHEC and EIEC

Question 26

colibacillosis

Answer 26

infection in swine, cattle, and poultry caused by ETEC, causes scours

Question 27

Why does ETC cause watery diarrhea?

Answer 27

has a heat-labile and heat-stable toxin which causes electrolyte loss and/or shift resulting in fluid changes, therefore watery diarrhea

Question 28

Importance of fimbrial adhesion?

Answer 28

prevents washout from the intestinal surface, allowing colonization

Question 29

EPEC affects primarily what species?

Answer 29

rabbits

Question 30

EPEC MOA

Answer 30

E. coli injects Tir into the host cell via T3SS, binds to the cell membrane of the host and then Tir interacts with actin, inserts itself in the host cell membrane, and binds to the intimin protein that is on the cell surface of the E. coli

Question 31

Tir

Answer 31

transfer of intimin receptor

Question 32

intimin

Answer 32

facilitates adherence

Question 33

Any toxins in EPEC?

Answer 33

no

Question 34

Shiga-like toxin

Answer 34

found in EHEC; inhibits protein synthesis and forces cell to rearrange its cell surface and form cups and pedestals

Question 35

Does EHEC cause diarrhea?

Answer 35

very little if at all (mucoid)

Question 36

Primary consequence of EHEC

Answer 36

bladder infections, gut and neural edema, swollen eyelids, CNS signs

Question 37

EAST

Answer 37

uniquely stable toxin found in swine and calves with EAEC