DNA Replication Flashcards
First step in DNA replication
Unwind DNA
What enzyme unwinds DNA
helicase
Second step of DNA replication
Build the daughter strand
-this adds new complementary bases
Where does energy for bonding usually come from?
ATP then AMP
Bonding by enzyme, which enzyme?
DNA Polymerase III
Leading and lagging strands, mummified by what? And how does the stand grow?
- limits of DNA polymerase III
- can Only build onto 3’end of an existing DNA strand
Lagging strand
- Okazai fragments
- joined by ligase
Leading strand
Continuous synthesis
The central dogma
- Flow of a genetic information in a cell
- Goes DNA, replication, transcription, RNA, translation, protein, trait
Transcription
he process by which genetic information represented by a sequence of DNA nucleotides is copied into newly synthesized molecules of RNA, with the DNA serving as a template.
RNA polymerase I
- Only transcribes RNA genes
- makes ribosomes
RNA Polymerase II
Transcribes genes into mRNA
RNA Polymerase III
- Only transcribes tRNA genes
- each has a specific pro meter sequins it recognizes
Promoter region
Binding site before beginning of gene
Tata box binding site
Bonding binding site for RNA primers and transcription factors