DNA Replication Flashcards
What is DNA replication
Describe the beginning of DNA replication in bacteria
What are the functions of topoisomerase I and II
What is semi-discontinuous replication
What is the structure and function of DNA polymerase III
Major polymerase
-Synthesizes leading strand and okazaki fragments
-Requires RNA primers to begin synthesis
-> One at start of leading strand
-> multiple in lagging strand (due to lagging template running 3’-5’ away from replication fork)
-Primase: RNA polymerase that synthesizes RNA primers
What is the structure and function of DNA polymerase I
-Removes RNA primers
-Fills gaps between Okazaki fragments
-Strands sealed by DNA ligase
What are the enzymes involved at the replication fork
Describe initiation of replication in eukaryotic cells
Describe the eukaryotic replication fork
What is the role of telomerase in the lagging strand
Differentiate prokaryotic vs eukaryotic DNA replication
Explain DNA repair
