Difficult subjects

Question 1

What is a gene?

Answer 1

A specific length of DNA which codes for a specific protein

Question 2

Why must temperature be controlled in a fermenter?

Answer 2

1. Enzymes denature

2. Best conditions for growth

Question 3

What must be controlled Ina fermenter and why?

Answer 3

1. Oxygen

2. For respiration

Question 4

How do nitrate ions help growth?

Answer 4

1. Active transport
2. Low to high concentration
3. Requires energy

Question 5

When doing energy calculations, why would using a larger water volume be more accurate?

Answer 5

1. Smaller surface area:volume

2. Lose heat less easily

Question 6

What is the test for glucose?

Answer 6

1. Add Benedicts and heat
2. Changes colour
3. Changes depending on amount of glucose

Question 7

how can you produce hormones?

Answer 7

1. use a restriction enzyme to cut genes out of human DNA
2. remove a plasmid from bacteria and open it using restriction enzymes
3. use ligase to stick the genes to the plasmid
4. return the plasmid